The mutational impact of culturing human pluripotent and adult stem cells

Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lower...

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Autores principales:	Ewart Kuijk, Myrthe Jager, Bastiaan van der Roest, Mauro D. Locati, Arne Van Hoeck, Jerome Korzelius, Roel Janssen, Nicolle Besselink, Sander Boymans, Ruben van Boxtel, Edwin Cuppen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1f6a475491464666a79c0e3f7512c41e
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spelling	oai:doaj.org-article:1f6a475491464666a79c0e3f7512c41e2021-12-02T16:49:45ZThe mutational impact of culturing human pluripotent and adult stem cells10.1038/s41467-020-16323-42041-1723https://doaj.org/article/1f6a475491464666a79c0e3f7512c41e2020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16323-4https://doaj.org/toc/2041-1723Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lowers mutation rates.Ewart KuijkMyrthe JagerBastiaan van der RoestMauro D. LocatiArne Van HoeckJerome KorzeliusRoel JanssenNicolle BesselinkSander BoymansRuben van BoxtelEdwin CuppenNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ewart Kuijk Myrthe Jager Bastiaan van der Roest Mauro D. Locati Arne Van Hoeck Jerome Korzelius Roel Janssen Nicolle Besselink Sander Boymans Ruben van Boxtel Edwin Cuppen The mutational impact of culturing human pluripotent and adult stem cells
description	Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lowers mutation rates.
format	article
author	Ewart Kuijk Myrthe Jager Bastiaan van der Roest Mauro D. Locati Arne Van Hoeck Jerome Korzelius Roel Janssen Nicolle Besselink Sander Boymans Ruben van Boxtel Edwin Cuppen
author_facet	Ewart Kuijk Myrthe Jager Bastiaan van der Roest Mauro D. Locati Arne Van Hoeck Jerome Korzelius Roel Janssen Nicolle Besselink Sander Boymans Ruben van Boxtel Edwin Cuppen
author_sort	Ewart Kuijk
title	The mutational impact of culturing human pluripotent and adult stem cells
title_short	The mutational impact of culturing human pluripotent and adult stem cells
title_full	The mutational impact of culturing human pluripotent and adult stem cells
title_fullStr	The mutational impact of culturing human pluripotent and adult stem cells
title_full_unstemmed	The mutational impact of culturing human pluripotent and adult stem cells
title_sort	mutational impact of culturing human pluripotent and adult stem cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1f6a475491464666a79c0e3f7512c41e
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The mutational impact of culturing human pluripotent and adult stem cells

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