Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases

Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases

Abstract Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesised that the fluid in the space between the SO and the retina, named t...

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Autores principales: Hiroki Kaneko, Kei Takayama, Tetsu Asami, Yasuki Ito, Taichi Tsunekawa, Takeshi Iwase, Yasuhito Funahashi, Shinji Ueno, Norie Nonobe, Shunsuke Yasuda, Ayana Suzumura, Hideyuki Shimizu, Reona Kimoto, Shiang-Jyi Hwang, Hiroko Terasaki
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Science
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Acceso en línea:https://doaj.org/article/1f6b47bb34f34507a91b12c67a632734
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spelling oai:doaj.org-article:1f6b47bb34f34507a91b12c67a6327342021-12-02T16:07:56ZCytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases10.1038/s41598-017-03124-x2045-2322https://doaj.org/article/1f6b47bb34f34507a91b12c67a6327342017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03124-xhttps://doaj.org/toc/2045-2322Abstract Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesised that the fluid in the space between the SO and the retina, named the “sub-silicone oil fluid (SOF)”, enhances these biological responses. We proposed a safe method for SOF extraction. We also analysed inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular hole-associated retinal detachment (MHRD). Interleukin (IL)-10, IL-12p40, IL-6, monocyte chemotactic protein-1, and vascular endothelial growth factor (VEGF) in the SOF with PVR were significantly higher than in those with RRD or MHRD. Fibroblast growth factor-2, IL-10, IL-12p40, IL-8, VEGF, and transforming growth factor beta 1 levels in eyes with exacerbated PDR indicated a significantly higher expression than those with simple PDR. IL-6 and tumour necrosis factor alpha in eyes with exacerbated PVR demonstrated a significantly higher expression than in those with simple PVR. However, there was no difference in SOF osmotic pressure between group of each disease. These studies indicate that disease-specific SOF is a significant reflection of disease status.Hiroki KanekoKei TakayamaTetsu AsamiYasuki ItoTaichi TsunekawaTakeshi IwaseYasuhito FunahashiShinji UenoNorie NonobeShunsuke YasudaAyana SuzumuraHideyuki ShimizuReona KimotoShiang-Jyi HwangHiroko TerasakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hiroki Kaneko
Kei Takayama
Tetsu Asami
Yasuki Ito
Taichi Tsunekawa
Takeshi Iwase
Yasuhito Funahashi
Shinji Ueno
Norie Nonobe
Shunsuke Yasuda
Ayana Suzumura
Hideyuki Shimizu
Reona Kimoto
Shiang-Jyi Hwang
Hiroko Terasaki
Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
description Abstract Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesised that the fluid in the space between the SO and the retina, named the “sub-silicone oil fluid (SOF)”, enhances these biological responses. We proposed a safe method for SOF extraction. We also analysed inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular hole-associated retinal detachment (MHRD). Interleukin (IL)-10, IL-12p40, IL-6, monocyte chemotactic protein-1, and vascular endothelial growth factor (VEGF) in the SOF with PVR were significantly higher than in those with RRD or MHRD. Fibroblast growth factor-2, IL-10, IL-12p40, IL-8, VEGF, and transforming growth factor beta 1 levels in eyes with exacerbated PDR indicated a significantly higher expression than those with simple PDR. IL-6 and tumour necrosis factor alpha in eyes with exacerbated PVR demonstrated a significantly higher expression than in those with simple PVR. However, there was no difference in SOF osmotic pressure between group of each disease. These studies indicate that disease-specific SOF is a significant reflection of disease status.
format article
author Hiroki Kaneko
Kei Takayama
Tetsu Asami
Yasuki Ito
Taichi Tsunekawa
Takeshi Iwase
Yasuhito Funahashi
Shinji Ueno
Norie Nonobe
Shunsuke Yasuda
Ayana Suzumura
Hideyuki Shimizu
Reona Kimoto
Shiang-Jyi Hwang
Hiroko Terasaki
author_facet Hiroki Kaneko
Kei Takayama
Tetsu Asami
Yasuki Ito
Taichi Tsunekawa
Takeshi Iwase
Yasuhito Funahashi
Shinji Ueno
Norie Nonobe
Shunsuke Yasuda
Ayana Suzumura
Hideyuki Shimizu
Reona Kimoto
Shiang-Jyi Hwang
Hiroko Terasaki
author_sort Hiroki Kaneko
title Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
title_short Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
title_full Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
title_fullStr Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
title_full_unstemmed Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
title_sort cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/1f6b47bb34f34507a91b12c67a632734
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