LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages

Abstract Long noncoding RNAs (lncRNAs) are emerging as major regulators of a variety of cell signaling processes. Many lncRNAs are expressed in immune cells and appear to play critical roles in the regulation of immune response. Here, we have investigated the potential role of a well-known lncRNA, H...

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Autores principales:	Monira Obaid, S. M. Nashir Udden, Paromita Deb, Nadine Shihabeddin, Md. Hasan Zaki, Subhrangsu S. Mandal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	HOX Transcript Antisense Intergenic RNA (HOTAIR) lncRNAs HOTAIR Expression Enhancer Of Zeste Homolog 2 (EZH2) Nuclear Factor Of Activated T (NFAT) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f55d91dc3db457aaef4b4a662414db3
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spelling	oai:doaj.org-article:1f55d91dc3db457aaef4b4a662414db32021-12-02T15:07:45ZLncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages10.1038/s41598-018-33722-22045-2322https://doaj.org/article/1f55d91dc3db457aaef4b4a662414db32018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33722-2https://doaj.org/toc/2045-2322Abstract Long noncoding RNAs (lncRNAs) are emerging as major regulators of a variety of cell signaling processes. Many lncRNAs are expressed in immune cells and appear to play critical roles in the regulation of immune response. Here, we have investigated the potential role of a well-known lncRNA, HOTAIR, in inflammatory and immune response. Our studies demonstrate that HOTAIR expression is induced in immune cells (macrophages) upon treatment with lipopolysaccharide (LPS). Knockdown of HOTAIR reduces NF-κB-mediated inflammatory gene and cytokine expression in macrophages. Inhibition of NF-κB resulted in down-regulation of LPS-induced expression of HOTAIR as well as IL-6 and iNOS expression. We further demonstrated that HOTAIR regulates activation of NF-κB and its target genes (IL-6 and iNOS) expression via facilitating the degradation of IκBα. HOTAIR knockdown reduces the expression of NF-κB target gene expression via inhibiting the recruitment of NF-κB and associated cofactors at the target gene promoters. Taken together, our findings suggest that HOTAIR is a critical player in NF-κB activation in macrophages suggesting its potential functions in inflammatory and immune response.Monira ObaidS. M. Nashir UddenParomita DebNadine ShihabeddinMd. Hasan ZakiSubhrangsu S. MandalNature PortfolioarticleHOX Transcript Antisense Intergenic RNA (HOTAIR)lncRNAsHOTAIR ExpressionEnhancer Of Zeste Homolog 2 (EZH2)Nuclear Factor Of Activated T (NFAT)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-18 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	HOX Transcript Antisense Intergenic RNA (HOTAIR) lncRNAs HOTAIR Expression Enhancer Of Zeste Homolog 2 (EZH2) Nuclear Factor Of Activated T (NFAT) Medicine R Science Q
spellingShingle	HOX Transcript Antisense Intergenic RNA (HOTAIR) lncRNAs HOTAIR Expression Enhancer Of Zeste Homolog 2 (EZH2) Nuclear Factor Of Activated T (NFAT) Medicine R Science Q Monira Obaid S. M. Nashir Udden Paromita Deb Nadine Shihabeddin Md. Hasan Zaki Subhrangsu S. Mandal LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
description	Abstract Long noncoding RNAs (lncRNAs) are emerging as major regulators of a variety of cell signaling processes. Many lncRNAs are expressed in immune cells and appear to play critical roles in the regulation of immune response. Here, we have investigated the potential role of a well-known lncRNA, HOTAIR, in inflammatory and immune response. Our studies demonstrate that HOTAIR expression is induced in immune cells (macrophages) upon treatment with lipopolysaccharide (LPS). Knockdown of HOTAIR reduces NF-κB-mediated inflammatory gene and cytokine expression in macrophages. Inhibition of NF-κB resulted in down-regulation of LPS-induced expression of HOTAIR as well as IL-6 and iNOS expression. We further demonstrated that HOTAIR regulates activation of NF-κB and its target genes (IL-6 and iNOS) expression via facilitating the degradation of IκBα. HOTAIR knockdown reduces the expression of NF-κB target gene expression via inhibiting the recruitment of NF-κB and associated cofactors at the target gene promoters. Taken together, our findings suggest that HOTAIR is a critical player in NF-κB activation in macrophages suggesting its potential functions in inflammatory and immune response.
format	article
author	Monira Obaid S. M. Nashir Udden Paromita Deb Nadine Shihabeddin Md. Hasan Zaki Subhrangsu S. Mandal
author_facet	Monira Obaid S. M. Nashir Udden Paromita Deb Nadine Shihabeddin Md. Hasan Zaki Subhrangsu S. Mandal
author_sort	Monira Obaid
title	LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
title_short	LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
title_full	LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
title_fullStr	LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
title_full_unstemmed	LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
title_sort	lncrna hotair regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1f55d91dc3db457aaef4b4a662414db3
work_keys_str_mv	AT moniraobaid lncrnahotairregulateslipopolysaccharideinducedcytokineexpressionandinflammatoryresponseinmacrophages AT smnashirudden lncrnahotairregulateslipopolysaccharideinducedcytokineexpressionandinflammatoryresponseinmacrophages AT paromitadeb lncrnahotairregulateslipopolysaccharideinducedcytokineexpressionandinflammatoryresponseinmacrophages AT nadineshihabeddin lncrnahotairregulateslipopolysaccharideinducedcytokineexpressionandinflammatoryresponseinmacrophages AT mdhasanzaki lncrnahotairregulateslipopolysaccharideinducedcytokineexpressionandinflammatoryresponseinmacrophages AT subhrangsusmandal lncrnahotairregulateslipopolysaccharideinducedcytokineexpressionandinflammatoryresponseinmacrophages
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LncRNA HOTAIR regulates lipopolysaccharide-induced cytokine expression and inflammatory response in macrophages

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