Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia

Abstract A new cell type, interstitial Cajal-like cell (ICLC), was recently described in different organs. The name was recently changed to telocytes (TCs), and their typical thin, long processes have been named telopodes (Tp). TCs regulate the contractile activity of smooth muscle cells and play a...

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Autores principales:	Natalia V. Nizyaeva, Tatiana V. Sukhacheva, Roman A. Serov, Galina V. Kulikova, Marina N. Nagovitsyna, Natalia E. Kan, Victor L. Tyutyunnik, Stanislav V. Pavlovich, Rimma A. Poltavtseva, Ekaterina L. Yarotskaya, Aleksandr I. Shchegolev, Gennadiy T. Sukhikh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fc2e638d38b45699d31d312ebee763a
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spelling	oai:doaj.org-article:1fc2e638d38b45699d31d312ebee763a2021-12-02T16:08:13ZUltrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia10.1038/s41598-018-21492-w2045-2322https://doaj.org/article/1fc2e638d38b45699d31d312ebee763a2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21492-whttps://doaj.org/toc/2045-2322Abstract A new cell type, interstitial Cajal-like cell (ICLC), was recently described in different organs. The name was recently changed to telocytes (TCs), and their typical thin, long processes have been named telopodes (Tp). TCs regulate the contractile activity of smooth muscle cells and play a role in regulating vessel contractions. Although the placenta is not an innervated organ, we believe that TCs are present in the placenta. We studied placenta samples from physiological pregnancies and in different variants of preeclampsia (PE). We examined these samples using light microscopy of semi-thin sections, transmission electron microscopy, and immunohistochemistry. Immunohistochemical examination was performed with primary antibodies to CD34, CD117, SMA, and vimentin, and TMEM16a (DOG-1), the latter was used for the diagnosis of gastrointestinal stromal tumours (GIST) consisting of TCs. We have identified a heterogenetic population of ТСs in term placentas, as these cell types differed in their localization, immunophenotype and ultrastructural characteristics. We assume TMEM16a could be used as the marker for identification of TCs. In PE we have revealed telocyte-like cells with ultrastructural signs of fibrocytes (significant process thickening and the granular endoplasmic reticulum content was increased) and a loss of TMEM16a immunohistochemical staining.Natalia V. NizyaevaTatiana V. SukhachevaRoman A. SerovGalina V. KulikovaMarina N. NagovitsynaNatalia E. KanVictor L. TyutyunnikStanislav V. PavlovichRimma A. PoltavtsevaEkaterina L. YarotskayaAleksandr I. ShchegolevGennadiy T. SukhikhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalia V. Nizyaeva Tatiana V. Sukhacheva Roman A. Serov Galina V. Kulikova Marina N. Nagovitsyna Natalia E. Kan Victor L. Tyutyunnik Stanislav V. Pavlovich Rimma A. Poltavtseva Ekaterina L. Yarotskaya Aleksandr I. Shchegolev Gennadiy T. Sukhikh Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
description	Abstract A new cell type, interstitial Cajal-like cell (ICLC), was recently described in different organs. The name was recently changed to telocytes (TCs), and their typical thin, long processes have been named telopodes (Tp). TCs regulate the contractile activity of smooth muscle cells and play a role in regulating vessel contractions. Although the placenta is not an innervated organ, we believe that TCs are present in the placenta. We studied placenta samples from physiological pregnancies and in different variants of preeclampsia (PE). We examined these samples using light microscopy of semi-thin sections, transmission electron microscopy, and immunohistochemistry. Immunohistochemical examination was performed with primary antibodies to CD34, CD117, SMA, and vimentin, and TMEM16a (DOG-1), the latter was used for the diagnosis of gastrointestinal stromal tumours (GIST) consisting of TCs. We have identified a heterogenetic population of ТСs in term placentas, as these cell types differed in their localization, immunophenotype and ultrastructural characteristics. We assume TMEM16a could be used as the marker for identification of TCs. In PE we have revealed telocyte-like cells with ultrastructural signs of fibrocytes (significant process thickening and the granular endoplasmic reticulum content was increased) and a loss of TMEM16a immunohistochemical staining.
format	article
author	Natalia V. Nizyaeva Tatiana V. Sukhacheva Roman A. Serov Galina V. Kulikova Marina N. Nagovitsyna Natalia E. Kan Victor L. Tyutyunnik Stanislav V. Pavlovich Rimma A. Poltavtseva Ekaterina L. Yarotskaya Aleksandr I. Shchegolev Gennadiy T. Sukhikh
author_facet	Natalia V. Nizyaeva Tatiana V. Sukhacheva Roman A. Serov Galina V. Kulikova Marina N. Nagovitsyna Natalia E. Kan Victor L. Tyutyunnik Stanislav V. Pavlovich Rimma A. Poltavtseva Ekaterina L. Yarotskaya Aleksandr I. Shchegolev Gennadiy T. Sukhikh
author_sort	Natalia V. Nizyaeva
title	Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
title_short	Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
title_full	Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
title_fullStr	Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
title_full_unstemmed	Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
title_sort	ultrastructural and immunohistochemical features of telocytes in placental villi in preeclampsia
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1fc2e638d38b45699d31d312ebee763a
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Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia

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