MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS

The aim of the study was the development of a cell biosensor system based on the fact that AR activator stimuli lead to redistribution of AR from cytoplasm to the nucleus. These changes could be measured and used for the diagnostics and personalized treatment of prostate cancer patient. The stably t...

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Autores principales:	E. P. Khvostova, V. O. Pustylnyak, A. I. Korbut, A. A. Otpuschennikov, L. F. Gulyaeva, I. N. Krylova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2012
Materias:	prostate cancer biosensor system androgen receptor cell lines Science Q
Acceso en línea:	https://doaj.org/article/1fad71cb0a0a404ab861079f293a64ab
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id	oai:doaj.org-article:1fad71cb0a0a404ab861079f293a64ab
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spelling	oai:doaj.org-article:1fad71cb0a0a404ab861079f293a64ab2021-11-23T06:14:19ZMODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS2541-94202587-9596https://doaj.org/article/1fad71cb0a0a404ab861079f293a64ab2012-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/862https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The aim of the study was the development of a cell biosensor system based on the fact that AR activator stimuli lead to redistribution of AR from cytoplasm to the nucleus. These changes could be measured and used for the diagnostics and personalized treatment of prostate cancer patient. The stably transfected cell lines with fluorescently tagged AR were generated. For the stable cell expression the non AR expressing HeLa cell line was used. The constructs of chimeric fluorescent proteins wild type AR or mutant ART877A were used for the transfection. The changes of the CFP-AR-YFP and CFP-ART877A-YFP proteins in the nucleus of HeLa cells under different concentrations steroids treatment was investigated. Moreover, the quantity of activated receptor in the nucleus was carried.E. P. KhvostovaV. O. PustylnyakA. I. KorbutA. A. OtpuschennikovL. F. GulyaevaI. N. KrylovaScientific Сentre for Family Health and Human Reproduction Problemsarticleprostate cancerbiosensor systemandrogen receptorcell linesScienceQRUActa Biomedica Scientifica, Vol 0, Iss 3(1), Pp 83-88 (2012)
institution	DOAJ
collection	DOAJ
language	RU
topic	prostate cancer biosensor system androgen receptor cell lines Science Q
spellingShingle	prostate cancer biosensor system androgen receptor cell lines Science Q E. P. Khvostova V. O. Pustylnyak A. I. Korbut A. A. Otpuschennikov L. F. Gulyaeva I. N. Krylova MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS
description	The aim of the study was the development of a cell biosensor system based on the fact that AR activator stimuli lead to redistribution of AR from cytoplasm to the nucleus. These changes could be measured and used for the diagnostics and personalized treatment of prostate cancer patient. The stably transfected cell lines with fluorescently tagged AR were generated. For the stable cell expression the non AR expressing HeLa cell line was used. The constructs of chimeric fluorescent proteins wild type AR or mutant ART877A were used for the transfection. The changes of the CFP-AR-YFP and CFP-ART877A-YFP proteins in the nucleus of HeLa cells under different concentrations steroids treatment was investigated. Moreover, the quantity of activated receptor in the nucleus was carried.
format	article
author	E. P. Khvostova V. O. Pustylnyak A. I. Korbut A. A. Otpuschennikov L. F. Gulyaeva I. N. Krylova
author_facet	E. P. Khvostova V. O. Pustylnyak A. I. Korbut A. A. Otpuschennikov L. F. Gulyaeva I. N. Krylova
author_sort	E. P. Khvostova
title	MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS
title_short	MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS
title_full	MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS
title_fullStr	MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS
title_full_unstemmed	MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS
title_sort	modern approach to molecular characterization of prostate tumors
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2012
url	https://doaj.org/article/1fad71cb0a0a404ab861079f293a64ab
work_keys_str_mv	AT epkhvostova modernapproachtomolecularcharacterizationofprostatetumors AT vopustylnyak modernapproachtomolecularcharacterizationofprostatetumors AT aikorbut modernapproachtomolecularcharacterizationofprostatetumors AT aaotpuschennikov modernapproachtomolecularcharacterizationofprostatetumors AT lfgulyaeva modernapproachtomolecularcharacterizationofprostatetumors AT inkrylova modernapproachtomolecularcharacterizationofprostatetumors
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MODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORS

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