Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.

Inflammatory mediators in the tumour microenvironment promote tumour growth, vascular development and enable evasion of anti-tumour immune responses, by disabling infiltrating dendritic cells. However, the constituents of the tumour microenvironment that directly influence dendritic cell maturation...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adriana J Michielsen, Andrew E Hogan, Joseph Marry, Miriam Tosetto, Fionnuala Cox, John M Hyland, Kieran D Sheahan, Diarmuid P O'Donoghue, Hugh E Mulcahy, Elizabeth J Ryan, Jacintha N O'Sullivan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fa55522fd5248f3ba6a2700a7b5a095
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1fa55522fd5248f3ba6a2700a7b5a095
record_format	dspace
spelling	oai:doaj.org-article:1fa55522fd5248f3ba6a2700a7b5a0952021-11-18T07:33:52ZTumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.1932-620310.1371/journal.pone.0027944https://doaj.org/article/1fa55522fd5248f3ba6a2700a7b5a0952011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22125641/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Inflammatory mediators in the tumour microenvironment promote tumour growth, vascular development and enable evasion of anti-tumour immune responses, by disabling infiltrating dendritic cells. However, the constituents of the tumour microenvironment that directly influence dendritic cell maturation and function are not well characterised. Our aim was to identify tumour-associated inflammatory mediators which influence the function of dendritic cells. Tumour conditioned media obtained from cultured colorectal tumour explant tissue contained high levels of the chemokines CCL2, CXCL1, CXCL5 in addition to VEGF. Pre-treatment of monocyte derived dendritic cells with this tumour conditioned media inhibited the up-regulation of CD86, CD83, CD54 and HLA-DR in response to LPS, enhancing IL-10 while reducing IL-12p70 secretion. We examined if specific individual components of the tumour conditioned media (CCL2, CXCL1, CXCL5) could modulate dendritic cell maturation or cytokine secretion in response to LPS. VEGF was also assessed as it has a suppressive effect on dendritic cell maturation. Pre-treatment of immature dendritic cells with VEGF inhibited LPS induced upregulation of CD80 and CD54, while CXCL1 inhibited HLA-DR. Interestingly, treatment of dendritic cells with CCL2, CXCL1, CXCL5 or VEGF significantly suppressed their ability to secrete IL-12p70 in response to LPS. In addition, dendritic cells treated with a combination of CXCL1 and VEGF secreted less IL-12p70 in response to LPS compared to pre-treatment with either cytokine alone. In conclusion, tumour conditioned media strongly influences dendritic cell maturation and function.Adriana J MichielsenAndrew E HoganJoseph MarryMiriam TosettoFionnuala CoxJohn M HylandKieran D SheahanDiarmuid P O'DonoghueHugh E MulcahyElizabeth J RyanJacintha N O'SullivanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27944 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adriana J Michielsen Andrew E Hogan Joseph Marry Miriam Tosetto Fionnuala Cox John M Hyland Kieran D Sheahan Diarmuid P O'Donoghue Hugh E Mulcahy Elizabeth J Ryan Jacintha N O'Sullivan Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
description	Inflammatory mediators in the tumour microenvironment promote tumour growth, vascular development and enable evasion of anti-tumour immune responses, by disabling infiltrating dendritic cells. However, the constituents of the tumour microenvironment that directly influence dendritic cell maturation and function are not well characterised. Our aim was to identify tumour-associated inflammatory mediators which influence the function of dendritic cells. Tumour conditioned media obtained from cultured colorectal tumour explant tissue contained high levels of the chemokines CCL2, CXCL1, CXCL5 in addition to VEGF. Pre-treatment of monocyte derived dendritic cells with this tumour conditioned media inhibited the up-regulation of CD86, CD83, CD54 and HLA-DR in response to LPS, enhancing IL-10 while reducing IL-12p70 secretion. We examined if specific individual components of the tumour conditioned media (CCL2, CXCL1, CXCL5) could modulate dendritic cell maturation or cytokine secretion in response to LPS. VEGF was also assessed as it has a suppressive effect on dendritic cell maturation. Pre-treatment of immature dendritic cells with VEGF inhibited LPS induced upregulation of CD80 and CD54, while CXCL1 inhibited HLA-DR. Interestingly, treatment of dendritic cells with CCL2, CXCL1, CXCL5 or VEGF significantly suppressed their ability to secrete IL-12p70 in response to LPS. In addition, dendritic cells treated with a combination of CXCL1 and VEGF secreted less IL-12p70 in response to LPS compared to pre-treatment with either cytokine alone. In conclusion, tumour conditioned media strongly influences dendritic cell maturation and function.
format	article
author	Adriana J Michielsen Andrew E Hogan Joseph Marry Miriam Tosetto Fionnuala Cox John M Hyland Kieran D Sheahan Diarmuid P O'Donoghue Hugh E Mulcahy Elizabeth J Ryan Jacintha N O'Sullivan
author_facet	Adriana J Michielsen Andrew E Hogan Joseph Marry Miriam Tosetto Fionnuala Cox John M Hyland Kieran D Sheahan Diarmuid P O'Donoghue Hugh E Mulcahy Elizabeth J Ryan Jacintha N O'Sullivan
author_sort	Adriana J Michielsen
title	Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
title_short	Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
title_full	Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
title_fullStr	Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
title_full_unstemmed	Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
title_sort	tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1fa55522fd5248f3ba6a2700a7b5a095
work_keys_str_mv	AT adrianajmichielsen tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT andrewehogan tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT josephmarry tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT miriamtosetto tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT fionnualacox tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT johnmhyland tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT kierandsheahan tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT diarmuidpodonoghue tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT hughemulcahy tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT elizabethjryan tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer AT jacinthanosullivan tumourtissuemicroenvironmentcaninhibitdendriticcellmaturationincolorectalcancer
_version_	1718423272377286656

Tumour tissue microenvironment can inhibit dendritic cell maturation in colorectal cancer.

Ejemplares similares