Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate

The importance of atmospheric nitrogen-fixing bacteria The study aimed to isolate bacteria from the roots of plants From four areas of the city of Mosul the winter agriculture, season 2020-2021 and using biochemical tests and phenotypic and agricultural characteristics to diagnose bacterial isolates...

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Autores principales:	Samar Alhayale, Mohammed Al-Shakarchi
Formato:	article
Lenguaje:	AR EN
Publicado:	College of Education for Pure Sciences 2021
Materias:	rhizobium,, ,،,؛antibiotics,, ,،,؛heavy material,, ,،,؛pcr,, ,،,؛16s rrna Education L Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/1f470bc39af24ba8a8fb5e4d2f2e4e23
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spelling	oai:doaj.org-article:1f470bc39af24ba8a8fb5e4d2f2e4e232021-12-01T14:54:26ZGenetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate1812-125X2664-253010.33899/edusj.2021.131302.1188https://doaj.org/article/1f470bc39af24ba8a8fb5e4d2f2e4e232021-12-01T00:00:00Zhttps://edusj.mosuljournals.com/article_169834_abf2a9cdb18ce3bfa6ce3acfa860a31c.pdfhttps://doaj.org/toc/1812-125Xhttps://doaj.org/toc/2664-2530The importance of atmospheric nitrogen-fixing bacteria The study aimed to isolate bacteria from the roots of plants From four areas of the city of Mosul the winter agriculture, season 2020-2021 and using biochemical tests and phenotypic and agricultural characteristics to diagnose bacterial isolates and study sensitivity and resistance of bacterial isolates to ten antibiotics. if the results showed that the highest percentage of resistance was to the two antibiotics, Trimethoprim, and streptomycin, at 100%, while The least resistance was to the two antivirals Gentamycin and Rifampicin by 20%. In addition to Studying The sensitivity and resistance of bacterial isolates to four heavy Metals, namely Mercury chloride (HgCl), Cobalt chloride (CoCl), Cadmium Chloride (CdCl), and nickel chloride (NiCl). The resistance was high with Cadmium chloride salt (CdCl) and the lowest salt Mercury Chloride (HgCl). PCR techniques were used to diagnose samples based on 16S rRNA sequence analysis and comparison of sequences resulting from DNA amplification with standard isolates within NCBI to detect new bacterial isolatesSamar AlhayaleMohammed Al-ShakarchiCollege of Education for Pure Sciencesarticlerhizobium,,,،,؛antibiotics,,,،,؛heavy material,,,،,؛pcr,,,،,؛16s rrnaEducationLScience (General)Q1-390ARENمجلة التربية والعلم, Vol 30, Iss 5, Pp 163-177 (2021)
institution	DOAJ
collection	DOAJ
language	AR EN
topic	rhizobium,, ,،,؛antibiotics,, ,،,؛heavy material,, ,،,؛pcr,, ,،,؛16s rrna Education L Science (General) Q1-390
spellingShingle	rhizobium,, ,،,؛antibiotics,, ,،,؛heavy material,, ,،,؛pcr,, ,،,؛16s rrna Education L Science (General) Q1-390 Samar Alhayale Mohammed Al-Shakarchi Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate
description	The importance of atmospheric nitrogen-fixing bacteria The study aimed to isolate bacteria from the roots of plants From four areas of the city of Mosul the winter agriculture, season 2020-2021 and using biochemical tests and phenotypic and agricultural characteristics to diagnose bacterial isolates and study sensitivity and resistance of bacterial isolates to ten antibiotics. if the results showed that the highest percentage of resistance was to the two antibiotics, Trimethoprim, and streptomycin, at 100%, while The least resistance was to the two antivirals Gentamycin and Rifampicin by 20%. In addition to Studying The sensitivity and resistance of bacterial isolates to four heavy Metals, namely Mercury chloride (HgCl), Cobalt chloride (CoCl), Cadmium Chloride (CdCl), and nickel chloride (NiCl). The resistance was high with Cadmium chloride salt (CdCl) and the lowest salt Mercury Chloride (HgCl). PCR techniques were used to diagnose samples based on 16S rRNA sequence analysis and comparison of sequences resulting from DNA amplification with standard isolates within NCBI to detect new bacterial isolates
format	article
author	Samar Alhayale Mohammed Al-Shakarchi
author_facet	Samar Alhayale Mohammed Al-Shakarchi
author_sort	Samar Alhayale
title	Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate
title_short	Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate
title_full	Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate
title_fullStr	Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate
title_full_unstemmed	Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate
title_sort	genetic diagnosis of some root nodule bacteria isolated from leguminous plants in nineveh governorate
publisher	College of Education for Pure Sciences
publishDate	2021
url	https://doaj.org/article/1f470bc39af24ba8a8fb5e4d2f2e4e23
work_keys_str_mv	AT samaralhayale geneticdiagnosisofsomerootnodulebacteriaisolatedfromleguminousplantsinninevehgovernorate AT mohammedalshakarchi geneticdiagnosisofsomerootnodulebacteriaisolatedfromleguminousplantsinninevehgovernorate
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Genetic diagnosis of some root nodule bacteria isolated from leguminous Plants in Nineveh Governorate

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