Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.

We structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secon...

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Autores principales:	Julia Gath, Luc Bousset, Birgit Habenstein, Ronald Melki, Anja Böckmann, Beat H Meier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f8e8b5f4b604242897b2a53b763e1d7
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id	oai:doaj.org-article:1f8e8b5f4b604242897b2a53b763e1d7
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spelling	oai:doaj.org-article:1f8e8b5f4b604242897b2a53b763e1d72021-11-18T08:29:37ZUnlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.1932-620310.1371/journal.pone.0090659https://doaj.org/article/1f8e8b5f4b604242897b2a53b763e1d72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24599158/?tool=EBIhttps://doaj.org/toc/1932-6203We structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While β-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified β-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results.Julia GathLuc BoussetBirgit HabensteinRonald MelkiAnja BöckmannBeat H MeierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e90659 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia Gath Luc Bousset Birgit Habenstein Ronald Melki Anja Böckmann Beat H Meier Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
description	We structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While β-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified β-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results.
format	article
author	Julia Gath Luc Bousset Birgit Habenstein Ronald Melki Anja Böckmann Beat H Meier
author_facet	Julia Gath Luc Bousset Birgit Habenstein Ronald Melki Anja Böckmann Beat H Meier
author_sort	Julia Gath
title	Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
title_short	Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
title_full	Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
title_fullStr	Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
title_full_unstemmed	Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
title_sort	unlike twins: an nmr comparison of two α-synuclein polymorphs featuring different toxicity.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1f8e8b5f4b604242897b2a53b763e1d7
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Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.

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