Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells

Abstract Recent work demonstrated that 3D fibrin scaffolds function as an effective substrate for engineering tissues from pluripotent stem cells. However, the rapid degradation rate of fibrin remains a major limitation when differentiating human pluripotent stem cells for tissue engineering applica...

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Autores principales:	Meghan Robinson, Sarah Douglas, Stephanie Michelle Willerth
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fd021cd6c554581991f4bfa2dc887de
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spelling	oai:doaj.org-article:1fd021cd6c554581991f4bfa2dc887de2021-12-02T11:52:35ZMechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells10.1038/s41598-017-06570-92045-2322https://doaj.org/article/1fd021cd6c554581991f4bfa2dc887de2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06570-9https://doaj.org/toc/2045-2322Abstract Recent work demonstrated that 3D fibrin scaffolds function as an effective substrate for engineering tissues from pluripotent stem cells. However, the rapid degradation rate of fibrin remains a major limitation when differentiating human pluripotent stem cells for tissue engineering applications. The addition of crosslinking agents, such as genipin, during the polymerization process increases scaffold stability while decreasing the degradation rate of fibrin. Genipin crosslinking alters the physical characteristics of the fibrin scaffolds, which influences the behaviour of the differentiating cells seeded inside. It also possesses neuritogenic and neuroprotective properties, making it particularly attractive for engineering neural tissue from pluripotent stem cells. Here we show that genipin enhances neuronal differentiation of neural progenitors derived from human induced pluripotent stem cells (hiPSCs) in 2D culture and genipin concentration influences the morphological and mechanical properties of 3D fibrin scaffolds. These mechanically stable genipin-crosslinked fibrin scaffolds support hiPSC-derived neural aggregates and induce neurite outgrowth while remaining intact for 2 weeks as opposed to 5 days for unmodified fibrin scaffolds.Meghan RobinsonSarah DouglasStephanie Michelle WillerthNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meghan Robinson Sarah Douglas Stephanie Michelle Willerth Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
description	Abstract Recent work demonstrated that 3D fibrin scaffolds function as an effective substrate for engineering tissues from pluripotent stem cells. However, the rapid degradation rate of fibrin remains a major limitation when differentiating human pluripotent stem cells for tissue engineering applications. The addition of crosslinking agents, such as genipin, during the polymerization process increases scaffold stability while decreasing the degradation rate of fibrin. Genipin crosslinking alters the physical characteristics of the fibrin scaffolds, which influences the behaviour of the differentiating cells seeded inside. It also possesses neuritogenic and neuroprotective properties, making it particularly attractive for engineering neural tissue from pluripotent stem cells. Here we show that genipin enhances neuronal differentiation of neural progenitors derived from human induced pluripotent stem cells (hiPSCs) in 2D culture and genipin concentration influences the morphological and mechanical properties of 3D fibrin scaffolds. These mechanically stable genipin-crosslinked fibrin scaffolds support hiPSC-derived neural aggregates and induce neurite outgrowth while remaining intact for 2 weeks as opposed to 5 days for unmodified fibrin scaffolds.
format	article
author	Meghan Robinson Sarah Douglas Stephanie Michelle Willerth
author_facet	Meghan Robinson Sarah Douglas Stephanie Michelle Willerth
author_sort	Meghan Robinson
title	Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
title_short	Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
title_full	Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
title_fullStr	Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
title_full_unstemmed	Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
title_sort	mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1fd021cd6c554581991f4bfa2dc887de
work_keys_str_mv	AT meghanrobinson mechanicallystablefibrinscaffoldspromoteviabilityandinduceneuriteoutgrowthinneuralaggregatesderivedfromhumaninducedpluripotentstemcells AT sarahdouglas mechanicallystablefibrinscaffoldspromoteviabilityandinduceneuriteoutgrowthinneuralaggregatesderivedfromhumaninducedpluripotentstemcells AT stephaniemichellewillerth mechanicallystablefibrinscaffoldspromoteviabilityandinduceneuriteoutgrowthinneuralaggregatesderivedfromhumaninducedpluripotentstemcells
_version_	1718394970792001536

Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells

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