Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane

Calmodulin (CaM), as an important factor in the calcium signaling pathway, is widely involved in plant growth and development regulation and responses to external stimuli. In this study, the full-length sequence of the <i>ScCaM</i> gene (GenBank: GQ246454) was isolated from the leaves of...

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Autores principales:	Jinxian Liu, Chang Zhang, Weihua Su, Guangheng Wu, Xianyu Fu, Youxiong Que, Jun Luo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sugarcane calmodulin prokaryotic expression subcellular localization stress transient overexpression Agriculture S
Acceso en línea:	https://doaj.org/article/1fe40108a87545bf951cf81453b699f2
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spelling	oai:doaj.org-article:1fe40108a87545bf951cf81453b699f22021-11-25T16:04:33ZIdentification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane10.3390/agronomy111121532073-4395https://doaj.org/article/1fe40108a87545bf951cf81453b699f22021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2153https://doaj.org/toc/2073-4395Calmodulin (CaM), as an important factor in the calcium signaling pathway, is widely involved in plant growth and development regulation and responses to external stimuli. In this study, the full-length sequence of the <i>ScCaM</i> gene (GenBank: GQ246454) was isolated from the leaves of a <i>Saccharum</i> spp. hybrid. Prokaryotic expression showed that <i>ScCaM</i> could be solubly expressed and purified in <i>Escherichia coli</i> BL21. Subcellular localization confirmed that <i>ScCaM</i> was localized in the plasma membrane and nucleus of cells. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that <i>ScCaM</i> can be induced by various stresses, including sodium chloride (NaCl), chromium trichloride (CrCl<sub>3</sub>), salicylic acid (SA), and methyl jasmonate (MeJA). Ectopic expression in <i>Arabidopsis thaliana</i> demonstrated that <i>ScCaM</i> can affect the growth and development of transgenic plants. Moreover, the qRT-PCR analysis indicated that the overexpression of the allogenic <i>ScCaM</i> gene inhibits the expression of <i>AtSTM</i>, leading to the phenomenon of multiple-tillering in transgenic <i>A. thaliana</i>. The present study provided valuable information and facilitates further investigation into the function of <i>ScCaM</i> in the future.Jinxian LiuChang ZhangWeihua SuGuangheng WuXianyu FuYouxiong QueJun LuoMDPI AGarticlesugarcanecalmodulinprokaryotic expressionsubcellular localizationstresstransient overexpressionAgricultureSENAgronomy, Vol 11, Iss 2153, p 2153 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sugarcane calmodulin prokaryotic expression subcellular localization stress transient overexpression Agriculture S
spellingShingle	sugarcane calmodulin prokaryotic expression subcellular localization stress transient overexpression Agriculture S Jinxian Liu Chang Zhang Weihua Su Guangheng Wu Xianyu Fu Youxiong Que Jun Luo Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
description	Calmodulin (CaM), as an important factor in the calcium signaling pathway, is widely involved in plant growth and development regulation and responses to external stimuli. In this study, the full-length sequence of the <i>ScCaM</i> gene (GenBank: GQ246454) was isolated from the leaves of a <i>Saccharum</i> spp. hybrid. Prokaryotic expression showed that <i>ScCaM</i> could be solubly expressed and purified in <i>Escherichia coli</i> BL21. Subcellular localization confirmed that <i>ScCaM</i> was localized in the plasma membrane and nucleus of cells. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that <i>ScCaM</i> can be induced by various stresses, including sodium chloride (NaCl), chromium trichloride (CrCl<sub>3</sub>), salicylic acid (SA), and methyl jasmonate (MeJA). Ectopic expression in <i>Arabidopsis thaliana</i> demonstrated that <i>ScCaM</i> can affect the growth and development of transgenic plants. Moreover, the qRT-PCR analysis indicated that the overexpression of the allogenic <i>ScCaM</i> gene inhibits the expression of <i>AtSTM</i>, leading to the phenomenon of multiple-tillering in transgenic <i>A. thaliana</i>. The present study provided valuable information and facilitates further investigation into the function of <i>ScCaM</i> in the future.
format	article
author	Jinxian Liu Chang Zhang Weihua Su Guangheng Wu Xianyu Fu Youxiong Que Jun Luo
author_facet	Jinxian Liu Chang Zhang Weihua Su Guangheng Wu Xianyu Fu Youxiong Que Jun Luo
author_sort	Jinxian Liu
title	Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
title_short	Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
title_full	Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
title_fullStr	Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
title_full_unstemmed	Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane
title_sort	identification, expression, and functional characterization of <i>sccam</i> in response to various stresses in sugarcane
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1fe40108a87545bf951cf81453b699f2
work_keys_str_mv	AT jinxianliu identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane AT changzhang identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane AT weihuasu identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane AT guanghengwu identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane AT xianyufu identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane AT youxiongque identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane AT junluo identificationexpressionandfunctionalcharacterizationofisccamiinresponsetovariousstressesinsugarcane
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Identification, Expression, and Functional Characterization of <i>ScCaM</i> in Response to Various Stresses in Sugarcane

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