A shared transcriptional code orchestrates temporal patterning of the central nervous system.
The molecular mechanisms that produce the full array of neuronal subtypes in the vertebrate nervous system are incompletely understood. Here, we provide evidence of a global temporal patterning program comprising sets of transcription factors that stratifies neurons based on the developmental time a...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1f4bade4a91e47e8a61775cad7bde81e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:1f4bade4a91e47e8a61775cad7bde81e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:1f4bade4a91e47e8a61775cad7bde81e2021-12-02T19:54:42ZA shared transcriptional code orchestrates temporal patterning of the central nervous system.1544-91731545-788510.1371/journal.pbio.3001450https://doaj.org/article/1f4bade4a91e47e8a61775cad7bde81e2021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.3001450https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The molecular mechanisms that produce the full array of neuronal subtypes in the vertebrate nervous system are incompletely understood. Here, we provide evidence of a global temporal patterning program comprising sets of transcription factors that stratifies neurons based on the developmental time at which they are generated. This transcriptional code acts throughout the central nervous system, in parallel to spatial patterning, thereby increasing the diversity of neurons generated along the neuraxis. We further demonstrate that this temporal program operates in stem cell-derived neurons and is under the control of the TGFβ signaling pathway. Targeted perturbation of components of the temporal program, Nfia and Nfib, reveals their functional requirement for the generation of late-born neuronal subtypes. Together, our results provide evidence for the existence of a previously unappreciated global temporal transcriptional program of neuronal subtype identity and suggest that the integration of spatial and temporal patterning mechanisms diversifies and organizes neuronal subtypes in the vertebrate nervous system.Andreas SagnerIsabel ZhangThomas WatsonJorge LazaroManuela MelchiondaJames BriscoePublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 19, Iss 11, p e3001450 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Andreas Sagner Isabel Zhang Thomas Watson Jorge Lazaro Manuela Melchionda James Briscoe A shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| description |
The molecular mechanisms that produce the full array of neuronal subtypes in the vertebrate nervous system are incompletely understood. Here, we provide evidence of a global temporal patterning program comprising sets of transcription factors that stratifies neurons based on the developmental time at which they are generated. This transcriptional code acts throughout the central nervous system, in parallel to spatial patterning, thereby increasing the diversity of neurons generated along the neuraxis. We further demonstrate that this temporal program operates in stem cell-derived neurons and is under the control of the TGFβ signaling pathway. Targeted perturbation of components of the temporal program, Nfia and Nfib, reveals their functional requirement for the generation of late-born neuronal subtypes. Together, our results provide evidence for the existence of a previously unappreciated global temporal transcriptional program of neuronal subtype identity and suggest that the integration of spatial and temporal patterning mechanisms diversifies and organizes neuronal subtypes in the vertebrate nervous system. |
| format |
article |
| author |
Andreas Sagner Isabel Zhang Thomas Watson Jorge Lazaro Manuela Melchionda James Briscoe |
| author_facet |
Andreas Sagner Isabel Zhang Thomas Watson Jorge Lazaro Manuela Melchionda James Briscoe |
| author_sort |
Andreas Sagner |
| title |
A shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| title_short |
A shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| title_full |
A shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| title_fullStr |
A shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| title_full_unstemmed |
A shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| title_sort |
shared transcriptional code orchestrates temporal patterning of the central nervous system. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/1f4bade4a91e47e8a61775cad7bde81e |
| work_keys_str_mv |
AT andreassagner asharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT isabelzhang asharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT thomaswatson asharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT jorgelazaro asharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT manuelamelchionda asharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT jamesbriscoe asharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT andreassagner sharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT isabelzhang sharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT thomaswatson sharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT jorgelazaro sharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT manuelamelchionda sharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem AT jamesbriscoe sharedtranscriptionalcodeorchestratestemporalpatterningofthecentralnervoussystem |
| _version_ |
1718375887546613760 |