Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.

<h4>Background and purpose</h4>Longitudinal functional imaging studies of stroke are key in identifying the disease progression and possible therapeutic interventions. Here we investigate the applicability of real-time functional optoacoustic imaging for monitoring of stroke progression...

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Autores principales:	Moritz Kneipp, Jake Turner, Sebastian Hambauer, Sandro M Krieg, Jens Lehmberg, Ute Lindauer, Daniel Razansky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fc6799d2e4a4692ba3ab3e59c0ca46c
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spelling	oai:doaj.org-article:1fc6799d2e4a4692ba3ab3e59c0ca46c2021-11-18T08:21:25ZFunctional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.1932-620310.1371/journal.pone.0096118https://doaj.org/article/1fc6799d2e4a4692ba3ab3e59c0ca46c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24776997/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and purpose</h4>Longitudinal functional imaging studies of stroke are key in identifying the disease progression and possible therapeutic interventions. Here we investigate the applicability of real-time functional optoacoustic imaging for monitoring of stroke progression in the whole brain of living animals.<h4>Materials and methods</h4>The middle cerebral artery occlusion (MCAO) was used to model stroke in mice, which were imaged preoperatively and the occlusion was kept in place for 60 minutes, after which optoacoustic scans were taken at several time points.<h4>Results</h4>Post ischemia an asymmetry of deoxygenated hemoglobin in the brain was observed as a region of hypoxia in the hemisphere affected by the ischemic event. Furthermore, we were able to visualize the penumbra in-vivo as a localized hemodynamically-compromised area adjacent to the region of stroke-induced perfusion deficit.<h4>Conclusion</h4>The intrinsic sensitivity of the new imaging approach to functional blood parameters, in combination with real time operation and high spatial resolution in deep living tissues, may see it become a valuable and unique tool in the development and monitoring of treatments aimed at suspending the spread of an infarct area.Moritz KneippJake TurnerSebastian HambauerSandro M KriegJens LehmbergUte LindauerDaniel RazanskyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e96118 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moritz Kneipp Jake Turner Sebastian Hambauer Sandro M Krieg Jens Lehmberg Ute Lindauer Daniel Razansky Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
description	<h4>Background and purpose</h4>Longitudinal functional imaging studies of stroke are key in identifying the disease progression and possible therapeutic interventions. Here we investigate the applicability of real-time functional optoacoustic imaging for monitoring of stroke progression in the whole brain of living animals.<h4>Materials and methods</h4>The middle cerebral artery occlusion (MCAO) was used to model stroke in mice, which were imaged preoperatively and the occlusion was kept in place for 60 minutes, after which optoacoustic scans were taken at several time points.<h4>Results</h4>Post ischemia an asymmetry of deoxygenated hemoglobin in the brain was observed as a region of hypoxia in the hemisphere affected by the ischemic event. Furthermore, we were able to visualize the penumbra in-vivo as a localized hemodynamically-compromised area adjacent to the region of stroke-induced perfusion deficit.<h4>Conclusion</h4>The intrinsic sensitivity of the new imaging approach to functional blood parameters, in combination with real time operation and high spatial resolution in deep living tissues, may see it become a valuable and unique tool in the development and monitoring of treatments aimed at suspending the spread of an infarct area.
format	article
author	Moritz Kneipp Jake Turner Sebastian Hambauer Sandro M Krieg Jens Lehmberg Ute Lindauer Daniel Razansky
author_facet	Moritz Kneipp Jake Turner Sebastian Hambauer Sandro M Krieg Jens Lehmberg Ute Lindauer Daniel Razansky
author_sort	Moritz Kneipp
title	Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
title_short	Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
title_full	Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
title_fullStr	Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
title_full_unstemmed	Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
title_sort	functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1fc6799d2e4a4692ba3ab3e59c0ca46c
work_keys_str_mv	AT moritzkneipp functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice AT jaketurner functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice AT sebastianhambauer functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice AT sandromkrieg functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice AT jenslehmberg functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice AT utelindauer functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice AT danielrazansky functionalrealtimeoptoacousticimagingofmiddlecerebralarteryocclusioninmice
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Functional real-time optoacoustic imaging of middle cerebral artery occlusion in mice.

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