Quantitative modeling of regular retinal microglia distribution

Abstract Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, and distribution. However, the mechanism underlying the regular distribut...

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Autores principales:	Yoshie Endo, Daisuke Asanuma, Shigeyuki Namiki, Kei Sugihara, Kenzo Hirose, Akiyoshi Uemura, Yoshiaki Kubota, Takashi Miura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fbaeb4317e04215b2358b9a286690c4
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spelling	oai:doaj.org-article:1fbaeb4317e04215b2358b9a286690c42021-11-28T12:16:01ZQuantitative modeling of regular retinal microglia distribution10.1038/s41598-021-01820-32045-2322https://doaj.org/article/1fbaeb4317e04215b2358b9a286690c42021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01820-3https://doaj.org/toc/2045-2322Abstract Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, and distribution. However, the mechanism underlying the regular distribution of microglia remains to be elucidated. First, we quantitatively confirmed the regularity of the distribution pattern of microglial soma in the retina. Second, we formulated a mathematical model that includes factors that may influence regular distribution. Next, we experimentally quantified the model parameters (cell movement, process formation, and ATP dynamics). The resulting model simulation from the measured parameters showed that direct cell–cell contact is most important in generating regular cell spacing. Finally, we tried to specify the molecular pathway responsible for the repulsion between neighboring microglia.Yoshie EndoDaisuke AsanumaShigeyuki NamikiKei SugiharaKenzo HiroseAkiyoshi UemuraYoshiaki KubotaTakashi MiuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshie Endo Daisuke Asanuma Shigeyuki Namiki Kei Sugihara Kenzo Hirose Akiyoshi Uemura Yoshiaki Kubota Takashi Miura Quantitative modeling of regular retinal microglia distribution
description	Abstract Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, and distribution. However, the mechanism underlying the regular distribution of microglia remains to be elucidated. First, we quantitatively confirmed the regularity of the distribution pattern of microglial soma in the retina. Second, we formulated a mathematical model that includes factors that may influence regular distribution. Next, we experimentally quantified the model parameters (cell movement, process formation, and ATP dynamics). The resulting model simulation from the measured parameters showed that direct cell–cell contact is most important in generating regular cell spacing. Finally, we tried to specify the molecular pathway responsible for the repulsion between neighboring microglia.
format	article
author	Yoshie Endo Daisuke Asanuma Shigeyuki Namiki Kei Sugihara Kenzo Hirose Akiyoshi Uemura Yoshiaki Kubota Takashi Miura
author_facet	Yoshie Endo Daisuke Asanuma Shigeyuki Namiki Kei Sugihara Kenzo Hirose Akiyoshi Uemura Yoshiaki Kubota Takashi Miura
author_sort	Yoshie Endo
title	Quantitative modeling of regular retinal microglia distribution
title_short	Quantitative modeling of regular retinal microglia distribution
title_full	Quantitative modeling of regular retinal microglia distribution
title_fullStr	Quantitative modeling of regular retinal microglia distribution
title_full_unstemmed	Quantitative modeling of regular retinal microglia distribution
title_sort	quantitative modeling of regular retinal microglia distribution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1fbaeb4317e04215b2358b9a286690c4
work_keys_str_mv	AT yoshieendo quantitativemodelingofregularretinalmicrogliadistribution AT daisukeasanuma quantitativemodelingofregularretinalmicrogliadistribution AT shigeyukinamiki quantitativemodelingofregularretinalmicrogliadistribution AT keisugihara quantitativemodelingofregularretinalmicrogliadistribution AT kenzohirose quantitativemodelingofregularretinalmicrogliadistribution AT akiyoshiuemura quantitativemodelingofregularretinalmicrogliadistribution AT yoshiakikubota quantitativemodelingofregularretinalmicrogliadistribution AT takashimiura quantitativemodelingofregularretinalmicrogliadistribution
_version_	1718408063062376448

Quantitative modeling of regular retinal microglia distribution

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