Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake

Nicolin Tee,1 Yingdong Zhu,2 Gysell M Mortimer,1 Darren J Martin,2 Rodney F Minchin11School of Biomedical Science, University of Queensland, Brisbane, QLD, Australia; 2Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD, AustraliaAbst...

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Autores principales:	Tee N, Zhu Y, Mortimer GM, Martin DJ, Minchin RF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1f414d5ad9aa4e0883e5e405e415775d
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spelling	oai:doaj.org-article:1f414d5ad9aa4e0883e5e405e415775d2021-12-02T05:36:19ZFluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake1178-2013https://doaj.org/article/1f414d5ad9aa4e0883e5e405e415775d2015-03-01T00:00:00Zhttp://www.dovepress.com/fluoromica-nanoparticle-cytotoxicity-in-macrophages-decreases-with-siz-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Nicolin Tee,1 Yingdong Zhu,2 Gysell M Mortimer,1 Darren J Martin,2 Rodney F Minchin11School of Biomedical Science, University of Queensland, Brisbane, QLD, Australia; 2Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD, AustraliaAbstract: Polyurethanes are widely used in biomedical devices such as heart valves, pacemaker leads, catheters, vascular devices, and surgical dressings because of their excellent mechanical properties and good biocompatibility. Layered silicate nanoparticles can significantly increase tensile strength and breaking strain of polyurethanes potentially increasing the life span of biomedical devices that suffer from wear in vivo. However, very little is known about how these nanoparticles interact with proteins and cells and how they might exert unwanted effects. A series of fluoromica nanoparticles ranging in platelet size from 90 to over 600 nm in diameter were generated from the same base material ME100 by high energy milling and differential centrifugation. The cytotoxicity of the resulting particles was dependent on platelet size but in a manner that is opposite to many other types of nanomaterials. For the fluoromicas, the smaller the platelet size, the less toxicity was observed. The small fluoromica nanoparticles (<200 nm) were internalized by macrophages via scavenger receptors, which was dependent on the protein corona formed in serum. This internalization was associated with apoptosis in RAW cells but not in dTHP-1 cells. The larger particles were not internalized efficiently but mostly decorated the surface of the cells, causing membrane disruption, even in the presence of 80% serum. This work suggests the smaller fluoromica platelets may be safer for use in humans but their propensity to recognize macrophage scavenger receptors also suggests that they will target the reticulo-endoplasmic system in vivo.Keywords: layered silicates, accumulation, phagocytosis, high energy millingTee NZhu YMortimer GMMartin DJMinchin RFDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2363-2375 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tee N Zhu Y Mortimer GM Martin DJ Minchin RF Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
description	Nicolin Tee,1 Yingdong Zhu,2 Gysell M Mortimer,1 Darren J Martin,2 Rodney F Minchin11School of Biomedical Science, University of Queensland, Brisbane, QLD, Australia; 2Australian Institute of Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD, AustraliaAbstract: Polyurethanes are widely used in biomedical devices such as heart valves, pacemaker leads, catheters, vascular devices, and surgical dressings because of their excellent mechanical properties and good biocompatibility. Layered silicate nanoparticles can significantly increase tensile strength and breaking strain of polyurethanes potentially increasing the life span of biomedical devices that suffer from wear in vivo. However, very little is known about how these nanoparticles interact with proteins and cells and how they might exert unwanted effects. A series of fluoromica nanoparticles ranging in platelet size from 90 to over 600 nm in diameter were generated from the same base material ME100 by high energy milling and differential centrifugation. The cytotoxicity of the resulting particles was dependent on platelet size but in a manner that is opposite to many other types of nanomaterials. For the fluoromicas, the smaller the platelet size, the less toxicity was observed. The small fluoromica nanoparticles (<200 nm) were internalized by macrophages via scavenger receptors, which was dependent on the protein corona formed in serum. This internalization was associated with apoptosis in RAW cells but not in dTHP-1 cells. The larger particles were not internalized efficiently but mostly decorated the surface of the cells, causing membrane disruption, even in the presence of 80% serum. This work suggests the smaller fluoromica platelets may be safer for use in humans but their propensity to recognize macrophage scavenger receptors also suggests that they will target the reticulo-endoplasmic system in vivo.Keywords: layered silicates, accumulation, phagocytosis, high energy milling
format	article
author	Tee N Zhu Y Mortimer GM Martin DJ Minchin RF
author_facet	Tee N Zhu Y Mortimer GM Martin DJ Minchin RF
author_sort	Tee N
title	Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
title_short	Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
title_full	Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
title_fullStr	Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
title_full_unstemmed	Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
title_sort	fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/1f414d5ad9aa4e0883e5e405e415775d
work_keys_str_mv	AT teen fluoromicananoparticlecytotoxicityinmacrophagesdecreaseswithsizeandextentofnbspuptake AT zhuy fluoromicananoparticlecytotoxicityinmacrophagesdecreaseswithsizeandextentofnbspuptake AT mortimergm fluoromicananoparticlecytotoxicityinmacrophagesdecreaseswithsizeandextentofnbspuptake AT martindj fluoromicananoparticlecytotoxicityinmacrophagesdecreaseswithsizeandextentofnbspuptake AT minchinrf fluoromicananoparticlecytotoxicityinmacrophagesdecreaseswithsizeandextentofnbspuptake
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Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of&nbsp;uptake

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