A new paradigm for leprosy diagnosis based on host gene expression.

Transcriptional profiling is a powerful tool to investigate and detect human diseases. In this study, we used bulk RNA-sequencing (RNA-Seq) to compare the transcriptomes in skin lesions of leprosy patients or controls affected by other dermal conditions such as granuloma annulare, a confounder for p...

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Autores principales:	Thyago Leal-Calvo, Charlotte Avanzi, Mayara Abud Mendes, Andrej Benjak, Philippe Busso, Roberta Olmo Pinheiro, Euzenir Nunes Sarno, Stewart Thomas Cole, Milton Ozório Moraes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1f59cb6727cc4942a3c60c07f6f4ad26
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spelling	oai:doaj.org-article:1f59cb6727cc4942a3c60c07f6f4ad262021-12-02T19:59:59ZA new paradigm for leprosy diagnosis based on host gene expression.1553-73661553-737410.1371/journal.ppat.1009972https://doaj.org/article/1f59cb6727cc4942a3c60c07f6f4ad262021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009972https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Transcriptional profiling is a powerful tool to investigate and detect human diseases. In this study, we used bulk RNA-sequencing (RNA-Seq) to compare the transcriptomes in skin lesions of leprosy patients or controls affected by other dermal conditions such as granuloma annulare, a confounder for paucibacillary leprosy. We identified five genes capable of accurately distinguishing multibacillary and paucibacillary leprosy from other skin conditions. Indoleamine 2,3-dioxygenase 1 (IDO1) expression alone was highly discriminatory, followed by TLR10, BLK, CD38, and SLAMF7, whereas the HS3ST2 and CD40LG mRNA separated multi- and paucibacillary leprosy. Finally, from the main differentially expressed genes (DEG) and enriched pathways, we conclude that paucibacillary disease is characterized by epithelioid transformation and granuloma formation, with an exacerbated cellular immune response, while multibacillary leprosy features epithelial-mesenchymal transition with phagocytic and lipid biogenesis patterns in the skin. These findings will help catalyze the development of better diagnostic tools and potential host-based therapeutic interventions. Finally, our data may help elucidate host-pathogen interplay driving disease clinical manifestations.Thyago Leal-CalvoCharlotte AvanziMayara Abud MendesAndrej BenjakPhilippe BussoRoberta Olmo PinheiroEuzenir Nunes SarnoStewart Thomas ColeMilton Ozório MoraesPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 10, p e1009972 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Thyago Leal-Calvo Charlotte Avanzi Mayara Abud Mendes Andrej Benjak Philippe Busso Roberta Olmo Pinheiro Euzenir Nunes Sarno Stewart Thomas Cole Milton Ozório Moraes A new paradigm for leprosy diagnosis based on host gene expression.
description	Transcriptional profiling is a powerful tool to investigate and detect human diseases. In this study, we used bulk RNA-sequencing (RNA-Seq) to compare the transcriptomes in skin lesions of leprosy patients or controls affected by other dermal conditions such as granuloma annulare, a confounder for paucibacillary leprosy. We identified five genes capable of accurately distinguishing multibacillary and paucibacillary leprosy from other skin conditions. Indoleamine 2,3-dioxygenase 1 (IDO1) expression alone was highly discriminatory, followed by TLR10, BLK, CD38, and SLAMF7, whereas the HS3ST2 and CD40LG mRNA separated multi- and paucibacillary leprosy. Finally, from the main differentially expressed genes (DEG) and enriched pathways, we conclude that paucibacillary disease is characterized by epithelioid transformation and granuloma formation, with an exacerbated cellular immune response, while multibacillary leprosy features epithelial-mesenchymal transition with phagocytic and lipid biogenesis patterns in the skin. These findings will help catalyze the development of better diagnostic tools and potential host-based therapeutic interventions. Finally, our data may help elucidate host-pathogen interplay driving disease clinical manifestations.
format	article
author	Thyago Leal-Calvo Charlotte Avanzi Mayara Abud Mendes Andrej Benjak Philippe Busso Roberta Olmo Pinheiro Euzenir Nunes Sarno Stewart Thomas Cole Milton Ozório Moraes
author_facet	Thyago Leal-Calvo Charlotte Avanzi Mayara Abud Mendes Andrej Benjak Philippe Busso Roberta Olmo Pinheiro Euzenir Nunes Sarno Stewart Thomas Cole Milton Ozório Moraes
author_sort	Thyago Leal-Calvo
title	A new paradigm for leprosy diagnosis based on host gene expression.
title_short	A new paradigm for leprosy diagnosis based on host gene expression.
title_full	A new paradigm for leprosy diagnosis based on host gene expression.
title_fullStr	A new paradigm for leprosy diagnosis based on host gene expression.
title_full_unstemmed	A new paradigm for leprosy diagnosis based on host gene expression.
title_sort	new paradigm for leprosy diagnosis based on host gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1f59cb6727cc4942a3c60c07f6f4ad26
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A new paradigm for leprosy diagnosis based on host gene expression.

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