Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress

In scallops, gonad production is highly demanding energetically, and reproduction usually occurs during spring-summer, a period of strong environmental changes. The synthesis of heat-shock proteins (HSPs) is a major mechanism of stress tolerance in animals, including scallops, and HSP expression con...

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Autores principales:	K Brokordt, H Pérez, C Herrera, A Gallardo
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2015
Materias:	Biology (General) QH301-705.5 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1f5bb8e00d3c4f659a3c0f8be2de1952
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spelling	oai:doaj.org-article:1f5bb8e00d3c4f659a3c0f8be2de19522021-11-18T09:21:04ZReproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress1864-77821864-779010.3354/ab00626https://doaj.org/article/1f5bb8e00d3c4f659a3c0f8be2de19522015-04-01T00:00:00Zhttps://www.int-res.com/abstracts/ab/v23/n3/p265-274/https://doaj.org/toc/1864-7782https://doaj.org/toc/1864-7790In scallops, gonad production is highly demanding energetically, and reproduction usually occurs during spring-summer, a period of strong environmental changes. The synthesis of heat-shock proteins (HSPs) is a major mechanism of stress tolerance in animals, including scallops, and HSP expression contributes considerably to cellular energy demand. Therefore, reproductive investment may limit the availability of energy (in terms of ATP) for the expression of HSP in organisms exposed to environmental stress. We evaluated the stress response capacity of adult Argopecten purpuratus scallops to high temperature and hypoxia. Stress response capacity was assessed through gene expression (for temperature stress) and protein induction of 70 kD HSP at 3 reproductive stages: immature, mature and spawned. We also evaluated the effect of reproductive status on the cellular ATP provisioning capacity through citrate synthase activity. Immature scallops exposed to thermal stress showed 1.3- and 1.5-fold increases in hsp70 mRNA and HSP70 protein levels, respectively, and those exposed to hypoxia doubled their level of HSP70 compared to non-stressed immature scallops. However, following gonad maturation and spawning, hsp70 mRNA increased by only 0.49- and 0.65-fold, respectively, after thermal stress and HSP70 protein levels of scallops exposed to thermal and hypoxia stressors did not differ from those of non-stressed animals. In parallel, citrate synthase showed its highest level in immature scallops, declined with gonad maturation, and was lowest in spawned scallops. These results suggest that reproductive investment reduces the stress response capacity of A. purpuratus and that mature and spawned scallops could be more vulnerable to environmental stressors than immature individuals.K BrokordtH PérezC HerreraA GallardoInter-ResearcharticleBiology (General)QH301-705.5MicrobiologyQR1-502ENAquatic Biology, Vol 23, Iss 3, Pp 265-274 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5 Microbiology QR1-502
spellingShingle	Biology (General) QH301-705.5 Microbiology QR1-502 K Brokordt H Pérez C Herrera A Gallardo Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress
description	In scallops, gonad production is highly demanding energetically, and reproduction usually occurs during spring-summer, a period of strong environmental changes. The synthesis of heat-shock proteins (HSPs) is a major mechanism of stress tolerance in animals, including scallops, and HSP expression contributes considerably to cellular energy demand. Therefore, reproductive investment may limit the availability of energy (in terms of ATP) for the expression of HSP in organisms exposed to environmental stress. We evaluated the stress response capacity of adult Argopecten purpuratus scallops to high temperature and hypoxia. Stress response capacity was assessed through gene expression (for temperature stress) and protein induction of 70 kD HSP at 3 reproductive stages: immature, mature and spawned. We also evaluated the effect of reproductive status on the cellular ATP provisioning capacity through citrate synthase activity. Immature scallops exposed to thermal stress showed 1.3- and 1.5-fold increases in hsp70 mRNA and HSP70 protein levels, respectively, and those exposed to hypoxia doubled their level of HSP70 compared to non-stressed immature scallops. However, following gonad maturation and spawning, hsp70 mRNA increased by only 0.49- and 0.65-fold, respectively, after thermal stress and HSP70 protein levels of scallops exposed to thermal and hypoxia stressors did not differ from those of non-stressed animals. In parallel, citrate synthase showed its highest level in immature scallops, declined with gonad maturation, and was lowest in spawned scallops. These results suggest that reproductive investment reduces the stress response capacity of A. purpuratus and that mature and spawned scallops could be more vulnerable to environmental stressors than immature individuals.
format	article
author	K Brokordt H Pérez C Herrera A Gallardo
author_facet	K Brokordt H Pérez C Herrera A Gallardo
author_sort	K Brokordt
title	Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress
title_short	Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress
title_full	Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress
title_fullStr	Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress
title_full_unstemmed	Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress
title_sort	reproduction reduces hsp70 expression capacity in argopecten purpuratus scallops subject to hypoxia and heat stress
publisher	Inter-Research
publishDate	2015
url	https://doaj.org/article/1f5bb8e00d3c4f659a3c0f8be2de1952
work_keys_str_mv	AT kbrokordt reproductionreduceshsp70expressioncapacityinargopectenpurpuratusscallopssubjecttohypoxiaandheatstress AT hperez reproductionreduceshsp70expressioncapacityinargopectenpurpuratusscallopssubjecttohypoxiaandheatstress AT cherrera reproductionreduceshsp70expressioncapacityinargopectenpurpuratusscallopssubjecttohypoxiaandheatstress AT agallardo reproductionreduceshsp70expressioncapacityinargopectenpurpuratusscallopssubjecttohypoxiaandheatstress
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Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress

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