Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.

In this paper, we report on the large-scale formation of supramolecular rhombus microparticles (SRMs) driven by electrostatic assembly, carried out by direct mixing of an aqueous HAuCl(4) solution and an ethanol solution of 4,4'-bipyridine at room temperature. We further demonstrate their use a...

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Autores principales:	Hailong Li, Junfeng Zhai, Xuping Sun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ff2321038a44dcda6d65f6e385f76c8
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spelling	oai:doaj.org-article:1ff2321038a44dcda6d65f6e385f76c82021-11-18T06:55:35ZElectrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.1932-620310.1371/journal.pone.0018958https://doaj.org/article/1ff2321038a44dcda6d65f6e385f76c82011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21526152/?tool=EBIhttps://doaj.org/toc/1932-6203In this paper, we report on the large-scale formation of supramolecular rhombus microparticles (SRMs) driven by electrostatic assembly, carried out by direct mixing of an aqueous HAuCl(4) solution and an ethanol solution of 4,4'-bipyridine at room temperature. We further demonstrate their use as an effective fluorescent sensing platform for nucleic acid detection with a high selectivity down to single-base mismatch. The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by SRM, which is accompanied by substantial fluorescence quenching. In the following assay, specific hybridization with its target to form double-stranded DNA (dsDNA) results in desorption of ssDNA from SRM surface and subsequent fluorescence recovery.Hailong LiJunfeng ZhaiXuping SunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18958 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hailong Li Junfeng Zhai Xuping Sun Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
description	In this paper, we report on the large-scale formation of supramolecular rhombus microparticles (SRMs) driven by electrostatic assembly, carried out by direct mixing of an aqueous HAuCl(4) solution and an ethanol solution of 4,4'-bipyridine at room temperature. We further demonstrate their use as an effective fluorescent sensing platform for nucleic acid detection with a high selectivity down to single-base mismatch. The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by SRM, which is accompanied by substantial fluorescence quenching. In the following assay, specific hybridization with its target to form double-stranded DNA (dsDNA) results in desorption of ssDNA from SRM surface and subsequent fluorescence recovery.
format	article
author	Hailong Li Junfeng Zhai Xuping Sun
author_facet	Hailong Li Junfeng Zhai Xuping Sun
author_sort	Hailong Li
title	Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
title_short	Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
title_full	Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
title_fullStr	Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
title_full_unstemmed	Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
title_sort	electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1ff2321038a44dcda6d65f6e385f76c8
work_keys_str_mv	AT hailongli electrostaticassemblydrivenformationofsupramolecularrhombusmicroparticlesandtheirapplicationforfluorescentnucleicaciddetection AT junfengzhai electrostaticassemblydrivenformationofsupramolecularrhombusmicroparticlesandtheirapplicationforfluorescentnucleicaciddetection AT xupingsun electrostaticassemblydrivenformationofsupramolecularrhombusmicroparticlesandtheirapplicationforfluorescentnucleicaciddetection
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Electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detection.

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