MicroRNA-200b regulates distal airway development by maintaining epithelial integrity
Abstract miR-200b plays a role in epithelial-to-mesenchymal transition (EMT) in cancer. We recently reported abnormal expression of miR-200b in the context of human pulmonary hypoplasia in congenital diaphragmatic hernia (CDH). Smaller lung size, a lower number of airway generations, and a thicker m...
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Nature Portfolio
2017
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oai:doaj.org-article:1fa44ab5d33f4684b3412c079afd0d842021-12-02T16:06:45ZMicroRNA-200b regulates distal airway development by maintaining epithelial integrity10.1038/s41598-017-05412-y2045-2322https://doaj.org/article/1fa44ab5d33f4684b3412c079afd0d842017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05412-yhttps://doaj.org/toc/2045-2322Abstract miR-200b plays a role in epithelial-to-mesenchymal transition (EMT) in cancer. We recently reported abnormal expression of miR-200b in the context of human pulmonary hypoplasia in congenital diaphragmatic hernia (CDH). Smaller lung size, a lower number of airway generations, and a thicker mesenchyme characterize pulmonary hypoplasia in CDH. The aim of this study was to define the role of miR-200b during lung development. Here we show that miR-200b−/− mice have abnormal lung function due to dysfunctional surfactant, increased fibroblast-like cells and thicker mesenchyme in between the alveolar walls. We profiled the lung transcriptome in miR-200b−/− mice, and, using Gene Ontology analysis, we determined that the most affected biological processes include cell cycle, apoptosis and protein transport. Our results demonstrate that miR-200b regulates distal airway development through maintaining an epithelial cell phenotype. The lung abnormalities observed in miR-200b−/− mice recapitulate lung hypoplasia in CDH.Naghmeh KhoshgooRobin VisserLandon FalkChelsea A. DayDustin AmeisBarbara M. IwasiowFuqin ZhuArzu ÖztürkSujata BasuMolly PindAgnes FresnosaMike JacksonVinaya Kumar SiragamGerald StelmackGeoffrey G. HicksAndrew J. HalaykoRichard KeijzerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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DOAJ |
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DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Naghmeh Khoshgoo Robin Visser Landon Falk Chelsea A. Day Dustin Ameis Barbara M. Iwasiow Fuqin Zhu Arzu Öztürk Sujata Basu Molly Pind Agnes Fresnosa Mike Jackson Vinaya Kumar Siragam Gerald Stelmack Geoffrey G. Hicks Andrew J. Halayko Richard Keijzer MicroRNA-200b regulates distal airway development by maintaining epithelial integrity |
| description |
Abstract miR-200b plays a role in epithelial-to-mesenchymal transition (EMT) in cancer. We recently reported abnormal expression of miR-200b in the context of human pulmonary hypoplasia in congenital diaphragmatic hernia (CDH). Smaller lung size, a lower number of airway generations, and a thicker mesenchyme characterize pulmonary hypoplasia in CDH. The aim of this study was to define the role of miR-200b during lung development. Here we show that miR-200b−/− mice have abnormal lung function due to dysfunctional surfactant, increased fibroblast-like cells and thicker mesenchyme in between the alveolar walls. We profiled the lung transcriptome in miR-200b−/− mice, and, using Gene Ontology analysis, we determined that the most affected biological processes include cell cycle, apoptosis and protein transport. Our results demonstrate that miR-200b regulates distal airway development through maintaining an epithelial cell phenotype. The lung abnormalities observed in miR-200b−/− mice recapitulate lung hypoplasia in CDH. |
| format |
article |
| author |
Naghmeh Khoshgoo Robin Visser Landon Falk Chelsea A. Day Dustin Ameis Barbara M. Iwasiow Fuqin Zhu Arzu Öztürk Sujata Basu Molly Pind Agnes Fresnosa Mike Jackson Vinaya Kumar Siragam Gerald Stelmack Geoffrey G. Hicks Andrew J. Halayko Richard Keijzer |
| author_facet |
Naghmeh Khoshgoo Robin Visser Landon Falk Chelsea A. Day Dustin Ameis Barbara M. Iwasiow Fuqin Zhu Arzu Öztürk Sujata Basu Molly Pind Agnes Fresnosa Mike Jackson Vinaya Kumar Siragam Gerald Stelmack Geoffrey G. Hicks Andrew J. Halayko Richard Keijzer |
| author_sort |
Naghmeh Khoshgoo |
| title |
MicroRNA-200b regulates distal airway development by maintaining epithelial integrity |
| title_short |
MicroRNA-200b regulates distal airway development by maintaining epithelial integrity |
| title_full |
MicroRNA-200b regulates distal airway development by maintaining epithelial integrity |
| title_fullStr |
MicroRNA-200b regulates distal airway development by maintaining epithelial integrity |
| title_full_unstemmed |
MicroRNA-200b regulates distal airway development by maintaining epithelial integrity |
| title_sort |
microrna-200b regulates distal airway development by maintaining epithelial integrity |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/1fa44ab5d33f4684b3412c079afd0d84 |
| work_keys_str_mv |
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