Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples

In this study, we develop a sensitive indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) to detect cadmium (Cd) in cereals. A luminol-hydrogen peroxide chemiluminescence system catalysed by horseradish peroxidase (HRP) was used as the signal detecting system. The method showed a hal...

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Autores principales:	Zhi-Li Xiao, Wei Tian, Zhen-Lin Xu, Yuan-Ming Sun
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	cadmium chemiluminescence immunoassay cereals analysis Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1f7ab01b920d4de3b351256c3b02520f
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spelling	oai:doaj.org-article:1f7ab01b920d4de3b351256c3b02520f2021-11-26T11:19:47ZDevelopment of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples0954-01051465-344310.1080/09540105.2018.1527295https://doaj.org/article/1f7ab01b920d4de3b351256c3b02520f2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1527295https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443In this study, we develop a sensitive indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) to detect cadmium (Cd) in cereals. A luminol-hydrogen peroxide chemiluminescence system catalysed by horseradish peroxidase (HRP) was used as the signal detecting system. The method showed a half-maximal inhibition concentration (IC50) of 0.5 ng/mL and a limit of determination (LOD) of 0.06 ng/mL. The assay indicated an effective linear range from 0.16 to 1.56 ng/mL. There were no obvious cross-reactivities with other metal ions, including Hg2+, Pb2+, As2+, Ag+, Fe3+, Cu2+, Cr2+, Au3+, Ca2+, Mg2+, Mn2+ and Zn2+. Recovery rates ranged from 79.2% to 120.0% for rice, maize, wheat, brown rice and glutinous rice samples. Twenty-five practical cereal samples were analysed, and the comparative analysis of the results showed a strong correlation (r = 0.984) between the CLEIA and the GFAAS methods. The results indicated that the ic-CLEIA developed was acceptable to screen for cadmium in cereal samples.Zhi-Li XiaoWei TianZhen-Lin XuYuan-Ming SunTaylor & Francis Grouparticlecadmiumchemiluminescence immunoassaycerealsanalysisAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1184-1196 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cadmium chemiluminescence immunoassay cereals analysis Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	cadmium chemiluminescence immunoassay cereals analysis Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Zhi-Li Xiao Wei Tian Zhen-Lin Xu Yuan-Ming Sun Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
description	In this study, we develop a sensitive indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) to detect cadmium (Cd) in cereals. A luminol-hydrogen peroxide chemiluminescence system catalysed by horseradish peroxidase (HRP) was used as the signal detecting system. The method showed a half-maximal inhibition concentration (IC50) of 0.5 ng/mL and a limit of determination (LOD) of 0.06 ng/mL. The assay indicated an effective linear range from 0.16 to 1.56 ng/mL. There were no obvious cross-reactivities with other metal ions, including Hg2+, Pb2+, As2+, Ag+, Fe3+, Cu2+, Cr2+, Au3+, Ca2+, Mg2+, Mn2+ and Zn2+. Recovery rates ranged from 79.2% to 120.0% for rice, maize, wheat, brown rice and glutinous rice samples. Twenty-five practical cereal samples were analysed, and the comparative analysis of the results showed a strong correlation (r = 0.984) between the CLEIA and the GFAAS methods. The results indicated that the ic-CLEIA developed was acceptable to screen for cadmium in cereal samples.
format	article
author	Zhi-Li Xiao Wei Tian Zhen-Lin Xu Yuan-Ming Sun
author_facet	Zhi-Li Xiao Wei Tian Zhen-Lin Xu Yuan-Ming Sun
author_sort	Zhi-Li Xiao
title	Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
title_short	Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
title_full	Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
title_fullStr	Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
title_full_unstemmed	Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
title_sort	development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/1f7ab01b920d4de3b351256c3b02520f
work_keys_str_mv	AT zhilixiao developmentofachemiluminescentenzymeimmunoassaytodetectcadmiumincerealsamples AT weitian developmentofachemiluminescentenzymeimmunoassaytodetectcadmiumincerealsamples AT zhenlinxu developmentofachemiluminescentenzymeimmunoassaytodetectcadmiumincerealsamples AT yuanmingsun developmentofachemiluminescentenzymeimmunoassaytodetectcadmiumincerealsamples
_version_	1718409523578798080

Development of a chemiluminescent enzyme immunoassay to detect cadmium in cereal samples

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