A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation

Abstract Biocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful i...

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Autores principales:	Jennifer Zehner, Anja Røyne, Pawel Sikorski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fb0cebd79814ba7b1eea9daa43e8a5d
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spelling	oai:doaj.org-article:1fb0cebd79814ba7b1eea9daa43e8a5d2021-12-02T16:10:37ZA sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation10.1038/s41598-021-92235-72045-2322https://doaj.org/article/1fb0cebd79814ba7b1eea9daa43e8a5d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92235-7https://doaj.org/toc/2045-2322Abstract Biocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful implementation of biocementation in large-scale applications requires detailed knowledge about the micro-scale processes of $$\text {CaCO}_{3}$$ CaCO 3 precipitation and grain consolidation. For this purpose, we present a microscopy sample cell that enables real time and in situ observations of the precipitation of $$\text {CaCO}_{3}$$ CaCO 3 in the presence of sand grains and calcite seeds. In this study, the sample cell is used in combination with confocal laser scanning microscopy (CLSM) which allows the monitoring in situ of local pH during the reaction. The sample cell can be disassembled at the end of the experiment, so that the precipitated crystals can be characterized with Raman microspectroscopy and scanning electron microscopy (SEM) without disturbing the sample. The combination of the real time and in situ monitoring of the precipitation process with the possibility to characterize the precipitated crystals without further sample processing, offers a powerful tool for knowledge-based improvements of biocementation.Jennifer ZehnerAnja RøynePawel SikorskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer Zehner Anja Røyne Pawel Sikorski A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
description	Abstract Biocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful implementation of biocementation in large-scale applications requires detailed knowledge about the micro-scale processes of $$\text {CaCO}_{3}$$ CaCO 3 precipitation and grain consolidation. For this purpose, we present a microscopy sample cell that enables real time and in situ observations of the precipitation of $$\text {CaCO}_{3}$$ CaCO 3 in the presence of sand grains and calcite seeds. In this study, the sample cell is used in combination with confocal laser scanning microscopy (CLSM) which allows the monitoring in situ of local pH during the reaction. The sample cell can be disassembled at the end of the experiment, so that the precipitated crystals can be characterized with Raman microspectroscopy and scanning electron microscopy (SEM) without disturbing the sample. The combination of the real time and in situ monitoring of the precipitation process with the possibility to characterize the precipitated crystals without further sample processing, offers a powerful tool for knowledge-based improvements of biocementation.
format	article
author	Jennifer Zehner Anja Røyne Pawel Sikorski
author_facet	Jennifer Zehner Anja Røyne Pawel Sikorski
author_sort	Jennifer Zehner
title	A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
title_short	A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
title_full	A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
title_fullStr	A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
title_full_unstemmed	A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
title_sort	sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1fb0cebd79814ba7b1eea9daa43e8a5d
work_keys_str_mv	AT jenniferzehner asamplecellforthestudyofenzymeinducedcarbonateprecipitationatthegrainscaleanditsimplicationsforbiocementation AT anjarøyne asamplecellforthestudyofenzymeinducedcarbonateprecipitationatthegrainscaleanditsimplicationsforbiocementation AT pawelsikorski asamplecellforthestudyofenzymeinducedcarbonateprecipitationatthegrainscaleanditsimplicationsforbiocementation AT jenniferzehner samplecellforthestudyofenzymeinducedcarbonateprecipitationatthegrainscaleanditsimplicationsforbiocementation AT anjarøyne samplecellforthestudyofenzymeinducedcarbonateprecipitationatthegrainscaleanditsimplicationsforbiocementation AT pawelsikorski samplecellforthestudyofenzymeinducedcarbonateprecipitationatthegrainscaleanditsimplicationsforbiocementation
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A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation

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