CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis

Microsatellite genotyping is a common molecular method of for identification but current methods have restricted throughput. The authors demonstrate STR-Seq, which combines next generation sequencing with targetedin vitroCRISPR-Cas9 fragmentation to enable massively parallel analysis in the thousand...

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Autores principales:	GiWon Shin, Susan M. Grimes, HoJoon Lee, Billy T. Lau, Li C. Xia, Hanlee P. Ji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1fd514e535d042ffa2a18a3e8a9b6a4c
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spelling	oai:doaj.org-article:1fd514e535d042ffa2a18a3e8a9b6a4c2021-12-02T14:41:18ZCRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis10.1038/ncomms142912041-1723https://doaj.org/article/1fd514e535d042ffa2a18a3e8a9b6a4c2017-02-01T00:00:00Zhttps://doi.org/10.1038/ncomms14291https://doaj.org/toc/2041-1723Microsatellite genotyping is a common molecular method of for identification but current methods have restricted throughput. The authors demonstrate STR-Seq, which combines next generation sequencing with targetedin vitroCRISPR-Cas9 fragmentation to enable massively parallel analysis in the thousands.GiWon ShinSusan M. GrimesHoJoon LeeBilly T. LauLi C. XiaHanlee P. JiNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q GiWon Shin Susan M. Grimes HoJoon Lee Billy T. Lau Li C. Xia Hanlee P. Ji CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
description	Microsatellite genotyping is a common molecular method of for identification but current methods have restricted throughput. The authors demonstrate STR-Seq, which combines next generation sequencing with targetedin vitroCRISPR-Cas9 fragmentation to enable massively parallel analysis in the thousands.
format	article
author	GiWon Shin Susan M. Grimes HoJoon Lee Billy T. Lau Li C. Xia Hanlee P. Ji
author_facet	GiWon Shin Susan M. Grimes HoJoon Lee Billy T. Lau Li C. Xia Hanlee P. Ji
author_sort	GiWon Shin
title	CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
title_short	CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
title_full	CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
title_fullStr	CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
title_full_unstemmed	CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
title_sort	crispr–cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1fd514e535d042ffa2a18a3e8a9b6a4c
work_keys_str_mv	AT giwonshin crisprcas9targetedfragmentationandselectivesequencingenablemassivelyparallelmicrosatelliteanalysis AT susanmgrimes crisprcas9targetedfragmentationandselectivesequencingenablemassivelyparallelmicrosatelliteanalysis AT hojoonlee crisprcas9targetedfragmentationandselectivesequencingenablemassivelyparallelmicrosatelliteanalysis AT billytlau crisprcas9targetedfragmentationandselectivesequencingenablemassivelyparallelmicrosatelliteanalysis AT licxia crisprcas9targetedfragmentationandselectivesequencingenablemassivelyparallelmicrosatelliteanalysis AT hanleepji crisprcas9targetedfragmentationandselectivesequencingenablemassivelyparallelmicrosatelliteanalysis
_version_	1718389916864348160

CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis

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