IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China

IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China

ABSTRACT An emerging multidrug-resistant Klebsiella pneumoniae high-risk clone of sequence type 307 (ST307) has been increasingly reported worldwide. Here, we described the genomic characteristics of an IMP-38-producing ST307 K. pneumoniae strain and investigated the prevalence of blaIMP-38 among ca...

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Autores principales: Siyi Wang, Juan Zhao, Ning Liu, Fang Yang, Yiming Zhong, Xiumei Gu, Zijuan Jian, Qun Yan, Qingxia Liu, Hongling Li, Yanming Li, Jing Liu, Hui Li, Liang Chen, Wenen Liu
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2020
Materias:
carbapenem-resistant Klebsiella pneumoniae
IMP-38
ST307
whole-genome sequencing
plasmid
high-risk clone
Microbiology
QR1-502
Acceso en línea:https://doaj.org/article/1feb4fcd86fe4c75bcbd037e8f6f2f6e
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spelling oai:doaj.org-article:1feb4fcd86fe4c75bcbd037e8f6f2f6e2021-11-15T15:30:50ZIMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China10.1128/mSphere.00407-202379-5042https://doaj.org/article/1feb4fcd86fe4c75bcbd037e8f6f2f6e2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00407-20https://doaj.org/toc/2379-5042ABSTRACT An emerging multidrug-resistant Klebsiella pneumoniae high-risk clone of sequence type 307 (ST307) has been increasingly reported worldwide. Here, we described the genomic characteristics of an IMP-38-producing ST307 K. pneumoniae strain and investigated the prevalence of blaIMP-38 among carbapenem-resistant Klebsiella pneumoniae isolates from a tertiary care hospital in central China. A total of 14 IMP-38-producing ST307 K. pneumoniae strains were identified from 2013 to 2016, with 13 strains isolated from patients with neonatal sepsis in the neonatal ward. PacBio and Illumina whole-genome sequencing analysis performed on a representative IMP-38-producing K. pneumoniae strain, WCGKP294, showed that it contained a circular chromosome and two plasmids. Carbapenemase gene blaIMP-38 is colocated with blaCTX-M-3 in transposon Tn6382 on an IncHI5 plasmid (pWCGKP294-2). WCGKP294 harbors another IncFIB plasmid, pWCGKP294-1, carrying three copies of tandem-repeated IS26-blaSHV-2A-deoR-ygbJ-ygbK-fucA-IS26 composite transposon elements. Phylogenetic analysis placed WCGKP294 in the global ST307 cluster, distant from the U.S. (Texas) and South Africa clusters. Nevertheless, WCGKP294 does not contain the chromosomal fluoroquinolone resistance-associated mutations and IncFIIK/IncFIBK plasmid-associated blaCTX-M-15 gene that are frequently found in other global ST307 strains. IMPORTANCE We described the genome and resistome characterization of a carbapenem-resistant Klebsiella pneumoniae ST307 strain carrying blaIMP-38 in China. This report highlights that the high-risk ST307 clone continues to acquire different antimicrobial resistance genes, posing significant challenges to clinical practice, and should be closely monitored.Siyi WangJuan ZhaoNing LiuFang YangYiming ZhongXiumei GuZijuan JianQun YanQingxia LiuHongling LiYanming LiJing LiuHui LiLiang ChenWenen LiuAmerican Society for Microbiologyarticlecarbapenem-resistant Klebsiella pneumoniaeIMP-38ST307whole-genome sequencingplasmidhigh-risk cloneMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution DOAJ
collection DOAJ
language EN
topic carbapenem-resistant Klebsiella pneumoniae
IMP-38
ST307
whole-genome sequencing
plasmid
high-risk clone
Microbiology
QR1-502
spellingShingle carbapenem-resistant Klebsiella pneumoniae
IMP-38
ST307
whole-genome sequencing
plasmid
high-risk clone
Microbiology
QR1-502
Siyi Wang
Juan Zhao
Ning Liu
Fang Yang
Yiming Zhong
Xiumei Gu
Zijuan Jian
Qun Yan
Qingxia Liu
Hongling Li
Yanming Li
Jing Liu
Hui Li
Liang Chen
Wenen Liu
IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China
description ABSTRACT An emerging multidrug-resistant Klebsiella pneumoniae high-risk clone of sequence type 307 (ST307) has been increasingly reported worldwide. Here, we described the genomic characteristics of an IMP-38-producing ST307 K. pneumoniae strain and investigated the prevalence of blaIMP-38 among carbapenem-resistant Klebsiella pneumoniae isolates from a tertiary care hospital in central China. A total of 14 IMP-38-producing ST307 K. pneumoniae strains were identified from 2013 to 2016, with 13 strains isolated from patients with neonatal sepsis in the neonatal ward. PacBio and Illumina whole-genome sequencing analysis performed on a representative IMP-38-producing K. pneumoniae strain, WCGKP294, showed that it contained a circular chromosome and two plasmids. Carbapenemase gene blaIMP-38 is colocated with blaCTX-M-3 in transposon Tn6382 on an IncHI5 plasmid (pWCGKP294-2). WCGKP294 harbors another IncFIB plasmid, pWCGKP294-1, carrying three copies of tandem-repeated IS26-blaSHV-2A-deoR-ygbJ-ygbK-fucA-IS26 composite transposon elements. Phylogenetic analysis placed WCGKP294 in the global ST307 cluster, distant from the U.S. (Texas) and South Africa clusters. Nevertheless, WCGKP294 does not contain the chromosomal fluoroquinolone resistance-associated mutations and IncFIIK/IncFIBK plasmid-associated blaCTX-M-15 gene that are frequently found in other global ST307 strains. IMPORTANCE We described the genome and resistome characterization of a carbapenem-resistant Klebsiella pneumoniae ST307 strain carrying blaIMP-38 in China. This report highlights that the high-risk ST307 clone continues to acquire different antimicrobial resistance genes, posing significant challenges to clinical practice, and should be closely monitored.
format article
author Siyi Wang
Juan Zhao
Ning Liu
Fang Yang
Yiming Zhong
Xiumei Gu
Zijuan Jian
Qun Yan
Qingxia Liu
Hongling Li
Yanming Li
Jing Liu
Hui Li
Liang Chen
Wenen Liu
author_facet Siyi Wang
Juan Zhao
Ning Liu
Fang Yang
Yiming Zhong
Xiumei Gu
Zijuan Jian
Qun Yan
Qingxia Liu
Hongling Li
Yanming Li
Jing Liu
Hui Li
Liang Chen
Wenen Liu
author_sort Siyi Wang
title IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China
title_short IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China
title_full IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China
title_fullStr IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China
title_full_unstemmed IMP-38-Producing High-Risk Sequence Type 307 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Strains from a Neonatal Unit in China
title_sort imp-38-producing high-risk sequence type 307 <named-content content-type="genus-species">klebsiella pneumoniae</named-content> strains from a neonatal unit in china
publisher American Society for Microbiology
publishDate 2020
url https://doaj.org/article/1feb4fcd86fe4c75bcbd037e8f6f2f6e
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