Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR

SARS-CoV-2 infection is widely diagnosed by RT-PCR, but RNA extraction is a bottleneck for fast and cheap diagnosis. Here, the authors develop protocols to perform RT-PCR directly on heat-inactivated subject samples or samples lysed with readily available detergents and benchmark performance against...

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Autores principales:	Ioanna Smyrlaki, Martin Ekman, Antonio Lentini, Nuno Rufino de Sousa, Natali Papanicolaou, Martin Vondracek, Johan Aarum, Hamzah Safari, Shaman Muradrasoli, Antonio Gigliotti Rothfuchs, Jan Albert, Björn Högberg, Björn Reinius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1fdca452b94243fe9b1c96011a64ebaf
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spelling	oai:doaj.org-article:1fdca452b94243fe9b1c96011a64ebaf2021-12-02T18:14:31ZMassive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR10.1038/s41467-020-18611-52041-1723https://doaj.org/article/1fdca452b94243fe9b1c96011a64ebaf2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18611-5https://doaj.org/toc/2041-1723SARS-CoV-2 infection is widely diagnosed by RT-PCR, but RNA extraction is a bottleneck for fast and cheap diagnosis. Here, the authors develop protocols to perform RT-PCR directly on heat-inactivated subject samples or samples lysed with readily available detergents and benchmark performance against 597 clinically diagnosed patient samples.Ioanna SmyrlakiMartin EkmanAntonio LentiniNuno Rufino de SousaNatali PapanicolaouMartin VondracekJohan AarumHamzah SafariShaman MuradrasoliAntonio Gigliotti RothfuchsJan AlbertBjörn HögbergBjörn ReiniusNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ioanna Smyrlaki Martin Ekman Antonio Lentini Nuno Rufino de Sousa Natali Papanicolaou Martin Vondracek Johan Aarum Hamzah Safari Shaman Muradrasoli Antonio Gigliotti Rothfuchs Jan Albert Björn Högberg Björn Reinius Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
description	SARS-CoV-2 infection is widely diagnosed by RT-PCR, but RNA extraction is a bottleneck for fast and cheap diagnosis. Here, the authors develop protocols to perform RT-PCR directly on heat-inactivated subject samples or samples lysed with readily available detergents and benchmark performance against 597 clinically diagnosed patient samples.
format	article
author	Ioanna Smyrlaki Martin Ekman Antonio Lentini Nuno Rufino de Sousa Natali Papanicolaou Martin Vondracek Johan Aarum Hamzah Safari Shaman Muradrasoli Antonio Gigliotti Rothfuchs Jan Albert Björn Högberg Björn Reinius
author_facet	Ioanna Smyrlaki Martin Ekman Antonio Lentini Nuno Rufino de Sousa Natali Papanicolaou Martin Vondracek Johan Aarum Hamzah Safari Shaman Muradrasoli Antonio Gigliotti Rothfuchs Jan Albert Björn Högberg Björn Reinius
author_sort	Ioanna Smyrlaki
title	Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
title_short	Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
title_full	Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
title_fullStr	Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
title_full_unstemmed	Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
title_sort	massive and rapid covid-19 testing is feasible by extraction-free sars-cov-2 rt-pcr
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1fdca452b94243fe9b1c96011a64ebaf
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Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR

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