GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii

Abstract Due to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines...

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Autores principales:	Yuka Miki, Kentaro Yoshida, Hiroyuki Enoki, Shoya Komura, Kazuyo Suzuki, Minoru Inamori, Ryo Nishijima, Shigeo Takumi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f7787b59d964102893797845c998e0c
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spelling	oai:doaj.org-article:1f7787b59d964102893797845c998e0c2021-12-02T15:11:50ZGRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii10.1038/s41598-020-78589-42045-2322https://doaj.org/article/1f7787b59d964102893797845c998e0c2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78589-4https://doaj.org/toc/2045-2322Abstract Due to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.Yuka MikiKentaro YoshidaHiroyuki EnokiShoya KomuraKazuyo SuzukiMinoru InamoriRyo NishijimaShigeo TakumiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuka Miki Kentaro Yoshida Hiroyuki Enoki Shoya Komura Kazuyo Suzuki Minoru Inamori Ryo Nishijima Shigeo Takumi GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii
description	Abstract Due to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.
format	article
author	Yuka Miki Kentaro Yoshida Hiroyuki Enoki Shoya Komura Kazuyo Suzuki Minoru Inamori Ryo Nishijima Shigeo Takumi
author_facet	Yuka Miki Kentaro Yoshida Hiroyuki Enoki Shoya Komura Kazuyo Suzuki Minoru Inamori Ryo Nishijima Shigeo Takumi
author_sort	Yuka Miki
title	GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii
title_short	GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii
title_full	GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii
title_fullStr	GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii
title_full_unstemmed	GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii
title_sort	gras-di system facilitates high-density genetic map construction and qtl identification in recombinant inbred lines of the wheat progenitor aegilops tauschii
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1f7787b59d964102893797845c998e0c
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GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii

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