CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.

Loss-of-function mutations in CLMP have been found in patients with Congenital Short Bowel Syndrome (CSBS), suggesting that its encoded protein plays a major role in intestinal development. CLMP is a membrane protein that co-localizes with tight junction proteins, but its function is largely unknown...

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Autores principales:	Christine S van der Werf, Nai-Hua Hsiao, Siobhan Conroy, Joana Paredes, Ana S Ribeiro, Yunia Sribudiani, Raquel Seruca, Robert M W Hofstra, Helga Westers, Sven C D van Ijzendoorn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f77282ca5de420986a7046b9d7ee00a
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spelling	oai:doaj.org-article:1f77282ca5de420986a7046b9d7ee00a2021-11-18T07:55:55ZCLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.1932-620310.1371/journal.pone.0054649https://doaj.org/article/1f77282ca5de420986a7046b9d7ee00a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23460781/?tool=EBIhttps://doaj.org/toc/1932-6203Loss-of-function mutations in CLMP have been found in patients with Congenital Short Bowel Syndrome (CSBS), suggesting that its encoded protein plays a major role in intestinal development. CLMP is a membrane protein that co-localizes with tight junction proteins, but its function is largely unknown. We expressed wild-type (WT)-CLMP and a mutant-CLMP (associated with CSBS) in human intestinal epithelial T84 cells that, as we show here, do not produce endogenous CLMP. We investigated the effects of WT-CLMP and mutant-CLMP proteins on key cellular processes that are important for intestinal epithelial development, including migration, proliferation, viability and transepithelial resistance. Our data showed that expression of WT-CLMP or mutant-CLMP does not affect any of these processes. Moreover, our aggregation assays in CHO cells show that CLMP does not act as a strong adhesion molecule. Thus, our data suggest that, in the in vitro model systems we used, the key processes involved in intestinal epithelial development appear to be unaffected by WT-CLMP or mutant-CLMP. Further research is needed to determine the role of CLMP in the development of the intestine.Christine S van der WerfNai-Hua HsiaoSiobhan ConroyJoana ParedesAna S RibeiroYunia SribudianiRaquel SerucaRobert M W HofstraHelga WestersSven C D van IjzendoornPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e54649 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christine S van der Werf Nai-Hua Hsiao Siobhan Conroy Joana Paredes Ana S Ribeiro Yunia Sribudiani Raquel Seruca Robert M W Hofstra Helga Westers Sven C D van Ijzendoorn CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
description	Loss-of-function mutations in CLMP have been found in patients with Congenital Short Bowel Syndrome (CSBS), suggesting that its encoded protein plays a major role in intestinal development. CLMP is a membrane protein that co-localizes with tight junction proteins, but its function is largely unknown. We expressed wild-type (WT)-CLMP and a mutant-CLMP (associated with CSBS) in human intestinal epithelial T84 cells that, as we show here, do not produce endogenous CLMP. We investigated the effects of WT-CLMP and mutant-CLMP proteins on key cellular processes that are important for intestinal epithelial development, including migration, proliferation, viability and transepithelial resistance. Our data showed that expression of WT-CLMP or mutant-CLMP does not affect any of these processes. Moreover, our aggregation assays in CHO cells show that CLMP does not act as a strong adhesion molecule. Thus, our data suggest that, in the in vitro model systems we used, the key processes involved in intestinal epithelial development appear to be unaffected by WT-CLMP or mutant-CLMP. Further research is needed to determine the role of CLMP in the development of the intestine.
format	article
author	Christine S van der Werf Nai-Hua Hsiao Siobhan Conroy Joana Paredes Ana S Ribeiro Yunia Sribudiani Raquel Seruca Robert M W Hofstra Helga Westers Sven C D van Ijzendoorn
author_facet	Christine S van der Werf Nai-Hua Hsiao Siobhan Conroy Joana Paredes Ana S Ribeiro Yunia Sribudiani Raquel Seruca Robert M W Hofstra Helga Westers Sven C D van Ijzendoorn
author_sort	Christine S van der Werf
title	CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
title_short	CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
title_full	CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
title_fullStr	CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
title_full_unstemmed	CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
title_sort	clmp is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1f77282ca5de420986a7046b9d7ee00a
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CLMP is essential for intestinal development, but does not play a key role in cellular processes involved in intestinal epithelial development.

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