CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration

Abstract Muscle satellite cells are essential for muscle regeneration. However, efficient regeneration does not occur without muscle-resident mesenchymal progenitor cells. We show here that bone marrow-derived mesenchymal stromal cells (Bm-MSCs) also facilitate muscle regeneration in Duchenne muscul...

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Autores principales:	Yasushi Maeda, Yasuhiro Yonemochi, Yuki Nakajyo, Hideaki Hidaka, Tokunori Ikeda, Yukio Ando
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fbe282b5083417da0dc11bce6aaf487
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spelling	oai:doaj.org-article:1fbe282b5083417da0dc11bce6aaf4872021-12-02T11:40:13ZCXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration10.1038/s41598-017-02928-12045-2322https://doaj.org/article/1fbe282b5083417da0dc11bce6aaf4872017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02928-1https://doaj.org/toc/2045-2322Abstract Muscle satellite cells are essential for muscle regeneration. However, efficient regeneration does not occur without muscle-resident mesenchymal progenitor cells. We show here that bone marrow-derived mesenchymal stromal cells (Bm-MSCs) also facilitate muscle regeneration in Duchenne muscular dystrophy (DMD) model mice. Bm-MSCs transplanted into peritoneal cavities of DMD model mice with severe muscle degeneration strongly suppressed dystrophic pathology and improved death-related symptoms, which resulted in dramatic lifespan extension. Isolated single myofibers from Bm-MSC-transplanted mice manifested considerably less myofiber splitting compared with myofibers from non-transplanted mice, which indicated that transplantation significantly ameliorated abnormal regeneration. With regard to the number of satellite cells, several cells remained on myofibers from Bm-MSC-transplanted model mice, but satellite cells rarely occurred on myofibers from non-transplanted mice. Also, CXCL12 was crucial for muscle regeneration. CXCL12 facilitated muscle regeneration and paired box protein–7 (PAX7) expression after cardiotoxin-related muscle injury in vivo. The majority of primary muscle satellite cells sorted by integrin-α7 and CD34 expressed CXCR4, a receptor specific for CXCL12. CXCL12 strongly suppressed p-STAT3 expression in these sorted cells in vitro. CXCL12 may therefore influence muscle regeneration through STAT3 signaling in satellite cells. Targeting these proteins in or on muscle satellite cells may improve many degenerative muscle diseases.Yasushi MaedaYasuhiro YonemochiYuki NakajyoHideaki HidakaTokunori IkedaYukio AndoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yasushi Maeda Yasuhiro Yonemochi Yuki Nakajyo Hideaki Hidaka Tokunori Ikeda Yukio Ando CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
description	Abstract Muscle satellite cells are essential for muscle regeneration. However, efficient regeneration does not occur without muscle-resident mesenchymal progenitor cells. We show here that bone marrow-derived mesenchymal stromal cells (Bm-MSCs) also facilitate muscle regeneration in Duchenne muscular dystrophy (DMD) model mice. Bm-MSCs transplanted into peritoneal cavities of DMD model mice with severe muscle degeneration strongly suppressed dystrophic pathology and improved death-related symptoms, which resulted in dramatic lifespan extension. Isolated single myofibers from Bm-MSC-transplanted mice manifested considerably less myofiber splitting compared with myofibers from non-transplanted mice, which indicated that transplantation significantly ameliorated abnormal regeneration. With regard to the number of satellite cells, several cells remained on myofibers from Bm-MSC-transplanted model mice, but satellite cells rarely occurred on myofibers from non-transplanted mice. Also, CXCL12 was crucial for muscle regeneration. CXCL12 facilitated muscle regeneration and paired box protein–7 (PAX7) expression after cardiotoxin-related muscle injury in vivo. The majority of primary muscle satellite cells sorted by integrin-α7 and CD34 expressed CXCR4, a receptor specific for CXCL12. CXCL12 strongly suppressed p-STAT3 expression in these sorted cells in vitro. CXCL12 may therefore influence muscle regeneration through STAT3 signaling in satellite cells. Targeting these proteins in or on muscle satellite cells may improve many degenerative muscle diseases.
format	article
author	Yasushi Maeda Yasuhiro Yonemochi Yuki Nakajyo Hideaki Hidaka Tokunori Ikeda Yukio Ando
author_facet	Yasushi Maeda Yasuhiro Yonemochi Yuki Nakajyo Hideaki Hidaka Tokunori Ikeda Yukio Ando
author_sort	Yasushi Maeda
title	CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
title_short	CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
title_full	CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
title_fullStr	CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
title_full_unstemmed	CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
title_sort	cxcl12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1fbe282b5083417da0dc11bce6aaf487
work_keys_str_mv	AT yasushimaeda cxcl12andosteopontinfrombonemarrowderivedmesenchymalstromalcellsimprovemuscleregeneration AT yasuhiroyonemochi cxcl12andosteopontinfrombonemarrowderivedmesenchymalstromalcellsimprovemuscleregeneration AT yukinakajyo cxcl12andosteopontinfrombonemarrowderivedmesenchymalstromalcellsimprovemuscleregeneration AT hideakihidaka cxcl12andosteopontinfrombonemarrowderivedmesenchymalstromalcellsimprovemuscleregeneration AT tokunoriikeda cxcl12andosteopontinfrombonemarrowderivedmesenchymalstromalcellsimprovemuscleregeneration AT yukioando cxcl12andosteopontinfrombonemarrowderivedmesenchymalstromalcellsimprovemuscleregeneration
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CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration

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