Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.

In the present study we evaluated the protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzi ´s amastigote surface protein 2 (ASP2). Those viruses were used in sequenti...

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Autores principales:	Rafael Polidoro Alves Barbosa, Bruno Galvão Filho, Luara Isabela Dos Santos, Policarpo Ademar Sales Junior, Pedro Elias Marques, Rafaela Vaz Sousa Pereira, Denise Carmona Cara, Oscar Bruña-Romero, Maurício Martins Rodrigues, Ricardo Tostes Gazzinelli, Alexandre Vieira Machado
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ff29b3b4b6041dbb2b381180542c873
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spelling	oai:doaj.org-article:1ff29b3b4b6041dbb2b381180542c8732021-11-18T07:48:02ZVaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.1932-620310.1371/journal.pone.0061795https://doaj.org/article/1ff29b3b4b6041dbb2b381180542c8732013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637908/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In the present study we evaluated the protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzi ´s amastigote surface protein 2 (ASP2). Those viruses were used in sequential immunization with recombinant adenovirus (heterologous prime-boost immunization protocol) encoding the complete sequence of ASP2 (Ad-ASP2) in two mouse strains (C57BL/6 and C3H/He). The CD8 effector response elicited by this protocol was comparable to that observed in mice immunized twice with Ad-ASP2 and more robust than that observed in mice that were immunized once with Ad-ASP2. Whereas a single immunization with Ad-ASP2 sufficed to completely protect C57BL/6 mice, a higher survival rate was observed in C3H/He mice that were primed with recombinant influenza virus and boosted with Ad-ASP2 after being challenged with T. cruzi. Analyzing the phenotype of CD8+ T cells obtained from spleen of vaccinated C3H/He mice we observed that heterologous prime-boost immunization protocol elicited more CD8+ T cells specific for the immunodominant epitope as well as a higher number of CD8+ T cells producing TNF-α and IFN-γ and a higher mobilization of surface marker CD107a. Taken together, our results suggest that immunodominant subpopulations of CD8+ T elicited after immunization could be directly related to degree of protection achieved by different immunization protocols using different viral vectors. Overall, these results demonstrated the usefulness of recombinant influenza viruses in immunization protocols against Chagas Disease.Rafael Polidoro Alves BarbosaBruno Galvão FilhoLuara Isabela Dos SantosPolicarpo Ademar Sales JuniorPedro Elias MarquesRafaela Vaz Sousa PereiraDenise Carmona CaraOscar Bruña-RomeroMaurício Martins RodriguesRicardo Tostes GazzinelliAlexandre Vieira MachadoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61795 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rafael Polidoro Alves Barbosa Bruno Galvão Filho Luara Isabela Dos Santos Policarpo Ademar Sales Junior Pedro Elias Marques Rafaela Vaz Sousa Pereira Denise Carmona Cara Oscar Bruña-Romero Maurício Martins Rodrigues Ricardo Tostes Gazzinelli Alexandre Vieira Machado Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.
description	In the present study we evaluated the protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzi ´s amastigote surface protein 2 (ASP2). Those viruses were used in sequential immunization with recombinant adenovirus (heterologous prime-boost immunization protocol) encoding the complete sequence of ASP2 (Ad-ASP2) in two mouse strains (C57BL/6 and C3H/He). The CD8 effector response elicited by this protocol was comparable to that observed in mice immunized twice with Ad-ASP2 and more robust than that observed in mice that were immunized once with Ad-ASP2. Whereas a single immunization with Ad-ASP2 sufficed to completely protect C57BL/6 mice, a higher survival rate was observed in C3H/He mice that were primed with recombinant influenza virus and boosted with Ad-ASP2 after being challenged with T. cruzi. Analyzing the phenotype of CD8+ T cells obtained from spleen of vaccinated C3H/He mice we observed that heterologous prime-boost immunization protocol elicited more CD8+ T cells specific for the immunodominant epitope as well as a higher number of CD8+ T cells producing TNF-α and IFN-γ and a higher mobilization of surface marker CD107a. Taken together, our results suggest that immunodominant subpopulations of CD8+ T elicited after immunization could be directly related to degree of protection achieved by different immunization protocols using different viral vectors. Overall, these results demonstrated the usefulness of recombinant influenza viruses in immunization protocols against Chagas Disease.
format	article
author	Rafael Polidoro Alves Barbosa Bruno Galvão Filho Luara Isabela Dos Santos Policarpo Ademar Sales Junior Pedro Elias Marques Rafaela Vaz Sousa Pereira Denise Carmona Cara Oscar Bruña-Romero Maurício Martins Rodrigues Ricardo Tostes Gazzinelli Alexandre Vieira Machado
author_facet	Rafael Polidoro Alves Barbosa Bruno Galvão Filho Luara Isabela Dos Santos Policarpo Ademar Sales Junior Pedro Elias Marques Rafaela Vaz Sousa Pereira Denise Carmona Cara Oscar Bruña-Romero Maurício Martins Rodrigues Ricardo Tostes Gazzinelli Alexandre Vieira Machado
author_sort	Rafael Polidoro Alves Barbosa
title	Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.
title_short	Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.
title_full	Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.
title_fullStr	Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.
title_full_unstemmed	Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.
title_sort	vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against trypanosoma cruzi infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1ff29b3b4b6041dbb2b381180542c873
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Vaccination using recombinants influenza and adenoviruses encoding amastigote surface protein-2 are highly effective on protection against Trypanosoma cruzi infection.

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