Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa

Introduction. The present study assessed Pseudomonas aeruginosa surface characteristics, motility and adhesion properties under the influence of 1-[4-(1,1,3,3-tetra methyl butyl) phenoxy]-3-(N-benzyl hexa methylene iminium)-2-propanol chloride (KVM-194). Material and methods. The clinical strain P....

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Autores principales:	Nataliia HRYNCHUK, Tetiana BUKHTIAROVA, Daria DUDIKOVA, Nina VRYNCHANU, Vira NEDASHKIVSKA, Iryna BOIKO, Yurii KOROTKYI, Larisa BONDARENKO
Formato:	article
Lenguaje:	EN FR RO RU
Publicado:	Asociatia de Biosiguranta si Biosecuritate 2021
Materias:	pseudomonas aeruginosa hydrophobicity motility adhesion biofilms Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fb94644ae764c88bdc5b088a7bb3e28
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spelling	oai:doaj.org-article:1fb94644ae764c88bdc5b088a7bb3e282021-12-02T14:37:41ZAnti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa10.38045/ohrm.2021.1.052887-34582587-3466https://doaj.org/article/1fb94644ae764c88bdc5b088a7bb3e282021-01-01T00:00:00Zhttps://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/19/55https://doaj.org/toc/2887-3458https://doaj.org/toc/2587-3466Introduction. The present study assessed Pseudomonas aeruginosa surface characteristics, motility and adhesion properties under the influence of 1-[4-(1,1,3,3-tetra methyl butyl) phenoxy]-3-(N-benzyl hexa methylene iminium)-2-propanol chloride (KVM-194). Material and methods. The clinical strain P. aeruginosa 449 was used in the study. The cell surface hydrophobicity (CSH) was evaluated by adhesion to solvent (MATS test). Swimming, swarming and twitching motility of P. aeruginosa were studied by standard methods in media with different agar contents. Cells ability to adhere to polystyrene was assessed by the Christensen method. The effect of KVM-194, meropenem and ciprofloxacin on hydrophobicity and motility was evaluated both at 0.5 or 2.0 minimal inhibitory concentrations (MIC), while on adhesion abilities – only 0.5×MIC. Results. It was shown that 0.5× MIC KVM-194 reduced CSH of P. aeruginosa (by 16%, p˂0.05), affected swimming motility, and decreased its adhesion to polystyrene. The most pronounced changes in adhesion properties were recorded after 3-5 hours of pre-treatment with this compound. Moreover, it was proven that sub-MICs of meropenem and ciprofloxacin did not alter bacterial cells hydrophobicity and had no significant influence on P. aeruginosa motility and adhesion properties. Conclusions. The present study suggested that KVM-194 affected the initial steps of P. aeruginosa biofilm formation and thus had tremendous potential for new antibiofilm agents’ development.Nataliia HRYNCHUKTetiana BUKHTIAROVADaria DUDIKOVANina VRYNCHANUVira NEDASHKIVSKAIryna BOIKOYurii KOROTKYILarisa BONDARENKOAsociatia de Biosiguranta si Biosecuritatearticlepseudomonas aeruginosahydrophobicitymotilityadhesionbiofilmsMedicineRScienceQENFRRORUOne Health & Risk Management, Vol 2, Iss 1, Pp 34-41 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR RO RU
topic	pseudomonas aeruginosa hydrophobicity motility adhesion biofilms Medicine R Science Q
spellingShingle	pseudomonas aeruginosa hydrophobicity motility adhesion biofilms Medicine R Science Q Nataliia HRYNCHUK Tetiana BUKHTIAROVA Daria DUDIKOVA Nina VRYNCHANU Vira NEDASHKIVSKA Iryna BOIKO Yurii KOROTKYI Larisa BONDARENKO Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa
description	Introduction. The present study assessed Pseudomonas aeruginosa surface characteristics, motility and adhesion properties under the influence of 1-[4-(1,1,3,3-tetra methyl butyl) phenoxy]-3-(N-benzyl hexa methylene iminium)-2-propanol chloride (KVM-194). Material and methods. The clinical strain P. aeruginosa 449 was used in the study. The cell surface hydrophobicity (CSH) was evaluated by adhesion to solvent (MATS test). Swimming, swarming and twitching motility of P. aeruginosa were studied by standard methods in media with different agar contents. Cells ability to adhere to polystyrene was assessed by the Christensen method. The effect of KVM-194, meropenem and ciprofloxacin on hydrophobicity and motility was evaluated both at 0.5 or 2.0 minimal inhibitory concentrations (MIC), while on adhesion abilities – only 0.5×MIC. Results. It was shown that 0.5× MIC KVM-194 reduced CSH of P. aeruginosa (by 16%, p˂0.05), affected swimming motility, and decreased its adhesion to polystyrene. The most pronounced changes in adhesion properties were recorded after 3-5 hours of pre-treatment with this compound. Moreover, it was proven that sub-MICs of meropenem and ciprofloxacin did not alter bacterial cells hydrophobicity and had no significant influence on P. aeruginosa motility and adhesion properties. Conclusions. The present study suggested that KVM-194 affected the initial steps of P. aeruginosa biofilm formation and thus had tremendous potential for new antibiofilm agents’ development.
format	article
author	Nataliia HRYNCHUK Tetiana BUKHTIAROVA Daria DUDIKOVA Nina VRYNCHANU Vira NEDASHKIVSKA Iryna BOIKO Yurii KOROTKYI Larisa BONDARENKO
author_facet	Nataliia HRYNCHUK Tetiana BUKHTIAROVA Daria DUDIKOVA Nina VRYNCHANU Vira NEDASHKIVSKA Iryna BOIKO Yurii KOROTKYI Larisa BONDARENKO
author_sort	Nataliia HRYNCHUK
title	Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa
title_short	Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa
title_full	Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa
title_fullStr	Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa
title_full_unstemmed	Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa
title_sort	anti-adhesion properties of aminopropanol derivative with n-alkylaryl radical kvm-194 against pseudomonas aeruginosa
publisher	Asociatia de Biosiguranta si Biosecuritate
publishDate	2021
url	https://doaj.org/article/1fb94644ae764c88bdc5b088a7bb3e28
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Anti-adhesion properties of aminopropanol derivative with N-alkylaryl radical KVM-194 against Pseudomonas aeruginosa

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