Finer-Scale Phylosymbiosis: Insights from Insect Viromes

ABSTRACT Phylosymbiosis was recently proposed to describe the eco-evolutionary pattern whereby the ecological relatedness (e.g., beta diversity relationships) of host-associated microbial communities parallels the phylogeny of the host species. Representing the most abundant biological entities on t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Brittany A. Leigh, Sarah R. Bordenstein, Andrew W. Brooks, Aram Mikaelyan, Seth R. Bordenstein
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Nasonia Proteus Providencia Morganella bacteriophage microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1f4c93b83ee44438bb4da58519048caf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1f4c93b83ee44438bb4da58519048caf
record_format	dspace
spelling	oai:doaj.org-article:1f4c93b83ee44438bb4da58519048caf2021-12-02T19:46:17ZFiner-Scale Phylosymbiosis: Insights from Insect Viromes10.1128/mSystems.00131-182379-5077https://doaj.org/article/1f4c93b83ee44438bb4da58519048caf2018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00131-18https://doaj.org/toc/2379-5077ABSTRACT Phylosymbiosis was recently proposed to describe the eco-evolutionary pattern whereby the ecological relatedness (e.g., beta diversity relationships) of host-associated microbial communities parallels the phylogeny of the host species. Representing the most abundant biological entities on the planet and common members of the animal-associated microbiome, viruses can be influential members of host-associated microbial communities that may recapitulate, reinforce, or ablate phylosymbiosis. Here we sequence the metagenomes of purified viral communities from three different parasitic wasp Nasonia species, one cytonuclear introgression line of Nasonia, and the flour moth outgroup Ephestia kuehniella. Results demonstrate complete phylosymbiosis between the viral metagenome and insect phylogeny. Across all Nasonia contigs, 69% of the genes in the viral metagenomes are either new to the databases or uncharacterized, yet over 99% of the contigs have at least one gene with similarity to a known sequence. The core Nasonia virome spans 21% of the total contigs, and the majority of that core is likely derived from induced prophages residing in the genomes of common Nasonia-associated bacterial genera: Proteus, Providencia, and Morganella. We also assemble the first complete viral particle genomes from Nasonia-associated gut bacteria. Taken together, results reveal the first complete evidence for phylosymbiosis in viral metagenomes, new genome sequences of viral particles from Nasonia-associated gut bacteria, and a large set of novel or uncharacterized genes in the Nasonia virome. This work suggests that phylosymbiosis at the host-microbiome level will likely extend to the host-virome level in other systems as well. IMPORTANCE Viruses are the most abundant biological entity on the planet and interact with microbial communities with which they associate. The virome of animals is often dominated by bacterial viruses, known as bacteriophages or phages, which can (re)structure bacterial communities potentially vital to the animal host. Beta diversity relationships of animal-associated bacterial communities in laboratory and wild populations frequently parallel animal phylogenetic relationships, a pattern termed phylosymbiosis. However, little is known about whether viral communities also exhibit this eco-evolutionary pattern. Metagenomics of purified viruses from recently diverged species of Nasonia parasitoid wasps reared in the lab indicates for the first time that the community relationships of the virome can also exhibit complete phylosymbiosis. Therefore, viruses, particularly bacteriophages here, may also be influenced by animal evolutionary changes either directly or indirectly through the tripartite interactions among hosts, bacteria, and phage communities. Moreover, we report several new bacteriophage genomes from the common gut bacteria in Nasonia.Brittany A. LeighSarah R. BordensteinAndrew W. BrooksAram MikaelyanSeth R. BordensteinAmerican Society for MicrobiologyarticleNasoniaProteusProvidenciaMorganellabacteriophagemicrobiomeMicrobiologyQR1-502ENmSystems, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nasonia Proteus Providencia Morganella bacteriophage microbiome Microbiology QR1-502
spellingShingle	Nasonia Proteus Providencia Morganella bacteriophage microbiome Microbiology QR1-502 Brittany A. Leigh Sarah R. Bordenstein Andrew W. Brooks Aram Mikaelyan Seth R. Bordenstein Finer-Scale Phylosymbiosis: Insights from Insect Viromes
description	ABSTRACT Phylosymbiosis was recently proposed to describe the eco-evolutionary pattern whereby the ecological relatedness (e.g., beta diversity relationships) of host-associated microbial communities parallels the phylogeny of the host species. Representing the most abundant biological entities on the planet and common members of the animal-associated microbiome, viruses can be influential members of host-associated microbial communities that may recapitulate, reinforce, or ablate phylosymbiosis. Here we sequence the metagenomes of purified viral communities from three different parasitic wasp Nasonia species, one cytonuclear introgression line of Nasonia, and the flour moth outgroup Ephestia kuehniella. Results demonstrate complete phylosymbiosis between the viral metagenome and insect phylogeny. Across all Nasonia contigs, 69% of the genes in the viral metagenomes are either new to the databases or uncharacterized, yet over 99% of the contigs have at least one gene with similarity to a known sequence. The core Nasonia virome spans 21% of the total contigs, and the majority of that core is likely derived from induced prophages residing in the genomes of common Nasonia-associated bacterial genera: Proteus, Providencia, and Morganella. We also assemble the first complete viral particle genomes from Nasonia-associated gut bacteria. Taken together, results reveal the first complete evidence for phylosymbiosis in viral metagenomes, new genome sequences of viral particles from Nasonia-associated gut bacteria, and a large set of novel or uncharacterized genes in the Nasonia virome. This work suggests that phylosymbiosis at the host-microbiome level will likely extend to the host-virome level in other systems as well. IMPORTANCE Viruses are the most abundant biological entity on the planet and interact with microbial communities with which they associate. The virome of animals is often dominated by bacterial viruses, known as bacteriophages or phages, which can (re)structure bacterial communities potentially vital to the animal host. Beta diversity relationships of animal-associated bacterial communities in laboratory and wild populations frequently parallel animal phylogenetic relationships, a pattern termed phylosymbiosis. However, little is known about whether viral communities also exhibit this eco-evolutionary pattern. Metagenomics of purified viruses from recently diverged species of Nasonia parasitoid wasps reared in the lab indicates for the first time that the community relationships of the virome can also exhibit complete phylosymbiosis. Therefore, viruses, particularly bacteriophages here, may also be influenced by animal evolutionary changes either directly or indirectly through the tripartite interactions among hosts, bacteria, and phage communities. Moreover, we report several new bacteriophage genomes from the common gut bacteria in Nasonia.
format	article
author	Brittany A. Leigh Sarah R. Bordenstein Andrew W. Brooks Aram Mikaelyan Seth R. Bordenstein
author_facet	Brittany A. Leigh Sarah R. Bordenstein Andrew W. Brooks Aram Mikaelyan Seth R. Bordenstein
author_sort	Brittany A. Leigh
title	Finer-Scale Phylosymbiosis: Insights from Insect Viromes
title_short	Finer-Scale Phylosymbiosis: Insights from Insect Viromes
title_full	Finer-Scale Phylosymbiosis: Insights from Insect Viromes
title_fullStr	Finer-Scale Phylosymbiosis: Insights from Insect Viromes
title_full_unstemmed	Finer-Scale Phylosymbiosis: Insights from Insect Viromes
title_sort	finer-scale phylosymbiosis: insights from insect viromes
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/1f4c93b83ee44438bb4da58519048caf
work_keys_str_mv	AT brittanyaleigh finerscalephylosymbiosisinsightsfrominsectviromes AT sarahrbordenstein finerscalephylosymbiosisinsightsfrominsectviromes AT andrewwbrooks finerscalephylosymbiosisinsightsfrominsectviromes AT arammikaelyan finerscalephylosymbiosisinsightsfrominsectviromes AT sethrbordenstein finerscalephylosymbiosisinsightsfrominsectviromes
_version_	1718376012537921536

Finer-Scale Phylosymbiosis: Insights from Insect Viromes

Ejemplares similares