Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.

<h4>Background</h4>Bacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assessed the impact of diabetes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lance B Price, Cindy M Liu, Johan H Melendez, Yelena M Frankel, David Engelthaler, Maliha Aziz, Jolene Bowers, Rogan Rattray, Jacques Ravel, Chris Kingsley, Paul S Keim, Gerald S Lazarus, Jonathan M Zenilman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ffe18b194f74ecf9103a1faefa29437
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1ffe18b194f74ecf9103a1faefa29437
record_format	dspace
spelling	oai:doaj.org-article:1ffe18b194f74ecf9103a1faefa294372021-11-25T06:21:17ZCommunity analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.1932-620310.1371/journal.pone.0006462https://doaj.org/article/1ffe18b194f74ecf9103a1faefa294372009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19649281/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assessed the impact of diabetes and antibiotics on chronic wound microbiota.<h4>Methodology/principal findings</h4>We prospectively enrolled 24 patients at a referral wound center in Baltimore, MD; sampled patients' wounds by curette; cultured samples under aerobic and anaerobic conditions; and pyrosequenced the 16S rRNA V3 hypervariable region. The 16S rRNA gene-based analyses revealed an average of 10 different bacterial families in wounds--approximately 4 times more than estimated by culture-based analyses. Fastidious anaerobic bacteria belonging to the Clostridiales family XI were among the most prevalent bacteria identified exclusively by 16S rRNA gene-based analyses. Community-scale analyses showed that wound microbiota from antibiotic treated patients were significantly different from untreated patients (p = 0.007) and were characterized by increased Pseudomonadaceae abundance. These analyses also revealed that antibiotic use was associated with decreased Streptococcaceae among diabetics and that Streptococcaceae was more abundant among diabetics as compared to non-diabetics.<h4>Conclusions/significance</h4>The 16S rRNA gene-based analyses revealed complex bacterial communities including anaerobic bacteria that may play causative roles in the non-healing state of some chronic wounds. Our data suggest that antimicrobial therapy alters community structure--reducing some bacteria while selecting for others.Lance B PriceCindy M LiuJohan H MelendezYelena M FrankelDavid EngelthalerMaliha AzizJolene BowersRogan RattrayJacques RavelChris KingsleyPaul S KeimGerald S LazarusJonathan M ZenilmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6462 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lance B Price Cindy M Liu Johan H Melendez Yelena M Frankel David Engelthaler Maliha Aziz Jolene Bowers Rogan Rattray Jacques Ravel Chris Kingsley Paul S Keim Gerald S Lazarus Jonathan M Zenilman Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
description	<h4>Background</h4>Bacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assessed the impact of diabetes and antibiotics on chronic wound microbiota.<h4>Methodology/principal findings</h4>We prospectively enrolled 24 patients at a referral wound center in Baltimore, MD; sampled patients' wounds by curette; cultured samples under aerobic and anaerobic conditions; and pyrosequenced the 16S rRNA V3 hypervariable region. The 16S rRNA gene-based analyses revealed an average of 10 different bacterial families in wounds--approximately 4 times more than estimated by culture-based analyses. Fastidious anaerobic bacteria belonging to the Clostridiales family XI were among the most prevalent bacteria identified exclusively by 16S rRNA gene-based analyses. Community-scale analyses showed that wound microbiota from antibiotic treated patients were significantly different from untreated patients (p = 0.007) and were characterized by increased Pseudomonadaceae abundance. These analyses also revealed that antibiotic use was associated with decreased Streptococcaceae among diabetics and that Streptococcaceae was more abundant among diabetics as compared to non-diabetics.<h4>Conclusions/significance</h4>The 16S rRNA gene-based analyses revealed complex bacterial communities including anaerobic bacteria that may play causative roles in the non-healing state of some chronic wounds. Our data suggest that antimicrobial therapy alters community structure--reducing some bacteria while selecting for others.
format	article
author	Lance B Price Cindy M Liu Johan H Melendez Yelena M Frankel David Engelthaler Maliha Aziz Jolene Bowers Rogan Rattray Jacques Ravel Chris Kingsley Paul S Keim Gerald S Lazarus Jonathan M Zenilman
author_facet	Lance B Price Cindy M Liu Johan H Melendez Yelena M Frankel David Engelthaler Maliha Aziz Jolene Bowers Rogan Rattray Jacques Ravel Chris Kingsley Paul S Keim Gerald S Lazarus Jonathan M Zenilman
author_sort	Lance B Price
title	Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
title_short	Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
title_full	Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
title_fullStr	Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
title_full_unstemmed	Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
title_sort	community analysis of chronic wound bacteria using 16s rrna gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/1ffe18b194f74ecf9103a1faefa29437
work_keys_str_mv	AT lancebprice communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT cindymliu communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT johanhmelendez communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT yelenamfrankel communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT davidengelthaler communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT malihaaziz communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT jolenebowers communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT roganrattray communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT jacquesravel communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT chriskingsley communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT paulskeim communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT geraldslazarus communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota AT jonathanmzenilman communityanalysisofchronicwoundbacteriausing16srrnagenebasedpyrosequencingimpactofdiabetesandantibioticsonchronicwoundmicrobiota
_version_	1718413791144706048

Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.

Ejemplares similares