PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

Ming-Hua Ho,1 Hao-Chieh Chang,2,3 Yu-Chia Chang,3 Jeiannete Claudia,1 Tzu-Chiao Lin,2 Po-Chun Chang2,3 1Department of Chemical Engineering, College of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan; 2Graduate Institute of Clinical Dentistry, School of Dentistry, N...

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Autores principales:	Ho MH, Chang H, Chang Y, Claudia J, Lin TC, Chang PC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Tissue engineering platelet-derived growth factor metronidazole alveolar process animal models Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1ff5eddfe65140f797e1d9dd5e890c14
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spelling	oai:doaj.org-article:1ff5eddfe65140f797e1d9dd5e890c142021-12-02T00:09:56ZPDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration1178-2013https://doaj.org/article/1ff5eddfe65140f797e1d9dd5e890c142017-08-01T00:00:00Zhttps://www.dovepress.com/pdgf-metronidazole-encapsulated-nanofibrous-functional-layers-on-colla-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ming-Hua Ho,1 Hao-Chieh Chang,2,3 Yu-Chia Chang,3 Jeiannete Claudia,1 Tzu-Chiao Lin,2 Po-Chun Chang2,3 1Department of Chemical Engineering, College of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan; 2Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; 3Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan Abstract: This study aimed to develop a functionally graded membrane (FGM) to prevent infection and promote tissue regeneration. Poly(L-lactide-co-D,L-lactide) encapsulating platelet-derived growth factor (PDLLA-PDGF) or metronidazole (PDLLA-MTZ) was electrospun to form a nanofibrous layer on the inner or outer surface of a clinically available collagen membrane, respectively. The membrane was characterized for the morphology, molecule release profile, in vitro and in vivo biocompatibility, and preclinical efficiency for alveolar ridge regeneration. The PDLLA-MTZ and PDLLA-PDGF nanofibers were 800–900 nm in diameter, and the thicknesses of the functional layers were 20–30 µm, with sustained molecule release over 28 days. All of the membranes tested were compatible with cell survival in vitro and showed good tissue integration with minimal fibrous capsule formation or inflammation. Cell proliferation was especially prominent on the PDLLA-PDGF layer in vivo. On the alveolar ridge, all FGMs reduced wound dehiscence compared with the control collagen membrane, and the FGM with PDLLA-PDGF promoted osteogenesis significantly. In conclusion, the FGMs with PDLLA-PDGF and PDLLA-MTZ showed high biocompatibility and facilitated wound healing compared with conventional membrane, and the FGM with PDLLA-PDGF enhanced alveolar ridge regeneration in vivo. The design represents a beneficial modification, which may be easily adapted for future clinical use. Keywords: tissue engineering, platelet-derived growth factor, metronidazole, alveolar process, animal modelsHo MHChang HChang YClaudia JLin TCChang PCDove Medical PressarticleTissue engineeringplatelet-derived growth factormetronidazolealveolar processanimal modelsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5525-5535 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tissue engineering platelet-derived growth factor metronidazole alveolar process animal models Medicine (General) R5-920
spellingShingle	Tissue engineering platelet-derived growth factor metronidazole alveolar process animal models Medicine (General) R5-920 Ho MH Chang H Chang Y Claudia J Lin TC Chang PC PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
description	Ming-Hua Ho,1 Hao-Chieh Chang,2,3 Yu-Chia Chang,3 Jeiannete Claudia,1 Tzu-Chiao Lin,2 Po-Chun Chang2,3 1Department of Chemical Engineering, College of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan; 2Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; 3Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan Abstract: This study aimed to develop a functionally graded membrane (FGM) to prevent infection and promote tissue regeneration. Poly(L-lactide-co-D,L-lactide) encapsulating platelet-derived growth factor (PDLLA-PDGF) or metronidazole (PDLLA-MTZ) was electrospun to form a nanofibrous layer on the inner or outer surface of a clinically available collagen membrane, respectively. The membrane was characterized for the morphology, molecule release profile, in vitro and in vivo biocompatibility, and preclinical efficiency for alveolar ridge regeneration. The PDLLA-MTZ and PDLLA-PDGF nanofibers were 800–900 nm in diameter, and the thicknesses of the functional layers were 20–30 µm, with sustained molecule release over 28 days. All of the membranes tested were compatible with cell survival in vitro and showed good tissue integration with minimal fibrous capsule formation or inflammation. Cell proliferation was especially prominent on the PDLLA-PDGF layer in vivo. On the alveolar ridge, all FGMs reduced wound dehiscence compared with the control collagen membrane, and the FGM with PDLLA-PDGF promoted osteogenesis significantly. In conclusion, the FGMs with PDLLA-PDGF and PDLLA-MTZ showed high biocompatibility and facilitated wound healing compared with conventional membrane, and the FGM with PDLLA-PDGF enhanced alveolar ridge regeneration in vivo. The design represents a beneficial modification, which may be easily adapted for future clinical use. Keywords: tissue engineering, platelet-derived growth factor, metronidazole, alveolar process, animal models
format	article
author	Ho MH Chang H Chang Y Claudia J Lin TC Chang PC
author_facet	Ho MH Chang H Chang Y Claudia J Lin TC Chang PC
author_sort	Ho MH
title	PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
title_short	PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
title_full	PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
title_fullStr	PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
title_full_unstemmed	PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
title_sort	pdgf-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/1ff5eddfe65140f797e1d9dd5e890c14
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PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

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