Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.

NAC (NAM, ATAF1-2, and CUC2) proteins constitute one of the largest families of plant-specific transcription factors and have been shown to be involved in diverse plant processes including plant growth, development, and stress-tolerance. In this study, a stress-responsive NAC gene, EcNAC1, was isola...

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Autores principales:	Venkategowda Ramegowda, Muthappa Senthil-Kumar, Karaba N Nataraja, Malireddy K Reddy, Kirankumar S Mysore, Makarla Udayakumar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f4862cfa5f545b9bd4475671598ede0
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spelling	oai:doaj.org-article:1f4862cfa5f545b9bd4475671598ede02021-11-18T07:12:45ZExpression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.1932-620310.1371/journal.pone.0040397https://doaj.org/article/1f4862cfa5f545b9bd4475671598ede02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22808152/?tool=EBIhttps://doaj.org/toc/1932-6203NAC (NAM, ATAF1-2, and CUC2) proteins constitute one of the largest families of plant-specific transcription factors and have been shown to be involved in diverse plant processes including plant growth, development, and stress-tolerance. In this study, a stress-responsive NAC gene, EcNAC1, was isolated from the subtracted stress cDNA library generated from a drought adapted crop, finger millet, and characterized for its role in stress-tolerance. The expression analysis showed that EcNAC1 was highly induced during water-deficit and salt stress. EcNAC1 shares high amino acid similarity with rice genes that have been phylogenetically classified into stress-related NAC genes. Our results demonstrated that tobacco transgenic plants expressing EcNAC1 exhibit tolerance to various abiotic stresses like simulated osmotic stress, by polyethylene glycol (PEG) and mannitol, and salinity stress. The transgenic plants also showed enhanced tolerance to methyl-viologen (MV) induced oxidative stress. Reduced levels of reactive oxygen species (ROS) and ROS-induced damage were noticed in pot grown transgenic lines under water-deficit and natural high light conditions. Root growth under stress and recovery growth after stress alleviation was more in transgenic plants. Many stress-responsive genes were found to be up-regulated in transgenic lines expressing EcNAC1. Our results suggest that EcNAC1 overexpression confers tolerance against abiotic stress in susceptible species, tobacco.Venkategowda RamegowdaMuthappa Senthil-KumarKaraba N NatarajaMalireddy K ReddyKirankumar S MysoreMakarla UdayakumarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40397 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Venkategowda Ramegowda Muthappa Senthil-Kumar Karaba N Nataraja Malireddy K Reddy Kirankumar S Mysore Makarla Udayakumar Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.
description	NAC (NAM, ATAF1-2, and CUC2) proteins constitute one of the largest families of plant-specific transcription factors and have been shown to be involved in diverse plant processes including plant growth, development, and stress-tolerance. In this study, a stress-responsive NAC gene, EcNAC1, was isolated from the subtracted stress cDNA library generated from a drought adapted crop, finger millet, and characterized for its role in stress-tolerance. The expression analysis showed that EcNAC1 was highly induced during water-deficit and salt stress. EcNAC1 shares high amino acid similarity with rice genes that have been phylogenetically classified into stress-related NAC genes. Our results demonstrated that tobacco transgenic plants expressing EcNAC1 exhibit tolerance to various abiotic stresses like simulated osmotic stress, by polyethylene glycol (PEG) and mannitol, and salinity stress. The transgenic plants also showed enhanced tolerance to methyl-viologen (MV) induced oxidative stress. Reduced levels of reactive oxygen species (ROS) and ROS-induced damage were noticed in pot grown transgenic lines under water-deficit and natural high light conditions. Root growth under stress and recovery growth after stress alleviation was more in transgenic plants. Many stress-responsive genes were found to be up-regulated in transgenic lines expressing EcNAC1. Our results suggest that EcNAC1 overexpression confers tolerance against abiotic stress in susceptible species, tobacco.
format	article
author	Venkategowda Ramegowda Muthappa Senthil-Kumar Karaba N Nataraja Malireddy K Reddy Kirankumar S Mysore Makarla Udayakumar
author_facet	Venkategowda Ramegowda Muthappa Senthil-Kumar Karaba N Nataraja Malireddy K Reddy Kirankumar S Mysore Makarla Udayakumar
author_sort	Venkategowda Ramegowda
title	Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.
title_short	Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.
title_full	Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.
title_fullStr	Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.
title_full_unstemmed	Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.
title_sort	expression of a finger millet transcription factor, ecnac1, in tobacco confers abiotic stress-tolerance.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1f4862cfa5f545b9bd4475671598ede0
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Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance.

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