AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets

Abstract Human infections with avian H7N9 subtype influenza viruses are a major public health concern and vaccines against H7N9 are urgently needed for pandemic preparedness. In early 2013, novel H7N9 influenza viruses emerged in China that caused about 1600 human cases of infection with a high asso...

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Autores principales:	Daniel Stadlbauer, Leon de Waal, Edith Beaulieu, Shirin Strohmeier, Edwin J. B. Veldhuis Kroeze, Philippe Boutet, Albert D. M. E. Osterhaus, Florian Krammer, Bruce L. Innis, Raffael Nachbagauer, Koert J. Stittelaar, Corey P. Mallett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/1f961e1904734a6b881c4f9a5ecc6653
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spelling	oai:doaj.org-article:1f961e1904734a6b881c4f9a5ecc66532021-12-02T13:17:48ZAS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets10.1038/s41541-021-00299-32059-0105https://doaj.org/article/1f961e1904734a6b881c4f9a5ecc66532021-03-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00299-3https://doaj.org/toc/2059-0105Abstract Human infections with avian H7N9 subtype influenza viruses are a major public health concern and vaccines against H7N9 are urgently needed for pandemic preparedness. In early 2013, novel H7N9 influenza viruses emerged in China that caused about 1600 human cases of infection with a high associated case fatality rate. In this study, two H7N9 split virion vaccines with or without AS03 adjuvant were tested in the naive ferret model. Serological analyses demonstrated that homologous hemagglutination inhibition and microneutralization antibody titers were detectable in the ferrets after the first immunization with the AS03-adjuvanted vaccines that were further boosted by the second immunization. In addition, heterologous antibody titers against older H7 subtype viruses of the North American lineage (H7N7, H7N3) and newer H7 subtype viruses of the Eurasian lineage (H7N9) were detected in the animals receiving the AS03-adjuvanted vaccines. Animals receiving two immunizations of the AS03-adjuvanted vaccines were protected from weight loss and fever in the homologous challenge study and had no detectable virus in throat or lung samples. In addition, microscopic examination post-challenge showed animals immunized with the AS03-adjuvanted vaccines had the least signs of lung injury and inflammation, consistent with the greater relative efficacy of the adjuvanted vaccines. In conclusion, this study demonstrated that the AS03-adjuvanted H7N9 vaccines elicited high levels of homologous and heterologous antibodies and protected against H7N9 virus damage post-challenge.Daniel StadlbauerLeon de WaalEdith BeaulieuShirin StrohmeierEdwin J. B. Veldhuis KroezePhilippe BoutetAlbert D. M. E. OsterhausFlorian KrammerBruce L. InnisRaffael NachbagauerKoert J. StittelaarCorey P. MallettNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Daniel Stadlbauer Leon de Waal Edith Beaulieu Shirin Strohmeier Edwin J. B. Veldhuis Kroeze Philippe Boutet Albert D. M. E. Osterhaus Florian Krammer Bruce L. Innis Raffael Nachbagauer Koert J. Stittelaar Corey P. Mallett AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
description	Abstract Human infections with avian H7N9 subtype influenza viruses are a major public health concern and vaccines against H7N9 are urgently needed for pandemic preparedness. In early 2013, novel H7N9 influenza viruses emerged in China that caused about 1600 human cases of infection with a high associated case fatality rate. In this study, two H7N9 split virion vaccines with or without AS03 adjuvant were tested in the naive ferret model. Serological analyses demonstrated that homologous hemagglutination inhibition and microneutralization antibody titers were detectable in the ferrets after the first immunization with the AS03-adjuvanted vaccines that were further boosted by the second immunization. In addition, heterologous antibody titers against older H7 subtype viruses of the North American lineage (H7N7, H7N3) and newer H7 subtype viruses of the Eurasian lineage (H7N9) were detected in the animals receiving the AS03-adjuvanted vaccines. Animals receiving two immunizations of the AS03-adjuvanted vaccines were protected from weight loss and fever in the homologous challenge study and had no detectable virus in throat or lung samples. In addition, microscopic examination post-challenge showed animals immunized with the AS03-adjuvanted vaccines had the least signs of lung injury and inflammation, consistent with the greater relative efficacy of the adjuvanted vaccines. In conclusion, this study demonstrated that the AS03-adjuvanted H7N9 vaccines elicited high levels of homologous and heterologous antibodies and protected against H7N9 virus damage post-challenge.
format	article
author	Daniel Stadlbauer Leon de Waal Edith Beaulieu Shirin Strohmeier Edwin J. B. Veldhuis Kroeze Philippe Boutet Albert D. M. E. Osterhaus Florian Krammer Bruce L. Innis Raffael Nachbagauer Koert J. Stittelaar Corey P. Mallett
author_facet	Daniel Stadlbauer Leon de Waal Edith Beaulieu Shirin Strohmeier Edwin J. B. Veldhuis Kroeze Philippe Boutet Albert D. M. E. Osterhaus Florian Krammer Bruce L. Innis Raffael Nachbagauer Koert J. Stittelaar Corey P. Mallett
author_sort	Daniel Stadlbauer
title	AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
title_short	AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
title_full	AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
title_fullStr	AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
title_full_unstemmed	AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
title_sort	as03-adjuvanted h7n9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1f961e1904734a6b881c4f9a5ecc6653
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AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets

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