Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli

Background The pET expression system based on T7 promoter which is induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) is by far the most commonly used system for production of heterogeneous proteins in Escherichia coli. However, this system was limited by obvious drawbacks including the host to...

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Autores principales:	Fei Shang, Hui Wang, Dan Zhang, Wenhui Wang, Jiangliu Yu, Ting Xue
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	AI-2 quorum sensing Protein expression Auto-induction Escherichia coli Medicine R
Acceso en línea:	https://doaj.org/article/1fb8bb8c024c49cca0ba54782949ab21
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spelling	oai:doaj.org-article:1fb8bb8c024c49cca0ba54782949ab212021-11-18T15:05:39ZConstruction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli10.7717/peerj.124972167-8359https://doaj.org/article/1fb8bb8c024c49cca0ba54782949ab212021-11-01T00:00:00Zhttps://peerj.com/articles/12497.pdfhttps://peerj.com/articles/12497/https://doaj.org/toc/2167-8359Background The pET expression system based on T7 promoter which is induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) is by far the most commonly used system for production of heterogeneous proteins in Escherichia coli. However, this system was limited by obvious drawbacks including the host toxicity and metabolic burden imposed by the presence of IPTG. Methods In this study, we incorporated the autoinducer-2 (AI-2) quorum sensing system to realize autoinduction of the pET expression system. The autoinduction expression vector pXWZ1 was constructed by inserting the lsr promoter regions into the pET28a(+) vector. The expression efficiency of the reporter genes gfpuv and lacZ by the pXWZ1 and pET28a(+) vectors were compared. Results The results showed that the expression levels of the both report genes in the cells transformed with pXWZ1 without any addition of exogenous inducer were higher than that transformed with pET28a(+) vectors by the induction of IPTG. Conclusion This new auto-induction system will exclude the limitations of the IPTG induction including toxic to host and increasing formation of inclusion body and will become a more economical and convenient tool for recombinant protein expression.Fei ShangHui WangDan ZhangWenhui WangJiangliu YuTing XuePeerJ Inc.articleAI-2 quorum sensingProtein expressionAuto-inductionEscherichia coliMedicineRENPeerJ, Vol 9, p e12497 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	AI-2 quorum sensing Protein expression Auto-induction Escherichia coli Medicine R
spellingShingle	AI-2 quorum sensing Protein expression Auto-induction Escherichia coli Medicine R Fei Shang Hui Wang Dan Zhang Wenhui Wang Jiangliu Yu Ting Xue Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli
description	Background The pET expression system based on T7 promoter which is induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) is by far the most commonly used system for production of heterogeneous proteins in Escherichia coli. However, this system was limited by obvious drawbacks including the host toxicity and metabolic burden imposed by the presence of IPTG. Methods In this study, we incorporated the autoinducer-2 (AI-2) quorum sensing system to realize autoinduction of the pET expression system. The autoinduction expression vector pXWZ1 was constructed by inserting the lsr promoter regions into the pET28a(+) vector. The expression efficiency of the reporter genes gfpuv and lacZ by the pXWZ1 and pET28a(+) vectors were compared. Results The results showed that the expression levels of the both report genes in the cells transformed with pXWZ1 without any addition of exogenous inducer were higher than that transformed with pET28a(+) vectors by the induction of IPTG. Conclusion This new auto-induction system will exclude the limitations of the IPTG induction including toxic to host and increasing formation of inclusion body and will become a more economical and convenient tool for recombinant protein expression.
format	article
author	Fei Shang Hui Wang Dan Zhang Wenhui Wang Jiangliu Yu Ting Xue
author_facet	Fei Shang Hui Wang Dan Zhang Wenhui Wang Jiangliu Yu Ting Xue
author_sort	Fei Shang
title	Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli
title_short	Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli
title_full	Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli
title_fullStr	Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli
title_full_unstemmed	Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli
title_sort	construction of an ai-2 quorum sensing induced heterologous protein expression system in escherichia coli
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/1fb8bb8c024c49cca0ba54782949ab21
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Construction of an AI-2 quorum sensing induced heterologous protein expression system in Escherichia coli

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