Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis
Histone deacetylases (HDACs) play crucial roles nearly in all aspects of plant biology, including stress responses, development and growth, and regulation of secondary metabolite biosynthesis. The molecular functions of HDACs have been explored in depth in Arabidopsis thaliana, while little research...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:1f9b1d0d120d4248a3f1f7cabc1ec4b12021-11-17T11:43:56ZIdentification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis1664-462X10.3389/fpls.2021.755494https://doaj.org/article/1f9b1d0d120d4248a3f1f7cabc1ec4b12021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.755494/fullhttps://doaj.org/toc/1664-462XHistone deacetylases (HDACs) play crucial roles nearly in all aspects of plant biology, including stress responses, development and growth, and regulation of secondary metabolite biosynthesis. The molecular functions of HDACs have been explored in depth in Arabidopsis thaliana, while little research has been reported in the medicinal plant Cannabis sativa L. Here, we excavated 14 CsHDAC genes of C. sativa L that were divided into three relatively conserved subfamilies, including RPD3/HDA1 (10 genes), SIR2 (2 genes), and HD2 (2 genes). Genes associated with the biosynthesis of bioactive constituents were identified by combining the distribution of cannabinoids with the expression pattern of HDAC genes in various organs. Using qRT-PCR and transcription group analysis, we verified the expression of candidate genes in different tissues. We found that the histone inhibitor Trichostatin A (TSA) affected the expression of key genes in the cannabinoid metabolism pathway and the accumulation of synthetic precursors, which indirectly indicates that histone inhibitor may regulate the synthesis of active substances in C. sativa L.Liu YangLiu YangXiangxiao MengShilin ChenJun LiWei SunWeiqiang ChenSifan WangHuihua WanGuangtao QianXiaozhe YiJuncan LiYaqin ZhengMing LuoShanshan ChenXia LiuYaolei MiFrontiers Media S.A.articlehistone deacetylaseCannabis sativagene familybioinformaticsgenome-widePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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histone deacetylase Cannabis sativa gene family bioinformatics genome-wide Plant culture SB1-1110 |
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histone deacetylase Cannabis sativa gene family bioinformatics genome-wide Plant culture SB1-1110 Liu Yang Liu Yang Xiangxiao Meng Shilin Chen Jun Li Wei Sun Weiqiang Chen Sifan Wang Huihua Wan Guangtao Qian Xiaozhe Yi Juncan Li Yaqin Zheng Ming Luo Shanshan Chen Xia Liu Yaolei Mi Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis |
| description |
Histone deacetylases (HDACs) play crucial roles nearly in all aspects of plant biology, including stress responses, development and growth, and regulation of secondary metabolite biosynthesis. The molecular functions of HDACs have been explored in depth in Arabidopsis thaliana, while little research has been reported in the medicinal plant Cannabis sativa L. Here, we excavated 14 CsHDAC genes of C. sativa L that were divided into three relatively conserved subfamilies, including RPD3/HDA1 (10 genes), SIR2 (2 genes), and HD2 (2 genes). Genes associated with the biosynthesis of bioactive constituents were identified by combining the distribution of cannabinoids with the expression pattern of HDAC genes in various organs. Using qRT-PCR and transcription group analysis, we verified the expression of candidate genes in different tissues. We found that the histone inhibitor Trichostatin A (TSA) affected the expression of key genes in the cannabinoid metabolism pathway and the accumulation of synthetic precursors, which indirectly indicates that histone inhibitor may regulate the synthesis of active substances in C. sativa L. |
| format |
article |
| author |
Liu Yang Liu Yang Xiangxiao Meng Shilin Chen Jun Li Wei Sun Weiqiang Chen Sifan Wang Huihua Wan Guangtao Qian Xiaozhe Yi Juncan Li Yaqin Zheng Ming Luo Shanshan Chen Xia Liu Yaolei Mi |
| author_facet |
Liu Yang Liu Yang Xiangxiao Meng Shilin Chen Jun Li Wei Sun Weiqiang Chen Sifan Wang Huihua Wan Guangtao Qian Xiaozhe Yi Juncan Li Yaqin Zheng Ming Luo Shanshan Chen Xia Liu Yaolei Mi |
| author_sort |
Liu Yang |
| title |
Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis |
| title_short |
Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis |
| title_full |
Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis |
| title_fullStr |
Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis |
| title_full_unstemmed |
Identification of the Histone Deacetylases Gene Family in Hemp Reveals Genes Regulating Cannabinoids Synthesis |
| title_sort |
identification of the histone deacetylases gene family in hemp reveals genes regulating cannabinoids synthesis |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/1f9b1d0d120d4248a3f1f7cabc1ec4b1 |
| work_keys_str_mv |
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1718425595480637440 |