Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations

Abstract Pulsatilla vernalis is a IUCN listed species that occurs in mountain and lowland habitats. The seeds collected from different populations are remarkably diverse in their viability depending on locality or year of collection. We aim to analyse seed viability, among others, by investigation o...

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Autores principales:	Katarzyna M. Zielińska, Andrzej Kaźmierczak, Ewa Michalska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fba5db15288441dbc10f79c1d770f45
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spelling	oai:doaj.org-article:1fba5db15288441dbc10f79c1d770f452021-12-02T16:45:41ZShort-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations10.1038/s41598-021-95668-22045-2322https://doaj.org/article/1fba5db15288441dbc10f79c1d770f452021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95668-2https://doaj.org/toc/2045-2322Abstract Pulsatilla vernalis is a IUCN listed species that occurs in mountain and lowland habitats. The seeds collected from different populations are remarkably diverse in their viability depending on locality or year of collection. We aim to analyse seed viability, among others, by investigation of the percentage of alive, dying, and dead cells in embryos and endosperm when comparing the seeds from a wild lowland population and ex situ cultivation of plants of lowland and Alpine origin. The cell death was detected by staining with two fluorescence probes, one penetrating only the changed nuclear membranes, the other penetrating also the unchanged cells. 54.5% of Alpine origin seeds were presumably capable of germination if they were sown after collection, however, four months later only 36.4% had healthy embryos. In the case of lowland wild plants it was 31.8% and 18.2%, and from ex situ, 27.3% and 13.6%, respectively. 27.3% of Alpine origin seeds had embryo in torpedo stage (9.1% in the case of lowland seeds). Mean weight of the former was 2.9 mg (2.0 mg in lowland ones). Our results confirm the significance of seed origin and seed weight on viability, and that Pulsatilla seeds have a short ‘germination time window’.Katarzyna M. ZielińskaAndrzej KaźmierczakEwa MichalskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna M. Zielińska Andrzej Kaźmierczak Ewa Michalska Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations
description	Abstract Pulsatilla vernalis is a IUCN listed species that occurs in mountain and lowland habitats. The seeds collected from different populations are remarkably diverse in their viability depending on locality or year of collection. We aim to analyse seed viability, among others, by investigation of the percentage of alive, dying, and dead cells in embryos and endosperm when comparing the seeds from a wild lowland population and ex situ cultivation of plants of lowland and Alpine origin. The cell death was detected by staining with two fluorescence probes, one penetrating only the changed nuclear membranes, the other penetrating also the unchanged cells. 54.5% of Alpine origin seeds were presumably capable of germination if they were sown after collection, however, four months later only 36.4% had healthy embryos. In the case of lowland wild plants it was 31.8% and 18.2%, and from ex situ, 27.3% and 13.6%, respectively. 27.3% of Alpine origin seeds had embryo in torpedo stage (9.1% in the case of lowland seeds). Mean weight of the former was 2.9 mg (2.0 mg in lowland ones). Our results confirm the significance of seed origin and seed weight on viability, and that Pulsatilla seeds have a short ‘germination time window’.
format	article
author	Katarzyna M. Zielińska Andrzej Kaźmierczak Ewa Michalska
author_facet	Katarzyna M. Zielińska Andrzej Kaźmierczak Ewa Michalska
author_sort	Katarzyna M. Zielińska
title	Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations
title_short	Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations
title_full	Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations
title_fullStr	Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations
title_full_unstemmed	Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations
title_sort	short-term cell death in tissues of pulsatilla vernalis seeds from natural and ex situ conserved populations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1fba5db15288441dbc10f79c1d770f45
work_keys_str_mv	AT katarzynamzielinska shorttermcelldeathintissuesofpulsatillavernalisseedsfromnaturalandexsituconservedpopulations AT andrzejkazmierczak shorttermcelldeathintissuesofpulsatillavernalisseedsfromnaturalandexsituconservedpopulations AT ewamichalska shorttermcelldeathintissuesofpulsatillavernalisseedsfromnaturalandexsituconservedpopulations
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Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations

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