The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis

Introduction: Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective: to assess the effect of topical Aloe vera e...

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Autores principales:	Kusumawati Soetrisno, Prasetyowati Subchan, Atina Hussana
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2021
Materias:	ultraviolet b, aloe vera, sunburn cell, caspase-3 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1ff1c6e382f04af6b4a6a35bec04fc7b
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spelling	oai:doaj.org-article:1ff1c6e382f04af6b4a6a35bec04fc7b2021-11-25T13:16:45ZThe Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis2085-15452339-093X10.30659/sainsmed.v11i2.9094https://doaj.org/article/1ff1c6e382f04af6b4a6a35bec04fc7b2021-01-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/9094https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction: Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective: to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure. Methods: In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test. Results: The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05. Conclusion: Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.Kusumawati SoetrisnoPrasetyowati SubchanAtina HussanaUniversitas Sultan Agung Semarangarticleultraviolet b, aloe vera, sunburn cell, caspase-3Medicine (General)R5-920ENSains Medika, Vol 11, Iss 2, Pp 89-96 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ultraviolet b, aloe vera, sunburn cell, caspase-3 Medicine (General) R5-920
spellingShingle	ultraviolet b, aloe vera, sunburn cell, caspase-3 Medicine (General) R5-920 Kusumawati Soetrisno Prasetyowati Subchan Atina Hussana The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
description	Introduction: Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective: to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure. Methods: In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test. Results: The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05. Conclusion: Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.
format	article
author	Kusumawati Soetrisno Prasetyowati Subchan Atina Hussana
author_facet	Kusumawati Soetrisno Prasetyowati Subchan Atina Hussana
author_sort	Kusumawati Soetrisno
title	The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
title_short	The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
title_full	The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
title_fullStr	The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
title_full_unstemmed	The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
title_sort	administration of topical aloe vera extract reduce the number of sunburn cells and expression of caspase-3 on post uvb-light-exposure epidermis
publisher	Universitas Sultan Agung Semarang
publishDate	2021
url	https://doaj.org/article/1ff1c6e382f04af6b4a6a35bec04fc7b
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The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis

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