Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars

Yang Liu,1,* Wei Huang,2,* Chuanfeng Xiong,2 Yuxian Huang,2 Benny J Chen,2,3 Luigi Racioppi,2,4 Nelson Chao,2,3 Tuan Vo-Dinh1,3,5–61Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; 2Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA;...

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Autores principales:	Liu Y, Huang W, Xiong C, Huang Y, Chen BJ, Racioppi L, Chao N, Vo-Dinh T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	GNS ICP-MS Two-photon microscopy Immune cells Biodistribution Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2000e5258cee437da8599f500d3c7d64
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spelling	oai:doaj.org-article:2000e5258cee437da8599f500d3c7d642021-12-02T05:27:12ZBiodistribution and sensitive tracking of immune cells with plasmonic gold nanostars1178-2013https://doaj.org/article/2000e5258cee437da8599f500d3c7d642019-05-01T00:00:00Zhttps://www.dovepress.com/biodistribution-and-sensitive-tracking-of-immune-cells-with-plasmonic--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yang Liu,1,* Wei Huang,2,* Chuanfeng Xiong,2 Yuxian Huang,2 Benny J Chen,2,3 Luigi Racioppi,2,4 Nelson Chao,2,3 Tuan Vo-Dinh1,3,5–61Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; 2Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA; 3Duke Cancer Institute, Duke University Medical Center, Durham, NC 27710, USA; 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples 80131, Italy; 5Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA; 6Department of Chemistry, Duke University, Durham, NC 27708, USA*These authors contributed equally to this work Aim: To quantitatively and sensitively investigate the biodistribution of immune cells after systemic administration.Methods: Immune cells were loaded with plasmonic gold nanostars (GNS) tracking probes. Inductively coupled plasma mass spectrometry (ICP-MS) was used for quantitative gold mass measurement and two-photon photoluminescence (TPL) was used for high-resolution sensitive optical imaging.Results: GNS nanoparticles were loaded successfully into immune cells without negative effect on cellular vitality. Liver and spleen were identified to be the major organs for macrophage cells uptake after systematic administration. A small amount of macrophage cells were detected in the tumor site in our murine lymphoma animal model.Conclusion: GNS has great potential as a biocompatible marker for quantitative tracking and high-resolution imaging of immune cells at the cellular level.Keywords: GNS, ICP-MS, two-photon microscopy, immune cells, biodistribution  Liu YHuang WXiong CHuang YChen BJRacioppi LChao NVo-Dinh TDove Medical PressarticleGNSICP-MSTwo-photon microscopyImmune cellsBiodistributionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3403-3411 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	GNS ICP-MS Two-photon microscopy Immune cells Biodistribution Medicine (General) R5-920
spellingShingle	GNS ICP-MS Two-photon microscopy Immune cells Biodistribution Medicine (General) R5-920 Liu Y Huang W Xiong C Huang Y Chen BJ Racioppi L Chao N Vo-Dinh T Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
description	Yang Liu,1,* Wei Huang,2,* Chuanfeng Xiong,2 Yuxian Huang,2 Benny J Chen,2,3 Luigi Racioppi,2,4 Nelson Chao,2,3 Tuan Vo-Dinh1,3,5–61Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; 2Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA; 3Duke Cancer Institute, Duke University Medical Center, Durham, NC 27710, USA; 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples 80131, Italy; 5Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA; 6Department of Chemistry, Duke University, Durham, NC 27708, USA*These authors contributed equally to this work Aim: To quantitatively and sensitively investigate the biodistribution of immune cells after systemic administration.Methods: Immune cells were loaded with plasmonic gold nanostars (GNS) tracking probes. Inductively coupled plasma mass spectrometry (ICP-MS) was used for quantitative gold mass measurement and two-photon photoluminescence (TPL) was used for high-resolution sensitive optical imaging.Results: GNS nanoparticles were loaded successfully into immune cells without negative effect on cellular vitality. Liver and spleen were identified to be the major organs for macrophage cells uptake after systematic administration. A small amount of macrophage cells were detected in the tumor site in our murine lymphoma animal model.Conclusion: GNS has great potential as a biocompatible marker for quantitative tracking and high-resolution imaging of immune cells at the cellular level.Keywords: GNS, ICP-MS, two-photon microscopy, immune cells, biodistribution
format	article
author	Liu Y Huang W Xiong C Huang Y Chen BJ Racioppi L Chao N Vo-Dinh T
author_facet	Liu Y Huang W Xiong C Huang Y Chen BJ Racioppi L Chao N Vo-Dinh T
author_sort	Liu Y
title	Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
title_short	Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
title_full	Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
title_fullStr	Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
title_full_unstemmed	Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
title_sort	biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/2000e5258cee437da8599f500d3c7d64
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Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars

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