Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.

Intense exercise is a physiological stress capable of inducing the interaction of neutrophils with muscle endothelial cells and their transmigration into tissue. Mechanisms driving this physiological inflammatory response are not known. Here, we investigate whether production of reactive oxygen spec...

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Autores principales:	Albená Nunes-Silva, Priscila T T Bernardes, Bárbara M Rezende, Fernando Lopes, Elisa C Gomes, Pedro E Marques, Paulo M A Lima, Cândido C Coimbra, Gustavo B Menezes, Mauro M Teixeira, Vanessa Pinho
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1fee052de2804a28b70ae0f4141ec8d8
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spelling	oai:doaj.org-article:1fee052de2804a28b70ae0f4141ec8d82021-11-18T08:20:45ZTreadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.1932-620310.1371/journal.pone.0096464https://doaj.org/article/1fee052de2804a28b70ae0f4141ec8d82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24798414/?tool=EBIhttps://doaj.org/toc/1932-6203Intense exercise is a physiological stress capable of inducing the interaction of neutrophils with muscle endothelial cells and their transmigration into tissue. Mechanisms driving this physiological inflammatory response are not known. Here, we investigate whether production of reactive oxygen species is relevant for neutrophil interaction with endothelial cells and recruitment into the quadriceps muscle in mice subjected to the treadmill fatiguing exercise protocol. Mice exercised until fatigue by running for 56.3±6.8 min on an electric treadmill. Skeletal muscle was evaluated by intravital microscopy at different time points after exercise, and then removed to assess local oxidative stress and histopathological analysis. We observed an increase in plasma lactate and creatine kinase (CK) concentrations after exercise. The numbers of monocytes, neutrophils, and lymphocytes in blood increased 12 and 24 hours after the exercise. Numbers of rolling and adherent leukocytes increased 3, 6, 12, and 24 hours post-exercise, as assessed by intravital microscopy. Using LysM-eGFP mice and confocal intravital microscopy technology, we show that the number of transmigrating neutrophils increased 12 hours post-exercise. Mutant gp91phox-/- (non-functional NADPH oxidase) mice and mice treated with apocynin showed diminished neutrophil recruitment. SOD treatment promoted further adhesion and transmigration of leukocytes 12 hours after the exercise. These findings confirm our hypothesis that treadmill exercise increases the recruitment of leukocytes to the postcapillary venules, and NADPH oxidase-induced ROS plays an important role in this process.Albená Nunes-SilvaPriscila T T BernardesBárbara M RezendeFernando LopesElisa C GomesPedro E MarquesPaulo M A LimaCândido C CoimbraGustavo B MenezesMauro M TeixeiraVanessa PinhoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96464 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Albená Nunes-Silva Priscila T T Bernardes Bárbara M Rezende Fernando Lopes Elisa C Gomes Pedro E Marques Paulo M A Lima Cândido C Coimbra Gustavo B Menezes Mauro M Teixeira Vanessa Pinho Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.
description	Intense exercise is a physiological stress capable of inducing the interaction of neutrophils with muscle endothelial cells and their transmigration into tissue. Mechanisms driving this physiological inflammatory response are not known. Here, we investigate whether production of reactive oxygen species is relevant for neutrophil interaction with endothelial cells and recruitment into the quadriceps muscle in mice subjected to the treadmill fatiguing exercise protocol. Mice exercised until fatigue by running for 56.3±6.8 min on an electric treadmill. Skeletal muscle was evaluated by intravital microscopy at different time points after exercise, and then removed to assess local oxidative stress and histopathological analysis. We observed an increase in plasma lactate and creatine kinase (CK) concentrations after exercise. The numbers of monocytes, neutrophils, and lymphocytes in blood increased 12 and 24 hours after the exercise. Numbers of rolling and adherent leukocytes increased 3, 6, 12, and 24 hours post-exercise, as assessed by intravital microscopy. Using LysM-eGFP mice and confocal intravital microscopy technology, we show that the number of transmigrating neutrophils increased 12 hours post-exercise. Mutant gp91phox-/- (non-functional NADPH oxidase) mice and mice treated with apocynin showed diminished neutrophil recruitment. SOD treatment promoted further adhesion and transmigration of leukocytes 12 hours after the exercise. These findings confirm our hypothesis that treadmill exercise increases the recruitment of leukocytes to the postcapillary venules, and NADPH oxidase-induced ROS plays an important role in this process.
format	article
author	Albená Nunes-Silva Priscila T T Bernardes Bárbara M Rezende Fernando Lopes Elisa C Gomes Pedro E Marques Paulo M A Lima Cândido C Coimbra Gustavo B Menezes Mauro M Teixeira Vanessa Pinho
author_facet	Albená Nunes-Silva Priscila T T Bernardes Bárbara M Rezende Fernando Lopes Elisa C Gomes Pedro E Marques Paulo M A Lima Cândido C Coimbra Gustavo B Menezes Mauro M Teixeira Vanessa Pinho
author_sort	Albená Nunes-Silva
title	Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.
title_short	Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.
title_full	Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.
title_fullStr	Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.
title_full_unstemmed	Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.
title_sort	treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. an intravital microscopy study.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1fee052de2804a28b70ae0f4141ec8d8
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Treadmill exercise induces neutrophil recruitment into muscle tissue in a reactive oxygen species-dependent manner. An intravital microscopy study.

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