Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.

Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrew B Burns, Corinna Doris, Kevin Vehar, Vinit Saxena, Cameron Bardliving, Parviz A Shamlou, M Ian Phillips
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f5fd361eddf44a0a47410ab39d150ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1f5fd361eddf44a0a47410ab39d150ec
record_format	dspace
spelling	oai:doaj.org-article:1f5fd361eddf44a0a47410ab39d150ec2021-12-02T20:10:35ZNovel low shear 3D bioreactor for high purity mesenchymal stem cell production.1932-620310.1371/journal.pone.0252575https://doaj.org/article/1f5fd361eddf44a0a47410ab39d150ec2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252575https://doaj.org/toc/1932-6203Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a robust method of scaled bioreactor culture must be designed to supply the need for high purity, high density hMSC yields. Here we test a scaled down model of a novel bioreactor consisting of an unsubmerged 3D printed Polylactic Acid (PLA) lattice matrix wetted by culture media. The growth matrix is uniform, replicable, and biocompatible, enabling homogenous cell culture in three dimensions. The goal of this study was to prove that hMSCs would culture well in this novel bioreactor design. The system tested resulted in comparable stem cell yields to other cell culture systems using bone marrow derived hMSCs, while maintaining viability (96.54% ±2.82), high purity (>98% expression of combined positive markers), and differentiation potential.Andrew B BurnsCorinna DorisKevin VeharVinit SaxenaCameron BardlivingParviz A ShamlouM Ian PhillipsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252575 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrew B Burns Corinna Doris Kevin Vehar Vinit Saxena Cameron Bardliving Parviz A Shamlou M Ian Phillips Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.
description	Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a robust method of scaled bioreactor culture must be designed to supply the need for high purity, high density hMSC yields. Here we test a scaled down model of a novel bioreactor consisting of an unsubmerged 3D printed Polylactic Acid (PLA) lattice matrix wetted by culture media. The growth matrix is uniform, replicable, and biocompatible, enabling homogenous cell culture in three dimensions. The goal of this study was to prove that hMSCs would culture well in this novel bioreactor design. The system tested resulted in comparable stem cell yields to other cell culture systems using bone marrow derived hMSCs, while maintaining viability (96.54% ±2.82), high purity (>98% expression of combined positive markers), and differentiation potential.
format	article
author	Andrew B Burns Corinna Doris Kevin Vehar Vinit Saxena Cameron Bardliving Parviz A Shamlou M Ian Phillips
author_facet	Andrew B Burns Corinna Doris Kevin Vehar Vinit Saxena Cameron Bardliving Parviz A Shamlou M Ian Phillips
author_sort	Andrew B Burns
title	Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.
title_short	Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.
title_full	Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.
title_fullStr	Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.
title_full_unstemmed	Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.
title_sort	novel low shear 3d bioreactor for high purity mesenchymal stem cell production.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1f5fd361eddf44a0a47410ab39d150ec
work_keys_str_mv	AT andrewbburns novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction AT corinnadoris novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction AT kevinvehar novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction AT vinitsaxena novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction AT cameronbardliving novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction AT parvizashamlou novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction AT mianphillips novellowshear3dbioreactorforhighpuritymesenchymalstemcellproduction
_version_	1718374988007866368

Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.

Ejemplares similares