The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.

Adiponectin is an adipokine secreted by white adipocytes involved in regulating insulin sensitivity in peripheral tissues. Secretion of adiponectin in adipocytes relies on the endosomal system, however, the intracellular machinery involved in mediating adiponectin release is unknown. We have previou...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Brian P Carson, Josep Maria Del Bas, Jose Maria Moreno-Navarrete, Jose Manuel Fernandez-Real, Silvia Mora
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fa1238fa0df44eabc5d0b7d169d23aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1fa1238fa0df44eabc5d0b7d169d23aa
record_format	dspace
spelling	oai:doaj.org-article:1fa1238fa0df44eabc5d0b7d169d23aa2021-11-18T08:55:41ZThe rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.1932-620310.1371/journal.pone.0074687https://doaj.org/article/1fa1238fa0df44eabc5d0b7d169d23aa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24040321/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Adiponectin is an adipokine secreted by white adipocytes involved in regulating insulin sensitivity in peripheral tissues. Secretion of adiponectin in adipocytes relies on the endosomal system, however, the intracellular machinery involved in mediating adiponectin release is unknown. We have previously reported that intracellular adiponectin partially compartmentalizes with rab 5 and rab11, markers for the early/sorting and recycling compartments respectively. Here we have examined the role of several rab11 downstream effector proteins (rab11 FIPs) in regulating adiponectin trafficking and secretion. Overexpression of wild type rab11 FIP1, FIP3 and FIP5 decreased the amount of secreted adiponectin expressed in HEK293 cells, whereas overexpression of rab11 FIP2 or FIP4 had no effect. Furthermore shRNA-mediated depletion of FIP1 enhanced adiponectin release whereas knock down of FIP5 decreased adiponectin secretion. Knock down of FIP3 had no effect. In 3T3L1 adipocytes, endogenous FIP1 co-distributed intracellularly with endogenous adiponectin and FIP1 depletion enhanced adiponectin release without altering insulin-mediated trafficking of the glucose transporter Glut4. While adiponectin receptors internalized with transferrin receptors, there were no differences in transferrin receptor recycling between wild type and FIP1 depleted adipocytes. Consistent with its inhibitory role, FIP1 expression was decreased during adipocyte differentiation, by treatment with thiazolidinediones, and with increased BMI in humans. In contrast, FIP1 expression increased upon exposure of adipocytes to TNFα. In all, our findings identify FIP1 as a novel protein involved in the regulation of adiponectin trafficking and release.Brian P CarsonJosep Maria Del BasJose Maria Moreno-NavarreteJose Manuel Fernandez-RealSilvia MoraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74687 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brian P Carson Josep Maria Del Bas Jose Maria Moreno-Navarrete Jose Manuel Fernandez-Real Silvia Mora The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.
description	Adiponectin is an adipokine secreted by white adipocytes involved in regulating insulin sensitivity in peripheral tissues. Secretion of adiponectin in adipocytes relies on the endosomal system, however, the intracellular machinery involved in mediating adiponectin release is unknown. We have previously reported that intracellular adiponectin partially compartmentalizes with rab 5 and rab11, markers for the early/sorting and recycling compartments respectively. Here we have examined the role of several rab11 downstream effector proteins (rab11 FIPs) in regulating adiponectin trafficking and secretion. Overexpression of wild type rab11 FIP1, FIP3 and FIP5 decreased the amount of secreted adiponectin expressed in HEK293 cells, whereas overexpression of rab11 FIP2 or FIP4 had no effect. Furthermore shRNA-mediated depletion of FIP1 enhanced adiponectin release whereas knock down of FIP5 decreased adiponectin secretion. Knock down of FIP3 had no effect. In 3T3L1 adipocytes, endogenous FIP1 co-distributed intracellularly with endogenous adiponectin and FIP1 depletion enhanced adiponectin release without altering insulin-mediated trafficking of the glucose transporter Glut4. While adiponectin receptors internalized with transferrin receptors, there were no differences in transferrin receptor recycling between wild type and FIP1 depleted adipocytes. Consistent with its inhibitory role, FIP1 expression was decreased during adipocyte differentiation, by treatment with thiazolidinediones, and with increased BMI in humans. In contrast, FIP1 expression increased upon exposure of adipocytes to TNFα. In all, our findings identify FIP1 as a novel protein involved in the regulation of adiponectin trafficking and release.
format	article
author	Brian P Carson Josep Maria Del Bas Jose Maria Moreno-Navarrete Jose Manuel Fernandez-Real Silvia Mora
author_facet	Brian P Carson Josep Maria Del Bas Jose Maria Moreno-Navarrete Jose Manuel Fernandez-Real Silvia Mora
author_sort	Brian P Carson
title	The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.
title_short	The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.
title_full	The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.
title_fullStr	The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.
title_full_unstemmed	The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.
title_sort	rab11 effector protein fip1 regulates adiponectin trafficking and secretion.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1fa1238fa0df44eabc5d0b7d169d23aa
work_keys_str_mv	AT brianpcarson therab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT josepmariadelbas therab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT josemariamorenonavarrete therab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT josemanuelfernandezreal therab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT silviamora therab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT brianpcarson rab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT josepmariadelbas rab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT josemariamorenonavarrete rab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT josemanuelfernandezreal rab11effectorproteinfip1regulatesadiponectintraffickingandsecretion AT silviamora rab11effectorproteinfip1regulatesadiponectintraffickingandsecretion
_version_	1718421129198043136

The rab11 effector protein FIP1 regulates adiponectin trafficking and secretion.

Ejemplares similares