Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China

Abstract Background Human astrovirus (HAstV) and sapovirus (SaV) are common pathogens that can cause acute gastroenteritis (AGE). However, very few studies have reported the molecular epidemiology and clinical information on HAstV and SaV in China. This study aims to determine the molecular epidemio...

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Autores principales:	Xin Luo, Jian-kai Deng, Xiao-ping Mu, Nan Yu, Xiaoyan Che
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Acute gastroenteritis Astrovirus Sapovirus Clinical features Molecular epidemiology Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/1fce7607442f40c4a8f06ffa11b9a5cc
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spelling	oai:doaj.org-article:1fce7607442f40c4a8f06ffa11b9a5cc2021-12-05T12:11:00ZDetection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China10.1186/s12876-021-02044-51471-230Xhttps://doaj.org/article/1fce7607442f40c4a8f06ffa11b9a5cc2021-12-01T00:00:00Zhttps://doi.org/10.1186/s12876-021-02044-5https://doaj.org/toc/1471-230XAbstract Background Human astrovirus (HAstV) and sapovirus (SaV) are common pathogens that can cause acute gastroenteritis (AGE). However, very few studies have reported the molecular epidemiology and clinical information on HAstV and SaV in China. This study aims to determine the molecular epidemiology and clinical features of HAstV and SaV in patients with AGE in Guangzhou, China. Methods For this study, 656 patients with AGE were enrolled. Their stool samples were screened for 15 enteropathogens using Luminex xTAG® Gastrointestinal Pathogen Panel. HAstV and SaV were detected through an in-house multiplex reverse transcriptase polymerase chain reaction followed by phylogenetic analysis. We described and compared clinical features of AGE in patients with HAstV and SaV. Results Of the 656 stool samples, 63.72% (418/656) were found to be positive, with 550 enteropathogens (296 bacteria and 254 viruses). HAstV and SaV were detected in 20 (3.0%) and 12 (1.8%) samples, respectively. Four genotypes (genotypes 1, 2, 3, and 8) of HAstV and three genotypes (GI.1, GI.2 and GIV) of SaV were identified. Coinfection was observed in ten HAstV-positive and two SaV-positive samples. HAstV was more likely to occur in winter, while SaV in early spring. The median age of the patients with single HAstV infection was higher than that of the patients with other viruses (rotavirus, norovirus, and enteric adenovirus; P = 0.0476) and unknown etiology (P = 0.006). Coinfection with HAstV or SaV were not associated with disease severity (P > 0.05). Conclusion HAstV and SaV are the common causes of AGE in Guangzhou, China.Xin LuoJian-kai DengXiao-ping MuNan YuXiaoyan CheBMCarticleAcute gastroenteritisAstrovirusSapovirusClinical featuresMolecular epidemiologyDiseases of the digestive system. GastroenterologyRC799-869ENBMC Gastroenterology, Vol 21, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acute gastroenteritis Astrovirus Sapovirus Clinical features Molecular epidemiology Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	Acute gastroenteritis Astrovirus Sapovirus Clinical features Molecular epidemiology Diseases of the digestive system. Gastroenterology RC799-869 Xin Luo Jian-kai Deng Xiao-ping Mu Nan Yu Xiaoyan Che Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
description	Abstract Background Human astrovirus (HAstV) and sapovirus (SaV) are common pathogens that can cause acute gastroenteritis (AGE). However, very few studies have reported the molecular epidemiology and clinical information on HAstV and SaV in China. This study aims to determine the molecular epidemiology and clinical features of HAstV and SaV in patients with AGE in Guangzhou, China. Methods For this study, 656 patients with AGE were enrolled. Their stool samples were screened for 15 enteropathogens using Luminex xTAG® Gastrointestinal Pathogen Panel. HAstV and SaV were detected through an in-house multiplex reverse transcriptase polymerase chain reaction followed by phylogenetic analysis. We described and compared clinical features of AGE in patients with HAstV and SaV. Results Of the 656 stool samples, 63.72% (418/656) were found to be positive, with 550 enteropathogens (296 bacteria and 254 viruses). HAstV and SaV were detected in 20 (3.0%) and 12 (1.8%) samples, respectively. Four genotypes (genotypes 1, 2, 3, and 8) of HAstV and three genotypes (GI.1, GI.2 and GIV) of SaV were identified. Coinfection was observed in ten HAstV-positive and two SaV-positive samples. HAstV was more likely to occur in winter, while SaV in early spring. The median age of the patients with single HAstV infection was higher than that of the patients with other viruses (rotavirus, norovirus, and enteric adenovirus; P = 0.0476) and unknown etiology (P = 0.006). Coinfection with HAstV or SaV were not associated with disease severity (P > 0.05). Conclusion HAstV and SaV are the common causes of AGE in Guangzhou, China.
format	article
author	Xin Luo Jian-kai Deng Xiao-ping Mu Nan Yu Xiaoyan Che
author_facet	Xin Luo Jian-kai Deng Xiao-ping Mu Nan Yu Xiaoyan Che
author_sort	Xin Luo
title	Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
title_short	Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
title_full	Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
title_fullStr	Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
title_full_unstemmed	Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
title_sort	detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in guangzhou, china
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1fce7607442f40c4a8f06ffa11b9a5cc
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Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China

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