Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency

Abstract Fusarium graminearum is a major fungal pathogen affecting crops of worldwide importance. F. graminearum produces type B trichothecene mycotoxins (TCTB), which are not fully eliminated during food and feed processing. Therefore, the best way to minimize TCTB contamination is to develop preve...

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Autores principales:	Valentin Leannec-Rialland, Alejandro Cabezas-Cruz, Vessela Atanasova, Sylvain Chereau, Nadia Ponts, Miray Tonk, Andreas Vilcinskas, Nathalie Ferrer, James J. Valdés, Florence Richard-Forget
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fbbb8463b4f4ca99e2e6d2c5f653607
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spelling	oai:doaj.org-article:1fbbb8463b4f4ca99e2e6d2c5f6536072021-12-02T15:51:13ZTick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency10.1038/s41598-021-86904-w2045-2322https://doaj.org/article/1fbbb8463b4f4ca99e2e6d2c5f6536072021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86904-whttps://doaj.org/toc/2045-2322Abstract Fusarium graminearum is a major fungal pathogen affecting crops of worldwide importance. F. graminearum produces type B trichothecene mycotoxins (TCTB), which are not fully eliminated during food and feed processing. Therefore, the best way to minimize TCTB contamination is to develop prevention strategies. Herein we show that treatment with the reduced form of the γ-core of the tick defensin DefMT3, referred to as TickCore3 (TC3), decreases F. graminearum growth and abrogates TCTB production. The oxidized form of TC3 loses antifungal activity, but retains anti-mycotoxin activity. Molecular dynamics show that TC3 is recruited by specific membrane phospholipids in F. graminearum and that membrane binding of the oxidized form of TC3 is unstable. Capping each of the three cysteine residues of TC3 with methyl groups reduces its inhibitory efficacy. Substitutions of the positively-charged residues lysine (Lys) 6 or arginine 7 by threonine had the highest and the lesser impact, respectively, on the anti-mycotoxin activity of TC3. We conclude that the binding of linear TC3 to F. graminearum membrane phospholipids is required for the antifungal activity of the reduced peptide. Besides, Lys6 appears essential for the anti-mycotoxin activity of the reduced peptide. Our results provide foundation for developing novel and environment-friendly strategies for controlling F. graminearum.Valentin Leannec-RiallandAlejandro Cabezas-CruzVessela AtanasovaSylvain ChereauNadia PontsMiray TonkAndreas VilcinskasNathalie FerrerJames J. ValdésFlorence Richard-ForgetNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentin Leannec-Rialland Alejandro Cabezas-Cruz Vessela Atanasova Sylvain Chereau Nadia Ponts Miray Tonk Andreas Vilcinskas Nathalie Ferrer James J. Valdés Florence Richard-Forget Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
description	Abstract Fusarium graminearum is a major fungal pathogen affecting crops of worldwide importance. F. graminearum produces type B trichothecene mycotoxins (TCTB), which are not fully eliminated during food and feed processing. Therefore, the best way to minimize TCTB contamination is to develop prevention strategies. Herein we show that treatment with the reduced form of the γ-core of the tick defensin DefMT3, referred to as TickCore3 (TC3), decreases F. graminearum growth and abrogates TCTB production. The oxidized form of TC3 loses antifungal activity, but retains anti-mycotoxin activity. Molecular dynamics show that TC3 is recruited by specific membrane phospholipids in F. graminearum and that membrane binding of the oxidized form of TC3 is unstable. Capping each of the three cysteine residues of TC3 with methyl groups reduces its inhibitory efficacy. Substitutions of the positively-charged residues lysine (Lys) 6 or arginine 7 by threonine had the highest and the lesser impact, respectively, on the anti-mycotoxin activity of TC3. We conclude that the binding of linear TC3 to F. graminearum membrane phospholipids is required for the antifungal activity of the reduced peptide. Besides, Lys6 appears essential for the anti-mycotoxin activity of the reduced peptide. Our results provide foundation for developing novel and environment-friendly strategies for controlling F. graminearum.
format	article
author	Valentin Leannec-Rialland Alejandro Cabezas-Cruz Vessela Atanasova Sylvain Chereau Nadia Ponts Miray Tonk Andreas Vilcinskas Nathalie Ferrer James J. Valdés Florence Richard-Forget
author_facet	Valentin Leannec-Rialland Alejandro Cabezas-Cruz Vessela Atanasova Sylvain Chereau Nadia Ponts Miray Tonk Andreas Vilcinskas Nathalie Ferrer James J. Valdés Florence Richard-Forget
author_sort	Valentin Leannec-Rialland
title	Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
title_short	Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
title_full	Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
title_fullStr	Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
title_full_unstemmed	Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
title_sort	tick defensin γ-core reduces fusarium graminearum growth and abrogates mycotoxins production with high efficiency
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1fbbb8463b4f4ca99e2e6d2c5f653607
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Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency

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