Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells

Healthy pulp tissue plays an important role in normal function and long-term retention of teeth. Mesoporous bioactive glass (MBG) as a kind of regenerative biomaterials shows the potential in preserving the vital pulp. In this study, MBG prepared by organic template method combined with sol-gel meth...

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Autores principales:	Lin Zhu, Jingyi Li, Yanmei Dong
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Mesoporous bioactive glass Pulp cells Dental pulp dentin complex Medicine R
Acceso en línea:	https://doaj.org/article/1fb06fc9067147d4a36e9fa79ce1bd5c
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spelling	oai:doaj.org-article:1fb06fc9067147d4a36e9fa79ce1bd5c2021-11-25T15:05:12ZEffect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells10.7717/peerj.124212167-8359https://doaj.org/article/1fb06fc9067147d4a36e9fa79ce1bd5c2021-11-01T00:00:00Zhttps://peerj.com/articles/12421.pdfhttps://peerj.com/articles/12421/https://doaj.org/toc/2167-8359Healthy pulp tissue plays an important role in normal function and long-term retention of teeth. Mesoporous bioactive glass (MBG) as a kind of regenerative biomaterials shows the potential in preserving the vital pulp. In this study, MBG prepared by organic template method combined with sol-gel method were used in human dental pulp cell culture and ectopic mineralization experiment. Real-Time PCR was used to explore its ability to induce odontogenic differentiation of dental pulp cells. MBG and rat crowns were implanted under the skin of nude mice for 4 weeks to observe the formation of pulp dentin complex. We found that MBG can release Si and Ca ions and has a strong mineralization activity in vitro. The co-culture of MBG with human dental pulp cells promoted the expression of DMP-1 (dentin matrix protein-1) and ALP (alkalinephosphatase) without affecting cell proliferation. After 4 weeks of subcutaneous implantation in nude mice, the formation of hard tissue with regular structure under the material could be seen, and the structure was similar to dentin tubules. These results indicate that MBG can induce the differentiation of dental pulp cells and the formation of dental pulp-dentin complex and has the potential to promote the repair and regeneration of dental pulp injuries.Lin ZhuJingyi LiYanmei DongPeerJ Inc.articleMesoporous bioactive glassPulp cellsDental pulp dentin complexMedicineRENPeerJ, Vol 9, p e12421 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mesoporous bioactive glass Pulp cells Dental pulp dentin complex Medicine R
spellingShingle	Mesoporous bioactive glass Pulp cells Dental pulp dentin complex Medicine R Lin Zhu Jingyi Li Yanmei Dong Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
description	Healthy pulp tissue plays an important role in normal function and long-term retention of teeth. Mesoporous bioactive glass (MBG) as a kind of regenerative biomaterials shows the potential in preserving the vital pulp. In this study, MBG prepared by organic template method combined with sol-gel method were used in human dental pulp cell culture and ectopic mineralization experiment. Real-Time PCR was used to explore its ability to induce odontogenic differentiation of dental pulp cells. MBG and rat crowns were implanted under the skin of nude mice for 4 weeks to observe the formation of pulp dentin complex. We found that MBG can release Si and Ca ions and has a strong mineralization activity in vitro. The co-culture of MBG with human dental pulp cells promoted the expression of DMP-1 (dentin matrix protein-1) and ALP (alkalinephosphatase) without affecting cell proliferation. After 4 weeks of subcutaneous implantation in nude mice, the formation of hard tissue with regular structure under the material could be seen, and the structure was similar to dentin tubules. These results indicate that MBG can induce the differentiation of dental pulp cells and the formation of dental pulp-dentin complex and has the potential to promote the repair and regeneration of dental pulp injuries.
format	article
author	Lin Zhu Jingyi Li Yanmei Dong
author_facet	Lin Zhu Jingyi Li Yanmei Dong
author_sort	Lin Zhu
title	Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
title_short	Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
title_full	Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
title_fullStr	Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
title_full_unstemmed	Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
title_sort	effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/1fb06fc9067147d4a36e9fa79ce1bd5c
work_keys_str_mv	AT linzhu effectofmesoporousbioactiveglassonodontogenicdifferentiationofhumandentalpulpstemcells AT jingyili effectofmesoporousbioactiveglassonodontogenicdifferentiationofhumandentalpulpstemcells AT yanmeidong effectofmesoporousbioactiveglassonodontogenicdifferentiationofhumandentalpulpstemcells
_version_	1718413366723084288

Effect of mesoporous bioactive glass on odontogenic differentiation of human dental pulp stem cells

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