Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness

Abstract Designer receptor activated by designer drugs (DREADDs) techniques are widely used to modulate the activities of specific neuronal populations during behavioural tasks. However, DREADDs-induced modulation of histaminergic neurons in the tuberomamillary nucleus (HATMN neurons) has produced i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fumito Naganuma, Tadaho Nakamura, Hiroshi Kuroyanagi, Masato Tanaka, Takeo Yoshikawa, Kazuhiko Yanai, Nobuyuki Okamura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f9e12f39843402183a0d3fe3debd20f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1f9e12f39843402183a0d3fe3debd20f
record_format	dspace
spelling	oai:doaj.org-article:1f9e12f39843402183a0d3fe3debd20f2021-12-02T18:03:05ZChemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness10.1038/s41598-021-95497-32045-2322https://doaj.org/article/1f9e12f39843402183a0d3fe3debd20f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95497-3https://doaj.org/toc/2045-2322Abstract Designer receptor activated by designer drugs (DREADDs) techniques are widely used to modulate the activities of specific neuronal populations during behavioural tasks. However, DREADDs-induced modulation of histaminergic neurons in the tuberomamillary nucleus (HATMN neurons) has produced inconsistent effects on the sleep–wake cycle, possibly due to the use of Hdc-Cre mice driving Cre recombinase and DREADDs activity outside the targeted region. Moreover, previous DREADDs studies have not examined locomotor activity and aggressive behaviours, which are also regulated by brain histamine levels. In the present study, we investigated the effects of HATMN activation and inhibition on the locomotor activity, aggressive behaviours and sleep–wake cycle of Hdc-Cre mice with minimal non-target expression of Cre-recombinase. Chemoactivation of HATMN moderately enhanced locomotor activity in a novel open field. Activation of HATMN neurons significantly enhanced aggressive behaviour in the resident–intruder test. Wakefulness was increased and non-rapid eye movement (NREM) sleep decreased for an hour by HATMN chemoactivation. Conversely HATMN chemoinhibition decreased wakefulness and increased NREM sleep for 6 h. These changes in wakefulness induced by HATMN modulation were related to the maintenance of vigilance state. These results indicate the influences of HATMN neurons on exploratory activity, territorial aggression, and wake maintenance.Fumito NaganumaTadaho NakamuraHiroshi KuroyanagiMasato TanakaTakeo YoshikawaKazuhiko YanaiNobuyuki OkamuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fumito Naganuma Tadaho Nakamura Hiroshi Kuroyanagi Masato Tanaka Takeo Yoshikawa Kazuhiko Yanai Nobuyuki Okamura Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
description	Abstract Designer receptor activated by designer drugs (DREADDs) techniques are widely used to modulate the activities of specific neuronal populations during behavioural tasks. However, DREADDs-induced modulation of histaminergic neurons in the tuberomamillary nucleus (HATMN neurons) has produced inconsistent effects on the sleep–wake cycle, possibly due to the use of Hdc-Cre mice driving Cre recombinase and DREADDs activity outside the targeted region. Moreover, previous DREADDs studies have not examined locomotor activity and aggressive behaviours, which are also regulated by brain histamine levels. In the present study, we investigated the effects of HATMN activation and inhibition on the locomotor activity, aggressive behaviours and sleep–wake cycle of Hdc-Cre mice with minimal non-target expression of Cre-recombinase. Chemoactivation of HATMN moderately enhanced locomotor activity in a novel open field. Activation of HATMN neurons significantly enhanced aggressive behaviour in the resident–intruder test. Wakefulness was increased and non-rapid eye movement (NREM) sleep decreased for an hour by HATMN chemoactivation. Conversely HATMN chemoinhibition decreased wakefulness and increased NREM sleep for 6 h. These changes in wakefulness induced by HATMN modulation were related to the maintenance of vigilance state. These results indicate the influences of HATMN neurons on exploratory activity, territorial aggression, and wake maintenance.
format	article
author	Fumito Naganuma Tadaho Nakamura Hiroshi Kuroyanagi Masato Tanaka Takeo Yoshikawa Kazuhiko Yanai Nobuyuki Okamura
author_facet	Fumito Naganuma Tadaho Nakamura Hiroshi Kuroyanagi Masato Tanaka Takeo Yoshikawa Kazuhiko Yanai Nobuyuki Okamura
author_sort	Fumito Naganuma
title	Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
title_short	Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
title_full	Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
title_fullStr	Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
title_full_unstemmed	Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
title_sort	chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1f9e12f39843402183a0d3fe3debd20f
work_keys_str_mv	AT fumitonaganuma chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT tadahonakamura chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT hiroshikuroyanagi chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT masatotanaka chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT takeoyoshikawa chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT kazuhikoyanai chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness AT nobuyukiokamura chemogeneticmodulationofhistaminergicneuronsinthetuberomamillarynucleusaltersterritorialaggressionandwakefulness
_version_	1718378795950407680

Chemogenetic modulation of histaminergic neurons in the tuberomamillary nucleus alters territorial aggression and wakefulness

Ejemplares similares