The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases

Abstract Background: Migration, production of reactive oxygen species (ROS), release of myeloperoxidase (MPO), and NETosis are functional immunological reactions of elementary importance for polymorphonuclear neutrophils (PMN). Unregulated inflammatory response of PMN within tissues plays a key role...

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Autores principales:	Richard F. Kraus, Michael A. Gruber, Martin Kieninger
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	extracellular inflammatory disease matrix neutrophil function tissue Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1fc8a27042a74874a60af568d22828bc
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spelling	oai:doaj.org-article:1fc8a27042a74874a60af568d22828bc2021-11-12T19:57:14ZThe influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases2050-452710.1002/iid3.472https://doaj.org/article/1fc8a27042a74874a60af568d22828bc2021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.472https://doaj.org/toc/2050-4527Abstract Background: Migration, production of reactive oxygen species (ROS), release of myeloperoxidase (MPO), and NETosis are functional immunological reactions of elementary importance for polymorphonuclear neutrophils (PMN). Unregulated inflammatory response of PMN within tissues plays a key role in the pathophysiology of several diseases. However, little is known about the behavior of PMN after migration through blood vessel walls. Therefore, we investigated the influence of the extracellular matrix (ECM) on PMN function. Materials and Methods: We established an in vitro chemotaxis model of type I and III collagen, fibrin, and herbal agarose tissues using µ‐slide chemotaxis devices and N‐formylmethionine‐leucyl‐phenylalanine (fMLP). PMN within the matrices were assessed with a fluorescent time‐lapse microscope for live‐cell imaging. Results: PMN function was obviously influenced by the ECM. Type III collagen had an inhibitory effect on PMN migration regarding track length, direction, and targeting. Type III collagen also had an accelerating effect on neutrophil ROS production. Agarose had an inhibitory effect on MPO release and fibrin a retarding effect on NETosis. Conclusion: Because of the high abundance of type III collagen in lung and skin matrices, the interaction of PMN with the respective matrix could be an important mechanism in the pathophysiology of acute respiratory distress syndrome and pyoderma gangrenosum.Richard F. KrausMichael A. GruberMartin KieningerWileyarticleextracellularinflammatory diseasematrixneutrophil functiontissueImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1237-1251 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular inflammatory disease matrix neutrophil function tissue Immunologic diseases. Allergy RC581-607
spellingShingle	extracellular inflammatory disease matrix neutrophil function tissue Immunologic diseases. Allergy RC581-607 Richard F. Kraus Michael A. Gruber Martin Kieninger The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
description	Abstract Background: Migration, production of reactive oxygen species (ROS), release of myeloperoxidase (MPO), and NETosis are functional immunological reactions of elementary importance for polymorphonuclear neutrophils (PMN). Unregulated inflammatory response of PMN within tissues plays a key role in the pathophysiology of several diseases. However, little is known about the behavior of PMN after migration through blood vessel walls. Therefore, we investigated the influence of the extracellular matrix (ECM) on PMN function. Materials and Methods: We established an in vitro chemotaxis model of type I and III collagen, fibrin, and herbal agarose tissues using µ‐slide chemotaxis devices and N‐formylmethionine‐leucyl‐phenylalanine (fMLP). PMN within the matrices were assessed with a fluorescent time‐lapse microscope for live‐cell imaging. Results: PMN function was obviously influenced by the ECM. Type III collagen had an inhibitory effect on PMN migration regarding track length, direction, and targeting. Type III collagen also had an accelerating effect on neutrophil ROS production. Agarose had an inhibitory effect on MPO release and fibrin a retarding effect on NETosis. Conclusion: Because of the high abundance of type III collagen in lung and skin matrices, the interaction of PMN with the respective matrix could be an important mechanism in the pathophysiology of acute respiratory distress syndrome and pyoderma gangrenosum.
format	article
author	Richard F. Kraus Michael A. Gruber Martin Kieninger
author_facet	Richard F. Kraus Michael A. Gruber Martin Kieninger
author_sort	Richard F. Kraus
title	The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
title_short	The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
title_full	The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
title_fullStr	The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
title_full_unstemmed	The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
title_sort	influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/1fc8a27042a74874a60af568d22828bc
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The influence of extracellular tissue on neutrophil function and its possible linkage to inflammatory diseases

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