Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics

Abstract The tissue metabolomic characteristics associated with endometrial cancer (EC) at different grades were studied using high resolution (400 MHz) magic angle spinning (HR-MAS) proton spectroscopy. The metabolic profiles were obtained from 64 patients (14 with grade 1 (G1), 33 with grade 2 (G2...

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Autores principales:	Agnieszka Skorupa, Michał Poński, Mateusz Ciszek, Bartosz Cichoń, Mateusz Klimek, Andrzej Witek, Sławomir Pakuło, Łukasz Boguszewicz, Maria Sokół
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f7b5a987a844f27b374779f3508b32c
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spelling	oai:doaj.org-article:1f7b5a987a844f27b374779f3508b32c2021-12-02T15:15:40ZGrading of endometrial cancer using 1H HR-MAS NMR-based metabolomics10.1038/s41598-021-97505-y2045-2322https://doaj.org/article/1f7b5a987a844f27b374779f3508b32c2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97505-yhttps://doaj.org/toc/2045-2322Abstract The tissue metabolomic characteristics associated with endometrial cancer (EC) at different grades were studied using high resolution (400 MHz) magic angle spinning (HR-MAS) proton spectroscopy. The metabolic profiles were obtained from 64 patients (14 with grade 1 (G1), 33 with grade 2 (G2) and 17 with grade 3 (G3) tumors) and compared with the profile acquired from 10 patients with the benign disorders. OPLS-DA revealed increased valine, isoleucine, leucine, hypotaurine, serine, lysine, ethanolamine, choline and decreased creatine, creatinine, glutathione, ascorbate, glutamate, phosphoethanolamine and scyllo-inositol in all EC grades in reference to the non-transformed tissue. The increased levels of taurine was additionally detected in the G1 and G2 tumors in comparison to the control tissue, while the elevated glycine, N-acetyl compound and lactate—in the G1 and G3 tumors. The metabolic features typical for the G1 tumors are the increased dimethyl sulfone, phosphocholine, and decreased glycerophosphocholine and glutamine levels, while the decreased myo-inositol level is characteristic for the G2 and G3 tumors. The elevated 3-hydroxybutyrate, alanine and betaine levels were observed in the G3 tumors. The differences between the grade G1 and G3 malignances were mainly related to the perturbations of phosphoethanolamine and phosphocholine biosynthesis, inositol, betaine, serine and glycine metabolism. The statistical significance of the OPLS-DA modeling was also verified by an univariate analysis. HR-MAS NMR based metabolomics provides an useful insight into the metabolic reprogramming in endometrial cancer.Agnieszka SkorupaMichał PońskiMateusz CiszekBartosz CichońMateusz KlimekAndrzej WitekSławomir PakułoŁukasz BoguszewiczMaria SokółNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka Skorupa Michał Poński Mateusz Ciszek Bartosz Cichoń Mateusz Klimek Andrzej Witek Sławomir Pakuło Łukasz Boguszewicz Maria Sokół Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics
description	Abstract The tissue metabolomic characteristics associated with endometrial cancer (EC) at different grades were studied using high resolution (400 MHz) magic angle spinning (HR-MAS) proton spectroscopy. The metabolic profiles were obtained from 64 patients (14 with grade 1 (G1), 33 with grade 2 (G2) and 17 with grade 3 (G3) tumors) and compared with the profile acquired from 10 patients with the benign disorders. OPLS-DA revealed increased valine, isoleucine, leucine, hypotaurine, serine, lysine, ethanolamine, choline and decreased creatine, creatinine, glutathione, ascorbate, glutamate, phosphoethanolamine and scyllo-inositol in all EC grades in reference to the non-transformed tissue. The increased levels of taurine was additionally detected in the G1 and G2 tumors in comparison to the control tissue, while the elevated glycine, N-acetyl compound and lactate—in the G1 and G3 tumors. The metabolic features typical for the G1 tumors are the increased dimethyl sulfone, phosphocholine, and decreased glycerophosphocholine and glutamine levels, while the decreased myo-inositol level is characteristic for the G2 and G3 tumors. The elevated 3-hydroxybutyrate, alanine and betaine levels were observed in the G3 tumors. The differences between the grade G1 and G3 malignances were mainly related to the perturbations of phosphoethanolamine and phosphocholine biosynthesis, inositol, betaine, serine and glycine metabolism. The statistical significance of the OPLS-DA modeling was also verified by an univariate analysis. HR-MAS NMR based metabolomics provides an useful insight into the metabolic reprogramming in endometrial cancer.
format	article
author	Agnieszka Skorupa Michał Poński Mateusz Ciszek Bartosz Cichoń Mateusz Klimek Andrzej Witek Sławomir Pakuło Łukasz Boguszewicz Maria Sokół
author_facet	Agnieszka Skorupa Michał Poński Mateusz Ciszek Bartosz Cichoń Mateusz Klimek Andrzej Witek Sławomir Pakuło Łukasz Boguszewicz Maria Sokół
author_sort	Agnieszka Skorupa
title	Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics
title_short	Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics
title_full	Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics
title_fullStr	Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics
title_full_unstemmed	Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics
title_sort	grading of endometrial cancer using 1h hr-mas nmr-based metabolomics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1f7b5a987a844f27b374779f3508b32c
work_keys_str_mv	AT agnieszkaskorupa gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT michałponski gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT mateuszciszek gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT bartoszcichon gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT mateuszklimek gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT andrzejwitek gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT sławomirpakuło gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT łukaszboguszewicz gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics AT mariasokoł gradingofendometrialcancerusing1hhrmasnmrbasedmetabolomics
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Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics

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