Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species

ABSTRACT Systemic infections of Candida species pose a significant threat to public health. Toxicity associated with current therapies and emergence of resistant strains present major therapeutic challenges. Here, we report exploitation of the probiotic properties of two novel, food-derived yeasts,...

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Autores principales:	Lohith Kunyeit, Nawneet K. Kurrey, K. A. Anu-Appaiah, Reeta P. Rao
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	probiotic yeasts plastic adhesion assay Caco-2 cell monolayer mixed-species Candida biofilm Candida tropicalis Candida krusei Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1fdd3bb98eae475489da5f7a747a334f
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spelling	oai:doaj.org-article:1fdd3bb98eae475489da5f7a747a334f2021-11-15T15:59:41ZProbiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species10.1128/mBio.02307-192150-7511https://doaj.org/article/1fdd3bb98eae475489da5f7a747a334f2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02307-19https://doaj.org/toc/2150-7511ABSTRACT Systemic infections of Candida species pose a significant threat to public health. Toxicity associated with current therapies and emergence of resistant strains present major therapeutic challenges. Here, we report exploitation of the probiotic properties of two novel, food-derived yeasts, Saccharomyces cerevisiae (strain KTP) and Issatchenkia occidentalis (strain ApC), as an alternative approach to combat widespread opportunistic fungal infections. Both yeasts inhibit virulence traits such as adhesion, filamentation, and biofilm formation of several non-albicans Candida species, including Candida tropicalis, Candida krusei, Candida glabrata, and Candida parapsilosis as well as the recently identified multidrug-resistant species Candida auris. They inhibit adhesion to abiotic surfaces as well as cultured colon epithelial cells. Furthermore, probiotic treatment blocks the formation of biofilms of individual non-albicans Candida strains as well as mixed-culture biofilms of each non-albicans Candida strain in combination with Candida albicans. The probiotic yeasts attenuated non-albicans Candida infections in a live animal. In vivo studies using Caenorhabditis elegans suggest that exposure to probiotic yeasts protects nematodes from infection with non-albicans Candida strains compared to worms that were not exposed to the probiotic yeasts. Furthermore, application of probiotic yeasts postinfection with non-albicans Candida alleviated pathogenic colonization of the nematode gut. The probiotic properties of these novel yeasts are better than or comparable to those of the commercially available probiotic yeast Saccharomyces boulardii, which was used as a reference strain throughout this study. These results indicate that yeasts derived from food sources could serve as an effective alternative to antifungal therapy against emerging pathogenic Candida species. IMPORTANCE Non-albicans Candida-associated infections have emerged as a major risk factor in the hospitalized and immunecompromised patients. Besides, antifungal-associated complications occur more frequently with these non-albicans Candida species than with C. albicans. Therefore, as an alternative approach to combat these widespread non-albicans Candida-associated infections, here we showed the probiotic effect of two yeasts, Saccharomyces cerevisiae (strain KTP) and Issatchenkia occidentalis (ApC), in preventing adhesion and biofilm formation of five non-albicans Candida strains, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis, and Candida auris. The result would influence the current trend of the conversion of conventional antimicrobial therapy into beneficial probiotic microbe-associated antimicrobial treatment.Lohith KunyeitNawneet K. KurreyK. A. Anu-AppaiahReeta P. RaoAmerican Society for Microbiologyarticleprobiotic yeastsplastic adhesion assayCaco-2 cell monolayermixed-species Candida biofilmCandida tropicalisCandida kruseiMicrobiologyQR1-502ENmBio, Vol 10, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotic yeasts plastic adhesion assay Caco-2 cell monolayer mixed-species Candida biofilm Candida tropicalis Candida krusei Microbiology QR1-502
spellingShingle	probiotic yeasts plastic adhesion assay Caco-2 cell monolayer mixed-species Candida biofilm Candida tropicalis Candida krusei Microbiology QR1-502 Lohith Kunyeit Nawneet K. Kurrey K. A. Anu-Appaiah Reeta P. Rao Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species
description	ABSTRACT Systemic infections of Candida species pose a significant threat to public health. Toxicity associated with current therapies and emergence of resistant strains present major therapeutic challenges. Here, we report exploitation of the probiotic properties of two novel, food-derived yeasts, Saccharomyces cerevisiae (strain KTP) and Issatchenkia occidentalis (strain ApC), as an alternative approach to combat widespread opportunistic fungal infections. Both yeasts inhibit virulence traits such as adhesion, filamentation, and biofilm formation of several non-albicans Candida species, including Candida tropicalis, Candida krusei, Candida glabrata, and Candida parapsilosis as well as the recently identified multidrug-resistant species Candida auris. They inhibit adhesion to abiotic surfaces as well as cultured colon epithelial cells. Furthermore, probiotic treatment blocks the formation of biofilms of individual non-albicans Candida strains as well as mixed-culture biofilms of each non-albicans Candida strain in combination with Candida albicans. The probiotic yeasts attenuated non-albicans Candida infections in a live animal. In vivo studies using Caenorhabditis elegans suggest that exposure to probiotic yeasts protects nematodes from infection with non-albicans Candida strains compared to worms that were not exposed to the probiotic yeasts. Furthermore, application of probiotic yeasts postinfection with non-albicans Candida alleviated pathogenic colonization of the nematode gut. The probiotic properties of these novel yeasts are better than or comparable to those of the commercially available probiotic yeast Saccharomyces boulardii, which was used as a reference strain throughout this study. These results indicate that yeasts derived from food sources could serve as an effective alternative to antifungal therapy against emerging pathogenic Candida species. IMPORTANCE Non-albicans Candida-associated infections have emerged as a major risk factor in the hospitalized and immunecompromised patients. Besides, antifungal-associated complications occur more frequently with these non-albicans Candida species than with C. albicans. Therefore, as an alternative approach to combat these widespread non-albicans Candida-associated infections, here we showed the probiotic effect of two yeasts, Saccharomyces cerevisiae (strain KTP) and Issatchenkia occidentalis (ApC), in preventing adhesion and biofilm formation of five non-albicans Candida strains, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis, and Candida auris. The result would influence the current trend of the conversion of conventional antimicrobial therapy into beneficial probiotic microbe-associated antimicrobial treatment.
format	article
author	Lohith Kunyeit Nawneet K. Kurrey K. A. Anu-Appaiah Reeta P. Rao
author_facet	Lohith Kunyeit Nawneet K. Kurrey K. A. Anu-Appaiah Reeta P. Rao
author_sort	Lohith Kunyeit
title	Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species
title_short	Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species
title_full	Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species
title_fullStr	Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species
title_full_unstemmed	Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species
title_sort	probiotic yeasts inhibit virulence of non<italic toggle="yes">-albicans candida</italic> species
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/1fdd3bb98eae475489da5f7a747a334f
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Probiotic Yeasts Inhibit Virulence of Non<italic toggle="yes">-albicans Candida</italic> Species

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