The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.

In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based infections due to its ability to establish bio...

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Autores principales:	Pilar Gutiérrez-Escribano, Ute Zeidler, M Belén Suárez, Sophie Bachellier-Bassi, Andrés Clemente-Blanco, Julie Bonhomme, Carlos R Vázquez de Aldana, Christophe d'Enfert, Jaime Correa-Bordes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1fc5733caf534b42a9740f6c9058ae0c
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spelling	oai:doaj.org-article:1fc5733caf534b42a9740f6c9058ae0c2021-11-18T06:04:27ZThe NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.1553-73661553-737410.1371/journal.ppat.1002683https://doaj.org/article/1fc5733caf534b42a9740f6c9058ae0c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22589718/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based infections due to its ability to establish biofilms. The transcription factor Bcr1 is a master regulator of C. albicans biofilm development, although the full extent of its regulation remains unknown. Here, we report that Bcr1 is a phosphoprotein that physically interacts with the NDR kinase Cbk1 and undergoes Cbk1-dependent phosphorylation. Mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to alanine markedly impaired Bcr1 function during biofilm formation and virulence in a mouse model of disseminated candidiasis. Cells lacking Cbk1, or any of its upstream activators, also had reduced biofilm development. Notably, mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to glutamate in cbk1Δ cells upregulated the transcription of Bcr1-dependent genes and partially rescued the biofilm defects of a cbk1Δ strain. Therefore, our data uncovered a novel role of the NDR/LATS kinase Cbk1 in the regulation of biofilm development through the control of Bcr1.Pilar Gutiérrez-EscribanoUte ZeidlerM Belén SuárezSophie Bachellier-BassiAndrés Clemente-BlancoJulie BonhommeCarlos R Vázquez de AldanaChristophe d'EnfertJaime Correa-BordesPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 5, p e1002683 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Pilar Gutiérrez-Escribano Ute Zeidler M Belén Suárez Sophie Bachellier-Bassi Andrés Clemente-Blanco Julie Bonhomme Carlos R Vázquez de Aldana Christophe d'Enfert Jaime Correa-Bordes The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.
description	In nature, many microorganisms form specialized complex, multicellular, surface-attached communities called biofilms. These communities play critical roles in microbial pathogenesis. The fungal pathogen Candida albicans is associated with catheter-based infections due to its ability to establish biofilms. The transcription factor Bcr1 is a master regulator of C. albicans biofilm development, although the full extent of its regulation remains unknown. Here, we report that Bcr1 is a phosphoprotein that physically interacts with the NDR kinase Cbk1 and undergoes Cbk1-dependent phosphorylation. Mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to alanine markedly impaired Bcr1 function during biofilm formation and virulence in a mouse model of disseminated candidiasis. Cells lacking Cbk1, or any of its upstream activators, also had reduced biofilm development. Notably, mutating the two putative Cbk1 phosphoacceptor residues in Bcr1 to glutamate in cbk1Δ cells upregulated the transcription of Bcr1-dependent genes and partially rescued the biofilm defects of a cbk1Δ strain. Therefore, our data uncovered a novel role of the NDR/LATS kinase Cbk1 in the regulation of biofilm development through the control of Bcr1.
format	article
author	Pilar Gutiérrez-Escribano Ute Zeidler M Belén Suárez Sophie Bachellier-Bassi Andrés Clemente-Blanco Julie Bonhomme Carlos R Vázquez de Aldana Christophe d'Enfert Jaime Correa-Bordes
author_facet	Pilar Gutiérrez-Escribano Ute Zeidler M Belén Suárez Sophie Bachellier-Bassi Andrés Clemente-Blanco Julie Bonhomme Carlos R Vázquez de Aldana Christophe d'Enfert Jaime Correa-Bordes
author_sort	Pilar Gutiérrez-Escribano
title	The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.
title_short	The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.
title_full	The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.
title_fullStr	The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.
title_full_unstemmed	The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.
title_sort	ndr/lats kinase cbk1 controls the activity of the transcriptional regulator bcr1 during biofilm formation in candida albicans.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1fc5733caf534b42a9740f6c9058ae0c
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The NDR/LATS kinase Cbk1 controls the activity of the transcriptional regulator Bcr1 during biofilm formation in Candida albicans.

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