Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
Calmodulin (CaM), as an important factor in the calcium signaling pathway, is widely involved in plant growth and development regulation and responses to external stimuli. In this study, the full-length sequence of the <i>ScCaM</i> gene (GenBank: GQ246454) was isolated from the leaves of...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/1fe40108a87545bf951cf81453b699f2 |
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| Sumario: | Calmodulin (CaM), as an important factor in the calcium signaling pathway, is widely involved in plant growth and development regulation and responses to external stimuli. In this study, the full-length sequence of the <i>ScCaM</i> gene (GenBank: GQ246454) was isolated from the leaves of a <i>Saccharum</i> spp. hybrid. Prokaryotic expression showed that <i>ScCaM</i> could be solubly expressed and purified in <i>Escherichia coli</i> BL21. Subcellular localization confirmed that <i>ScCaM</i> was localized in the plasma membrane and nucleus of cells. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that <i>ScCaM</i> can be induced by various stresses, including sodium chloride (NaCl), chromium trichloride (CrCl<sub>3</sub>), salicylic acid (SA), and methyl jasmonate (MeJA). Ectopic expression in <i>Arabidopsis thaliana</i> demonstrated that <i>ScCaM</i> can affect the growth and development of transgenic plants. Moreover, the qRT-PCR analysis indicated that the overexpression of the allogenic <i>ScCaM</i> gene inhibits the expression of <i>AtSTM</i>, leading to the phenomenon of multiple-tillering in transgenic <i>A. thaliana</i>. The present study provided valuable information and facilitates further investigation into the function of <i>ScCaM</i> in the future. |
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